Experiment: biofilm; planktonic cells from 96 well plate assay in R2A with C30 compound
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE53_000127 and ABIE53_000128 are separated by 19 nucleotides ABIE53_000128 and ABIE53_000129 are separated by 243 nucleotides ABIE53_000129 and ABIE53_000130 are separated by 49 nucleotides
ABIE53_000127: ABIE53_000127 - chemotaxis protein MotB, at 134,034 to 135,041
_000127
ABIE53_000128: ABIE53_000128 - chemotaxis protein MotA, at 135,061 to 135,921
_000128
ABIE53_000129: ABIE53_000129 - flagellar transcriptional activator FlhC, at 136,165 to 136,725
_000129
ABIE53_000130: ABIE53_000130 - flagellar transcriptional activator FlhD, at 136,775 to 137,095
_000130
Position (kb) 135
136 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6
7
8 at 134.360 kb on - strand, within ABIE53_000127 at 134.360 kb on - strand, within ABIE53_000127 at 134.380 kb on + strand, within ABIE53_000127 at 134.381 kb on - strand, within ABIE53_000127 at 134.381 kb on - strand, within ABIE53_000127 at 134.470 kb on + strand, within ABIE53_000127 at 134.470 kb on + strand, within ABIE53_000127 at 134.470 kb on + strand, within ABIE53_000127 at 134.470 kb on + strand, within ABIE53_000127 at 134.470 kb on + strand, within ABIE53_000127 at 134.470 kb on + strand, within ABIE53_000127 at 134.470 kb on + strand, within ABIE53_000127 at 134.470 kb on + strand, within ABIE53_000127 at 134.470 kb on + strand, within ABIE53_000127 at 134.470 kb on + strand, within ABIE53_000127 at 134.470 kb on + strand, within ABIE53_000127 at 134.471 kb on - strand, within ABIE53_000127 at 134.774 kb on + strand, within ABIE53_000127 at 134.774 kb on + strand, within ABIE53_000127 at 134.775 kb on - strand, within ABIE53_000127 at 134.775 kb on - strand, within ABIE53_000127 at 134.775 kb on - strand, within ABIE53_000127 at 134.861 kb on - strand, within ABIE53_000127 at 134.861 kb on - strand, within ABIE53_000127 at 134.861 kb on - strand, within ABIE53_000127 at 134.861 kb on - strand, within ABIE53_000127 at 135.062 kb on + strand at 135.062 kb on + strand at 135.062 kb on + strand at 135.062 kb on + strand at 135.062 kb on + strand at 135.062 kb on + strand at 135.062 kb on + strand at 135.063 kb on - strand at 135.063 kb on - strand at 135.063 kb on - strand at 135.063 kb on - strand at 135.167 kb on + strand, within ABIE53_000128 at 135.167 kb on + strand, within ABIE53_000128 at 135.272 kb on + strand, within ABIE53_000128 at 135.272 kb on + strand, within ABIE53_000128 at 135.272 kb on + strand, within ABIE53_000128 at 135.273 kb on - strand, within ABIE53_000128 at 135.536 kb on + strand, within ABIE53_000128 at 135.536 kb on + strand, within ABIE53_000128 at 135.536 kb on + strand, within ABIE53_000128 at 135.536 kb on + strand, within ABIE53_000128 at 135.537 kb on - strand, within ABIE53_000128 at 135.537 kb on - strand, within ABIE53_000128 at 135.537 kb on - strand, within ABIE53_000128 at 135.585 kb on - strand, within