Strain Fitness in Shewanella oneidensis MR-1 around SO3465

Experiment: fumarate electron acceptor

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntthiL and nusB are separated by 78 nucleotidesnusB and ribH are separated by 14 nucleotidesribH and ribBA are separated by 140 nucleotides SO3464: thiL - thiamin-monophosphate kinase (NCBI ptt file), at 3,610,267 to 3,611,223 thiL SO3465: nusB - N utilization substance protein B (NCBI ptt file), at 3,611,302 to 3,611,706 nusB SO3466: ribH - riboflavin synthase, beta subunit (NCBI ptt file), at 3,611,721 to 3,612,197 ribH SO3467: ribBA - 3,4-dihydroxy-2-butanone 4-phosphate synthase/GTP cyclohydrolase II, putative (NCBI ptt file), at 3,612,338 to 3,613,441 ribBA Position (kb) 3611 3612Strain fitness (log2 ratio) -3 -2 -1 0 1at 3612.327 kb on + strandat 3612.369 kb on - strandat 3612.374 kb on + strandat 3612.374 kb on + strandat 3612.382 kb on - strandat 3612.382 kb on - strandat 3612.452 kb on + strand, within ribBAat 3612.457 kb on + strand, within ribBAat 3612.465 kb on - strand, within ribBAat 3612.465 kb on - strand, within ribBAat 3612.500 kb on + strand, within ribBAat 3612.508 kb on - strand, within ribBAat 3612.590 kb on + strand, within ribBAat 3612.590 kb on + strand, within ribBAat 3612.675 kb on - strand, within ribBA

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Per-strain Table

Position Strand Gene LocusTag Fraction fumarate electron acceptor
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3,612,327 + -0.6
3,612,369 - +1.7
3,612,374 + -0.2
3,612,374 + +0.2
3,612,382 - -0.7
3,612,382 - -0.0
3,612,452 + ribBA SO3467 0.10 -1.0
3,612,457 + ribBA SO3467 0.11 -3.3
3,612,465 - ribBA SO3467 0.12 +0.1
3,612,465 - ribBA SO3467 0.12 +0.3
3,612,500 + ribBA SO3467 0.15 -1.1
3,612,508 - ribBA SO3467 0.15 -0.2
3,612,590 + ribBA SO3467 0.23 -0.7
3,612,590 + ribBA SO3467 0.23 +0.9
3,612,675 - ribBA SO3467 0.31 +0.3

Or see this region's nucleotide sequence