Experiment: fumarate electron acceptor
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mshL and mshK are separated by 14 nucleotides mshK and mshJ overlap by 14 nucleotides mshJ and SO_4115 overlap by 4 nucleotides SO_4115 and SO4114.2 are separated by 71 nucleotides
SO4112: mshL - MSHA biogenesis protein MshL (NCBI ptt file), at 4,270,814 to 4,272,496
mshL
SO4113: mshK - MSHA biogenesis protein MshK (NCBI ptt file), at 4,272,511 to 4,272,828
mshK
SO4114: mshJ - MSHA biogenesis protein MshJ (NCBI ptt file), at 4,272,815 to 4,273,465
mshJ
SO_4115: SO_4115 - no description, at 4,273,462 to 4,273,989
_4115
SO4114.2: SO4114.2 - hypothetical biogenesis protein MshI (NCBI ptt file), at 4,274,061 to 4,274,939
SO4114.2
Position (kb)
4273
4274 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 4272.471 kb on + strand at 4272.472 kb on - strand at 4272.535 kb on - strand at 4272.590 kb on + strand, within mshK at 4272.598 kb on - strand, within mshK at 4272.615 kb on - strand, within mshK at 4272.701 kb on + strand, within mshK at 4272.906 kb on - strand, within mshJ at 4272.941 kb on - strand, within mshJ at 4272.979 kb on - strand, within mshJ at 4273.073 kb on - strand, within mshJ at 4273.129 kb on - strand, within mshJ at 4273.218 kb on + strand, within mshJ at 4273.218 kb on + strand, within mshJ at 4273.320 kb on + strand, within mshJ at 4273.474 kb on + strand at 4273.474 kb on + strand at 4273.474 kb on + strand at 4273.474 kb on + strand at 4273.476 kb on + strand at 4273.482 kb on - strand at 4273.484 kb on - strand at 4273.713 kb on + strand, within SO_4115 at 4273.721 kb on - strand, within SO_4115 at 4273.728 kb on - strand, within SO_4115 at 4273.742 kb on - strand, within SO_4115 at 4273.785 kb on + strand, within SO_4115 at 4273.793 kb on - strand, within SO_4115 at 4273.799 kb on - strand, within SO_4115 at 4273.804 kb on - strand, within SO_4115 at 4273.826 kb on + strand, within SO_4115 at 4273.826 kb on + strand, within SO_4115 at 4273.826 kb on + strand, within SO_4115 at 4273.826 kb on + strand, within SO_4115 at 4273.834 kb on - strand, within SO_4115 at 4273.834 kb on - strand, within SO_4115 at 4273.834 kb on - strand, within SO_4115 at 4273.834 kb on - strand, within SO_4115 at 4273.911 kb on + strand, within SO_4115 at 4274.098 kb on - strand at 4274.099 kb on - strand at 4274.166 kb on - strand, within SO4114.2 at 4274.178 kb on + strand, within SO4114.2 at 4274.214 kb on + strand, within SO4114.2 at 4274.239 kb on + strand, within SO4114.2 at 4274.319 kb on - strand, within SO4114.2 at 4274.330 kb on + strand, within SO4114.2 at 4274.338 kb on - strand, within SO4114.2 at 4274.338 kb on - strand, within SO4114.2 at 4274.338 kb on - strand, within SO4114.2 at 4274.381 kb on - strand, within SO4114.2 at 4274.425 kb on - strand, within SO4114.2 at 4274.468 kb on + strand, within SO4114.2 at 4274.468 kb on + strand, within SO4114.2 at 4274.468 kb on + strand, within SO4114.2 at 4274.468 kb on + strand, within SO4114.2 at 4274.468 kb on + strand, within SO4114.2 at 4274.468 kb on + strand, within SO4114.2 at 4274.476 kb on - strand, within SO4114.2 