ABIE53_000128 at 135.674 kb on + strand, within ABIE53_000128 at 135.674 kb on + strand, within ABIE53_000128 at 135.674 kb on + strand, within ABIE53_000128 at 135.674 kb on + strand, within ABIE53_000128 at 135.675 kb on - strand, within ABIE53_000128 at 135.675 kb on - strand, within ABIE53_000128 at 135.675 kb on - strand, within ABIE53_000128 at 135.675 kb on - strand, within ABIE53_000128 at 135.675 kb on - strand, within ABIE53_000128 at 135.698 kb on + strand, within ABIE53_000128 at 135.698 kb on + strand, within ABIE53_000128 at 135.698 kb on + strand, within ABIE53_000128 at 135.698 kb on + strand, within ABIE53_000128 at 135.698 kb on + strand, within ABIE53_000128 at 135.699 kb on - strand, within ABIE53_000128 at 135.699 kb on - strand, within ABIE53_000128 at 135.699 kb on - strand, within ABIE53_000128 at 135.699 kb on - strand, within ABIE53_000128 at 135.699 kb on - strand, within ABIE53_000128 at 135.699 kb on - strand, within ABIE53_000128 at 135.699 kb on - strand, within ABIE53_000128 at 135.699 kb on - strand, within ABIE53_000128 at 135.699 kb on - strand, within ABIE53_000128 at 135.713 kb on + strand, within ABIE53_000128 at 135.713 kb on + strand, within ABIE53_000128 at 135.714 kb on - strand, within ABIE53_000128 at 135.714 kb on - strand, within ABIE53_000128 at 135.714 kb on - strand, within ABIE53_000128 at 135.714 kb on - strand, within ABIE53_000128 at 135.787 kb on - strand, within ABIE53_000128 at 135.959 kb on + strand at 135.960 kb on - strand at 136.021 kb on + strand at 136.021 kb on + strand at 136.021 kb on + strand at 136.021 kb on + strand at 136.120 kb on + strand at 136.120 kb on + strand at 136.120 kb on + strand at 136.121 kb on - strand at 136.166 kb on + strand at 136.225 kb on - strand, within ABIE53_000129 at 136.421 kb on + strand, within ABIE53_000129 at 136.422 kb on - strand, within ABIE53_000129 at 136.430 kb on + strand, within ABIE53_000129 at 136.487 kb on + strand, within ABIE53_000129 at 136.488 kb on + strand, within ABIE53_000129 at 136.488 kb on - strand, within ABIE53_000129 at 136.488 kb on - strand, within ABIE53_000129 at 136.488 kb on - strand, within ABIE53_000129 at 136.488 kb on - strand, within ABIE53_000129 at 136.496 kb on + strand, within ABIE53_000129 at 136.496 kb on + strand, within ABIE53_000129 at 136.496 kb on + strand, within ABIE53_000129 at 136.496 kb on + strand, within ABIE53_000129 at 136.496 kb on + strand, within ABIE53_000129 at 136.496 kb on + strand, within ABIE53_000129 at 136.497 kb on - strand, within ABIE53_000129 at 136.497 kb on - strand, within ABIE53_000129 at 136.497 kb on - strand, within ABIE53_000129 at 136.497 kb on - strand, within ABIE53_000129 at 136.497 kb on - strand, within ABIE53_000129 at 136.595 kb on + strand, within ABIE53_000129 at 136.595 kb on + strand, within ABIE53_000129 at 136.595 kb on + strand, within ABIE53_000129 at 136.595 kb on + strand, within ABIE53_000129 at 136.595 kb on + strand, within