Experiment: LB; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cheV-2 and SO3124 are separated by 137 nucleotides SO3124 and SO3125 are separated by 66 nucleotides SO3125 and ogt are separated by 37 nucleotides ogt and SO3127 are separated by 105 nucleotides
SO3123: cheV-2 - chemotaxis protein CheV (NCBI ptt file), at 3,250,827 to 3,251,726
cheV-2
SO3124: SO3124 - tyrosine-specific protein phosphatase, putative (NCBI ptt file), at 3,251,864 to 3,252,334
SO3124
SO3125: SO3125 - ATP-dependent RNA helicase, DEAD box family (NCBI ptt file), at 3,252,401 to 3,253,684
SO3125
SO3126: ogt - methylated-DNA--protein-cysteine methyltransferase (NCBI ptt file), at 3,253,722 to 3,254,225
ogt
SO3127: SO3127 - Ada regulatory protein, putative (NCBI ptt file), at 3,254,331 to 3,255,959
SO3127
Position (kb)
3252
3253
3254 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3251.454 kb on + strand, within cheV-2 at 3251.498 kb on + strand, within cheV-2 at 3251.698 kb on - strand at 3251.764 kb on + strand at 3251.764 kb on + strand at 3251.764 kb on + strand at 3251.772 kb on - strand at 3251.772 kb on - strand at 3251.772 kb on - strand at 3251.789 kb on - strand at 3251.800 kb on + strand at 3251.808 kb on - strand at 3251.856 kb on - strand at 3251.856 kb on - strand at 3251.885 kb on - strand at 3251.968 kb on - strand, within SO3124 at 3252.060 kb on + strand, within SO3124 at 3252.189 kb on - strand, within SO3124 at 3252.196 kb on + strand, within SO3124 at 3252.229 kb on - strand, within SO3124 at 3252.239 kb on - strand, within SO3124 at 3252.287 kb on + strand, within SO3124 at 3252.445 kb on - strand at 3252.469 kb on + strand at 3252.513 kb on - strand at 3252.671 kb on + strand, within SO3125 at 3252.815 kb on + strand, within SO3125 at 3252.833 kb on + strand, within SO3125 at 3252.836 kb on + strand, within SO3125 at 3253.121 kb on + strand, within SO3125 at 3253.139 kb on - strand, within SO3125 at 3253.338 kb on + strand, within SO3125 at 3253.440 kb on - strand, within SO3125 at 3253.456 kb on - strand, within SO3125 at 3253.582 kb on - strand at 3253.582 kb on - strand at 3253.589 kb on + strand at 3253.589 kb on + strand at 3253.886 kb on + strand, within ogt at 3253.897 kb on + strand, within ogt at 3254.038 kb on + strand, within ogt at 3254.230 kb on - strand at 3254.456 kb on + strand at 3254.458 kb on + strand at 3254.519 kb on + strand, within SO3127 at 3254.566 kb on + strand, within SO3127 at 3254.566 kb on + strand, within SO3127 at 3254.566 kb on + strand, within SO3127 at 3254.613 kb on + strand, within SO3127 at 3254.670 kb on + strand, within SO3127
Per-strain Table
Position Strand Gene LocusTag Fraction LB; anaerobic remove 3,251,454 + cheV-2 SO3123 0.70 +0.6 3,251,498 + cheV-2 SO3123 0.75 +0.4 3,251,698 - +1.1 3,251,764 + -0.2 3,251,764 + +0.4 3,251,764 + +0.4 3,251,772 - -0.4 3,251,772 - -1.1 3,251,772 - +0.0 3,251,789 - +1.6 3,251,800 + +0.1 3,251,808 - -4.1 3,251,856 - -1.3 3,251,856 - -1.3 3,251,885 - -0.9 3,251,968 - SO3124 0.22 +0.4 3,252,060 + SO3124 0.42 -0.5 3,252,189 - SO3124 0.69 +0.4 3,252,196 + SO3124 0.70 -0.3 3,252,229 - SO3124 0.77 +1.6 3,252,239 - SO3124 0.80 -0.6 3,252,287 + SO3124 0.90 +0.1 3,252,445 - -0.6 3,252,469 + -1.2 3,252,513 - +1.4 3,252,671 + SO3125 0.21 -1.0 3,252,815 + SO3125 0.32 -1.9 3,252,833 + SO3125 0.34 -0.3 3,252,836 + SO3125 0.34 -2.3 3,253,121 + SO3125 0.56 -2.7 3,253,139 - SO3125 0.57 -2.0 3,253,338 + SO3125 0.73 +2.0 3,253,440 - SO3125 0.81 +1.2 3,253,456 - SO3125 0.82 -2.6 3,253,582 - -0.6 3,253,582 - -2.1 3,253,589 + -1.3 3,253,589 + +0.4 3,253,886 + ogt SO3126 0.33 +1.0 3,253,897 + ogt SO3126 0.35 -1.9 3,254,038 + ogt SO3126 0.63 -0.7 3,254,230 - -1.3 3,254,456 + -0.6 3,254,458 + -0.4 3,254,519 + SO3127 0.12 -1.7 3,254,566 + SO3127 0.14 +1.3 3,254,566 + SO3127 0.14 -0.7 3,254,566 + SO3127 0.14 -1.0 3,254,613 + SO3127 0.17 -1.3 3,254,670 + SO3127 0.21 -0.1
Or see this region's nucleotide sequence