Experiment: LB; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO2528 and SO2529 are separated by 788 nucleotides SO2529 and def-3 are separated by 213 nucleotides def-3 and SO2532 are separated by 293 nucleotides
SO2528: SO2528 - site-specific recombinase, phage integrase family (NCBI ptt file), at 2,657,435 to 2,658,298
SO2528
SO2529: SO2529 - hypothetical protein (NCBI ptt file), at 2,659,087 to 2,659,935
SO2529
SO2530: def-3 - polypeptide deformylase (NCBI ptt file), at 2,660,149 to 2,660,640
def-3
SO2532: SO2532 - methylated-DNA--protein-cysteine methyltransferase, putative (NCBI ptt file), at 2,660,934 to 2,661,293
SO2532
Position (kb)
2659
2660 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2658.606 kb on - strand at 2658.661 kb on - strand at 2658.749 kb on + strand at 2658.854 kb on + strand at 2658.867 kb on - strand at 2658.941 kb on + strand at 2659.305 kb on + strand, within SO2529 at 2659.311 kb on - strand, within SO2529 at 2659.313 kb on - strand, within SO2529 at 2659.319 kb on - strand, within SO2529 at 2659.369 kb on - strand, within SO2529 at 2659.369 kb on - strand, within SO2529 at 2659.807 kb on - strand, within SO2529 at 2659.954 kb on - strand at 2659.968 kb on - strand at 2660.214 kb on + strand, within def-3 at 2660.288 kb on + strand, within def-3 at 2660.302 kb on + strand, within def-3 at 2660.302 kb on + strand, within def-3 at 2660.310 kb on - strand, within def-3 at 2660.310 kb on - strand, within def-3 at 2660.310 kb on - strand, within def-3 at 2660.310 kb on - strand, within def-3 at 2660.310 kb on - strand, within def-3 at 2660.310 kb on - strand, within def-3 at 2660.350 kb on + strand, within def-3 at 2660.477 kb on + strand, within def-3 at 2660.477 kb on + strand, within def-3 at 2660.532 kb on - strand, within def-3 at 2660.532 kb on - strand, within def-3 at 2660.581 kb on + strand, within def-3 at 2660.680 kb on + strand at 2660.817 kb on - strand at 2660.933 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB; anaerobic remove 2,658,606 - -1.2 2,658,661 - +1.6 2,658,749 + -0.8 2,658,854 + -0.5 2,658,867 - +0.3 2,658,941 + -1.1 2,659,305 + SO2529 0.26 -0.4 2,659,311 - SO2529 0.26 +0.4 2,659,313 - SO2529 0.27 +1.1 2,659,319 - SO2529 0.27 -1.8 2,659,369 - SO2529 0.33 +0.1 2,659,369 - SO2529 0.33 +0.3 2,659,807 - SO2529 0.85 +3.0 2,659,954 - -2.5 2,659,968 - +0.6 2,660,214 + def-3 SO2530 0.13 -0.8 2,660,288 + def-3 SO2530 0.28 -2.8 2,660,302 + def-3 SO2530 0.31 -2.2 2,660,302 + def-3 SO2530 0.31 -0.3 2,660,310 - def-3 SO2530 0.33 +1.2 2,660,310 - def-3 SO2530 0.33 +0.8 2,660,310 - def-3 SO2530 0.33 -1.1 2,660,310 - def-3 SO2530 0.33 -0.0 2,660,310 - def-3 SO2530 0.33 +0.2 2,660,310 - def-3 SO2530 0.33 -1.4 2,660,350 + def-3 SO2530 0.41 -1.4 2,660,477 + def-3 SO2530 0.67 -0.2 2,660,477 + def-3 SO2530 0.67 -1.7 2,660,532 - def-3 SO2530 0.78 +0.2 2,660,532 - def-3 SO2530 0.78 -1.0 2,660,581 + def-3 SO2530 0.88 -2.9 2,660,680 + +0.2 2,660,817 - -2.3 2,660,933 - -0.1
Or see this region's nucleotide sequence