ABIE53_000129 at 136.595 kb on + strand, within ABIE53_000129 at 136.595 kb on + strand, within ABIE53_000129 at 136.595 kb on + strand, within ABIE53_000129 at 136.596 kb on - strand, within ABIE53_000129 at 136.596 kb on - strand, within ABIE53_000129 at 136.596 kb on - strand, within ABIE53_000129 at 136.596 kb on - strand, within ABIE53_000129 at 136.596 kb on - strand, within ABIE53_000129 at 136.596 kb on - strand, within ABIE53_000129 at 136.596 kb on - strand, within ABIE53_000129 at 136.716 kb on + strand at 136.716 kb on + strand at 136.716 kb on + strand at 136.716 kb on + strand at 136.716 kb on + strand at 136.717 kb on - strand at 136.717 kb on - strand at 136.717 kb on - strand at 136.717 kb on - strand at 136.717 kb on - strand at 136.717 kb on - strand at 136.717 kb on - strand at 136.717 kb on - strand at 136.717 kb on - strand at 136.717 kb on - strand at 136.717 kb on - strand at 136.717 kb on - strand at 136.717 kb on - strand at 136.717 kb on - strand at 136.717 kb on - strand at 136.718 kb on + strand at 136.718 kb on + strand at 136.718 kb on + strand at 136.718 kb on + strand at 136.718 kb on + strand at 136.718 kb on + strand at 136.718 kb on + strand at 136.719 kb on - strand at 136.719 kb on - strand at 136.719 kb on - strand at 136.719 kb on - strand at 136.719 kb on - strand at 136.719 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction biofilm; planktonic cells from 96 well plate assay in R2A with C30 compound remove 134,360 - ABIE53_000127 0.32 +4.9 134,360 - ABIE53_000127 0.32 +4.2 134,380 + ABIE53_000127 0.34 +7.7 134,381 - ABIE53_000127 0.34 +4.3 134,381 - ABIE53_000127 0.34 +4.1 134,470 + ABIE53_000127 0.43 +4.9 134,470 + ABIE53_000127 0.43 +4.4 134,470 + ABIE53_000127 0.43 +5.7 134,470 + ABIE53_000127 0.43 +5.7 134,470 + ABIE53_000127 0.43 +6.0 134,470 + ABIE53_000127 0.43 +7.0 134,470 + ABIE53_000127 0.43 +6.3 134,470 + ABIE53_000127 0.43 +7.0 134,470 + ABIE53_000127 0.43 +2.9 134,470 + ABIE53_000127 0.43 +6.4 134,470 + ABIE53_000127 0.43 +6.2 134,471 - ABIE53_000127 0.43 +4.5 134,774 + ABIE53_000127 0.73 +4.7 134,774 + ABIE53_000127 0.73 +6.2 134,775 - ABIE53_000127 0.74 +4.9 134,775 - ABIE53_000127 0.74 +3.2 134,775 - ABIE53_000127 0.74 +5.0 134,861 - ABIE53_000127 0.82 +5.3 134,861 - ABIE53_000127 0.82 +7.6 134,861 - ABIE53_000127 0.82 +4.3 134,861 - ABIE53_000127 0.82 +4.7 135,062 + +4.7 135,062 + +6.4 135,062 + +6.0 135,062 + +4.9 135,062 + +7.1 135,062 + +6.1 135,062 + +4.3 135,063 - -1.4 135,063 - -3.2 135,063 - +1.1 135,063 - +1.9 135,167 + ABIE53_000128 0.12 +6.7 135,167 + ABIE53_000128 0.12 +5.8 135,272 + ABIE53_000128 0.25 +6.7 135,272 + ABIE53_000128 0.25 +5.2 135,272 + ABIE53_000128 0.25 +5.7 135,273 - ABIE53_000128 0.25 +6.6 135,536 + ABIE53_000128 0.55 +7.8 135,536 + ABIE53_000128 0.55 +6.6 135,536 + ABIE53_000128 0.55 +6.4 135,536 + ABIE53_000128 0.55 +5.6 135,537 - ABIE53_000128 0.55 +5.2 135,537 - ABIE53_000128 0.55 +5.2 135,537 - ABIE53_000128 0.55 +4.2 