at 4274.476 kb on - strand, within SO4114.2 at 4274.476 kb on - strand, within SO4114.2 at 4274.476 kb on - strand, within SO4114.2 at 4274.504 kb on + strand, within SO4114.2 at 4274.561 kb on + strand, within SO4114.2 at 4274.562 kb on - strand, within SO4114.2 at 4274.566 kb on + strand, within SO4114.2 at 4274.571 kb on + strand, within SO4114.2 at 4274.579 kb on - strand, within SO4114.2 at 4274.636 kb on - strand, within SO4114.2 at 4274.651 kb on - strand, within SO4114.2 at 4274.651 kb on - strand, within SO4114.2 at 4274.747 kb on - strand, within SO4114.2 at 4274.852 kb on - strand at 4274.853 kb on - strand at 4274.873 kb on - strand at 4274.879 kb on + strand at 4274.879 kb on + strand at 4274.879 kb on + strand at 4274.879 kb on + strand at 4274.970 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction fumarate electron acceptor remove 4,272,471 + -2.1 4,272,472 - -1.5 4,272,535 - -0.9 4,272,590 + mshK SO4113 0.25 +0.3 4,272,598 - mshK SO4113 0.27 -3.6 4,272,615 - mshK SO4113 0.33 -2.2 4,272,701 + mshK SO4113 0.60 -2.4 4,272,906 - mshJ SO4114 0.14 -1.9 4,272,941 - mshJ SO4114 0.19 -5.0 4,272,979 - mshJ SO4114 0.25 -0.8 4,273,073 - mshJ SO4114 0.40 -1.8 4,273,129 - mshJ SO4114 0.48 -1.8 4,273,218 + mshJ SO4114 0.62 -1.8 4,273,218 + mshJ SO4114 0.62 -4.0 4,273,320 + mshJ SO4114 0.78 -1.7 4,273,474 + -2.5 4,273,474 + -1.9 4,273,474 + -3.5 4,273,474 + -4.7 4,273,476 + -0.9 4,273,482 - -1.1 4,273,484 - -0.3 4,273,713 + SO_4115 0.48 -3.2 4,273,721 - SO_4115 0.49 -4.4 4,273,728 - SO_4115 0.50 -2.8 4,273,742 - SO_4115 0.53 -1.6 4,273,785 + SO_4115 0.61 -1.9 4,273,793 - SO_4115 0.63 -1.4 4,273,799 - SO_4115 0.64 +1.8 4,273,804 - SO_4115 0.65 -0.7 4,273,826 + SO_4115 0.69 -1.5 4,273,826 + SO_4115 0.69 -3.3 4,273,826 + SO_4115 0.69 -0.7 4,273,826 + SO_4115 0.69 -0.7 4,273,834 - SO_4115 0.70 -3.1 4,273,834 - SO_4115 0.70 +0.9 4,273,834 - SO_4115 0.70 -1.6 4,273,834 - SO_4115 0.70 -2.2 4,273,911 + SO_4115 0.85 -2.2 4,274,098 - +0.3 4,274,099 - -0.9 4,274,166 - SO4114.2 0.12 -2.7 4,274,178 + SO4114.2 0.13 -1.3 4,274,214 + SO4114.2 0.17 -3.9 4,274,239 + SO4114.2 0.20 -1.8 4,274,319 - SO4114.2 0.29 -2.9 4,274,330 + SO4114.2 0.31 -1.6 4,274,338 - SO4114.2 0.32 -3.1 4,274,338 - SO4114.2 0.32 -3.2 4,274,338 - SO4114.2 0.32 -3.6 4,274,381 - SO4114.2 0.36 -1.8 4,274,425 - SO4114.2 0.41 -3.9 4,274,468 + SO4114.2 0.46 -1.5 4,274,468 + SO4114.2 0.46 -1.6 4,274,468 + SO4114.2 0.46 +0.5 4,274,468 + SO4114.2 0.46 -1.6 4,274,468 + SO4114.2 0.46 +0.1 4,274,468 + SO4114.2 0.46 -2.1 4,274,476 - SO4114.2 0.47 -1.5 4,274,476 - SO4114.2 0.47 -0.9 4,274,476 - SO4114.2 0.47 +0.6 4,274,476 - SO4114.2 0.47 -1.4 4,274,504 + SO4114.2 0.50 -0.8 4,274,561 + SO4114.2 0.57 -0.1 4,274,562 - SO4114.2 0.57 -3.8 4,274,566 + SO4114.2 0.57 -1.8 4,274,571 + SO4114.2 0.58 -2.1 4,274,579 - SO4114.2 0.59 -2.2 4,274,636 - SO4114.2 0.65 -3.0 4,274,651 - SO4114.2 0.67 -2.6 4,274,651 - SO4114.2 0.67 -3.6 4,274,747 - SO4114.2 0.78 -1.5 4,274,852 - +0.1 4,274,853 - -2.0 4,274,873 - -2.8 4,274,879 + -4.9 4,274,879 + -1.4 4,274,879 + +0.3 4,274,879 + -1.1 4,274,970 - +0.6
Or see this region's nucleotide sequence