Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS01865

Experiment: 2-Deoxy-D-ribonic lithium salt 10 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS01855 and BWI76_RS01860 are separated by 12 nucleotidesBWI76_RS01860 and BWI76_RS01865 are separated by 98 nucleotidesBWI76_RS01865 and BWI76_RS01870 are separated by 129 nucleotidesBWI76_RS01870 and BWI76_RS01875 are separated by 122 nucleotides BWI76_RS01855: BWI76_RS01855 - chorismate lyase, at 378,583 to 379,080 _RS01855 BWI76_RS01860: BWI76_RS01860 - 4-hydroxybenzoate octaprenyltransferase, at 379,093 to 379,962 _RS01860 BWI76_RS01865: BWI76_RS01865 - glycerol-3-phosphate 1-O-acyltransferase, at 380,061 to 382,484 _RS01865 BWI76_RS01870: BWI76_RS01870 - diacylglycerol kinase, at 382,614 to 382,982 _RS01870 BWI76_RS01875: BWI76_RS01875 - repressor LexA, at 383,105 to 383,713 _RS01875 Position (kb) 380 381 382 383Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 379.069 kb on + strandat 380.039 kb on + strandat 382.488 kb on - strandat 382.536 kb on - strandat 382.571 kb on + strandat 382.571 kb on + strandat 382.571 kb on + strandat 382.627 kb on + strandat 382.764 kb on - strand, within BWI76_RS01870at 382.798 kb on - strand, within BWI76_RS01870at 382.803 kb on - strand, within BWI76_RS01870at 382.873 kb on + strand, within BWI76_RS01870at 382.907 kb on - strand, within BWI76_RS01870at 382.995 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxy-D-ribonic lithium salt 10 mM (C)
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379,069 + +0.8
380,039 + +0.0
382,488 - -3.0
382,536 - -2.2
382,571 + -0.6
382,571 + +0.3
382,571 + -0.7
382,627 + -1.7
382,764 - BWI76_RS01870 0.41 -0.3
382,798 - BWI76_RS01870 0.50 -0.8
382,803 - BWI76_RS01870 0.51 +0.8
382,873 + BWI76_RS01870 0.70 +1.3
382,907 - BWI76_RS01870 0.79 +2.2
382,995 - +0.6

Or see this region's nucleotide sequence