135,585 - ABIE53_000128 0.61 +5.5 135,674 + ABIE53_000128 0.71 +6.3 135,674 + ABIE53_000128 0.71 +6.4 135,674 + ABIE53_000128 0.71 +6.9 135,674 + ABIE53_000128 0.71 +4.7 135,675 - ABIE53_000128 0.71 +4.7 135,675 - ABIE53_000128 0.71 +5.5 135,675 - ABIE53_000128 0.71 +5.2 135,675 - ABIE53_000128 0.71 +5.7 135,675 - ABIE53_000128 0.71 +5.1 135,698 + ABIE53_000128 0.74 +5.8 135,698 + ABIE53_000128 0.74 +3.9 135,698 + ABIE53_000128 0.74 +6.7 135,698 + ABIE53_000128 0.74 +6.0 135,698 + ABIE53_000128 0.74 +6.6 135,699 - ABIE53_000128 0.74 +4.9 135,699 - ABIE53_000128 0.74 +3.5 135,699 - ABIE53_000128 0.74 +4.7 135,699 - ABIE53_000128 0.74 +4.9 135,699 - ABIE53_000128 0.74 +4.0 135,699 - ABIE53_000128 0.74 +4.7 135,699 - ABIE53_000128 0.74 +6.0 135,699 - ABIE53_000128 0.74 +5.5 135,699 - ABIE53_000128 0.74 +5.1 135,713 + ABIE53_000128 0.76 +6.7 135,713 + ABIE53_000128 0.76 +6.4 135,714 - ABIE53_000128 0.76 +4.0 135,714 - ABIE53_000128 0.76 +4.7 135,714 - ABIE53_000128 0.76 +5.4 135,714 - ABIE53_000128 0.76 +3.3 135,787 - ABIE53_000128 0.84 +4.7 135,959 + +7.1 135,960 - -0.8 136,021 + +1.6 136,021 + -0.8 136,021 + -0.3 136,021 + +2.2 136,120 + -0.5 136,120 + -0.5 136,120 + -0.4 136,121 - -1.2 136,166 + +0.7 136,225 - ABIE53_000129 0.11 -0.8 136,421 + ABIE53_000129 0.46 -0.4 136,422 - ABIE53_000129 0.46 +1.3 136,430 + ABIE53_000129 0.47 +0.4 136,487 + ABIE53_000129 0.57 -0.4 136,488 + ABIE53_000129 0.58 +0.9 136,488 - ABIE53_000129 0.58 -0.4 136,488 - ABIE53_000129 0.58 +1.1 136,488 - ABIE53_000129 0.58 -1.7 136,488 - ABIE53_000129 0.58 -0.3 136,496 + ABIE53_000129 0.59 +0.5 136,496 + ABIE53_000129 0.59 +1.9 136,496 + ABIE53_000129 0.59 +0.2 136,496 + ABIE53_000129 0.59 -1.1 136,496 + ABIE53_000129 0.59 -1.5 136,496 + ABIE53_000129 0.59 +2.9 136,497 - ABIE53_000129 0.59 -0.4 136,497 - ABIE53_000129 0.59 +1.0 136,497 - ABIE53_000129 0.59 +0.1 136,497 - ABIE53_000129 0.59 -1.8 136,497 - ABIE53_000129 0.59 -1.3 136,595 + ABIE53_000129 0.77 -1.4 136,595 + ABIE53_000129 0.77 +0.3 136,595 + ABIE53_000129 0.77 -0.3 136,595 + ABIE53_000129 0.77 +0.5 136,595 + ABIE53_000129 0.77 -0.4 136,595 + ABIE53_000129 0.77 -1.1 136,595 + ABIE53_000129 0.77 -0.5 136,595 + ABIE53_000129 0.77 -0.7 136,596 - ABIE53_000129 0.77 -1.0 136,596 - ABIE53_000129 0.77 -0.7 136,596 - ABIE53_000129 0.77 -0.7 136,596 - ABIE53_000129 0.77 +1.7 136,596 - ABIE53_000129 0.77 +1.6 136,596 - ABIE53_000129 0.77 +1.3 136,596 - ABIE53_000129 0.77 +0.1 136,716 + -0.5 136,716 + +0.5 136,716 + -0.1 136,716 + -0.2 136,716 + -0.7 136,717 - -0.3 136,717 - -0.3 136,717 - +0.2 136,717 - -0.3 136,717 - -1.8 136,717 - +1.9 136,717 - +0.7 136,717 - +0.9 136,717 - +0.6 136,717 - +0.1 136,717 - -0.7 136,717 - -1.7 136,717 - +0.5 136,717 - -0.5 136,717 - -0.4 136,718 + +0.5 136,718 + +0.3 136,718 + -0.7 136,718 + +0.0 136,718 + +0.1 136,718 + -0.6 136,718 + +0.3 136,719 - +1.8 136,719 - -0.7 136,719 - +0.3 136,719 - -0.4 136,719 - -1.0 136,719 - -1.1
Or see this region's nucleotide sequence