Experiment: LB; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt deoA and deoB are separated by 9 nucleotides deoB and deoD-2 are separated by 91 nucleotides deoD-2 and SO1222 are separated by 167 nucleotides
SO1218: deoA - thymidine phosphorylase (NCBI ptt file), at 1,264,751 to 1,266,082
deoA
SO1219: deoB - Phosphopentomutase (EC 5.4.2.7) (from data) , at 1,266,092 to 1,267,306
deoB
SO1221: deoD-2 - purine nucleoside phosphorylase (NCBI ptt file), at 1,267,398 to 1,268,108
deoD-2
SO1222: SO1222 - hypothetical protein (NCBI ptt file), at 1,268,276 to 1,269,499
SO1222
Position (kb)
1266
1267
1268 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1265.222 kb on + strand, within deoA at 1265.279 kb on - strand, within deoA at 1265.304 kb on + strand, within deoA at 1265.386 kb on + strand, within deoA at 1265.400 kb on + strand, within deoA at 1265.405 kb on - strand, within deoA at 1265.459 kb on + strand, within deoA at 1265.467 kb on - strand, within deoA at 1265.488 kb on + strand, within deoA at 1265.559 kb on - strand, within deoA at 1265.563 kb on + strand, within deoA at 1265.577 kb on + strand, within deoA at 1265.577 kb on + strand, within deoA at 1265.585 kb on - strand, within deoA at 1265.808 kb on + strand, within deoA at 1265.817 kb on + strand, within deoA at 1265.868 kb on - strand, within deoA at 1265.889 kb on + strand, within deoA at 1265.889 kb on - strand, within deoA at 1265.894 kb on + strand, within deoA at 1265.897 kb on - strand, within deoA at 1266.077 kb on - strand at 1266.123 kb on + strand at 1266.123 kb on + strand at 1266.269 kb on + strand, within deoB at 1266.269 kb on + strand, within deoB at 1266.269 kb on + strand, within deoB at 1266.277 kb on - strand, within deoB at 1266.485 kb on + strand, within deoB at 1266.495 kb on + strand, within deoB at 1266.554 kb on + strand, within deoB at 1266.706 kb on - strand, within deoB at 1266.876 kb on - strand, within deoB at 1266.876 kb on - strand, within deoB at 1266.912 kb on + strand, within deoB at 1267.025 kb on - strand, within deoB at 1267.093 kb on - strand, within deoB at 1267.139 kb on + strand, within deoB at 1267.149 kb on + strand, within deoB at 1267.157 kb on - strand, within deoB at 1267.157 kb on - strand, within deoB at 1267.186 kb on - strand at 1267.346 kb on - strand at 1267.346 kb on - strand at 1267.346 kb on - strand at 1267.346 kb on - strand at 1267.412 kb on + strand at 1267.479 kb on - strand, within deoD-2 at 1267.573 kb on - strand, within deoD-2 at 1267.687 kb on - strand, within deoD-2 at 1267.699 kb on - strand, within deoD-2 at 1267.724 kb on + strand, within deoD-2 at 1267.732 kb on - strand, within deoD-2 at 1267.807 kb on - strand, within deoD-2 at 1267.965 kb on + strand, within deoD-2 at 1268.038 kb on + strand at 1268.105 kb on + strand at 1268.113 kb on - strand at 1268.116 kb on + strand at 1268.116 kb on + strand at 1268.116 kb on + strand at 1268.116 kb on + strand at 1268.116 kb on + strand at 1268.124 kb on - strand at 1268.124 kb on - strand at 1268.124 kb on - strand at 1268.124 kb on - strand at 1268.124 kb on - strand at 1268.124 kb on - strand at 1268.124 kb on - strand at 1268.124 kb on - strand at 1268.124 kb on - strand at 1268.124 kb on - strand at 1268.124 kb on - strand at 1268.124 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB; anaerobic remove 1,265,222 + deoA SO1218 0.35 -0.7 1,265,279 - deoA SO1218 0.40 -0.7 1,265,304 + deoA SO1218 0.42 -0.4 1,265,386 + deoA SO1218 0.48 -3.3 1,265,400 + deoA SO1218 0.49 -2.7 1,265,405 - deoA SO1218 0.49 -2.5 1,265,459 + deoA SO1218 0.53 -2.7 1,265,467 - deoA SO1218 0.54 +0.2 1,265,488 + deoA SO1218 0.55 -0.1 1,265,559 - deoA SO1218 0.61 +0.2 1,265,563 + deoA SO1218 0.61 +0.2 1,265,577 + deoA SO1218 0.62 +0.0 1,265,577 + deoA SO1218 0.62 -1.8 1,265,585 - deoA SO1218 0.63 -0.5 1,265,808 + deoA SO1218 0.79 -0.0 1,265,817 + deoA SO1218 0.80 -0.2 1,265,868 - deoA SO1218 0.84 -0.6 1,265,889 + deoA SO1218 0.85 -2.5 1,265,889 - deoA SO1218 0.85 -1.6 1,265,894 + deoA SO1218 0.86 -0.7 1,265,897 - deoA SO1218 0.86 +0.1 1,266,077 - +0.2 1,266,123 + -0.7 1,266,123 + -0.1 1,266,269 + deoB SO1219 0.15 -0.9 1,266,269 + deoB SO1219 0.15 -1.4 1,266,269 + deoB SO1219 0.15 -1.5 1,266,277 - deoB SO1219 0.15 +0.1 1,266,485 + deoB SO1219 0.32 +0.4 1,266,495 + deoB SO1219 0.33 -2.0 1,266,554 + deoB SO1219 0.38 -2.8 1,266,706 - deoB SO1219 0.51 -1.5 1,266,876 - deoB SO1219 0.65 -2.1 1,266,876 - deoB SO1219 0.65 +0.1 1,266,912 + deoB SO1219 0.67 +0.5 1,267,025 - deoB SO1219 0.77 -3.1 1,267,093 - deoB SO1219 0.82 +1.9 1,267,139 + deoB SO1219 0.86 -1.5 1,267,149 + deoB SO1219 0.87 +0.3 1,267,157 - deoB SO1219 0.88 -2.1 1,267,157 - deoB SO1219 0.88 -0.1 1,267,186 - -0.6 1,267,346 - -1.5 1,267,346 - -0.5 1,267,346 - -1.3 1,267,346 - -1.1 1,267,412 + -1.4 1,267,479 - deoD-2 SO1221 0.11 -0.1 1,267,573 - deoD-2 SO1221 0.25 +0.3 1,267,687 - deoD-2 SO1221 0.41 -0.4 1,267,699 - deoD-2 SO1221 0.42 -1.5 1,267,724 + deoD-2 SO1221 0.46 +0.1 1,267,732 - deoD-2 SO1221 0.47 -1.4 1,267,807 - deoD-2 SO1221 0.58 -2.3 1,267,965 + deoD-2 SO1221 0.80 +0.6 1,268,038 + -1.8 1,268,105 + -0.9 1,268,113 - +0.4 1,268,116 + -1.6 1,268,116 + +0.3 1,268,116 + -0.1 1,268,116 + +1.0 1,268,116 + -1.7 1,268,124 - +1.7 1,268,124 - +1.3 1,268,124 - +1.3 1,268,124 - +0.4 1,268,124 - -1.4 1,268,124 - +1.1 1,268,124 - -0.2 1,268,124 - -1.2 1,268,124 - -0.4 1,268,124 - -0.7 1,268,124 - -2.3 1,268,124 - -0.4
Or see this region's nucleotide sequence