Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS25375

Experiment: 2'-Deoxyinosine 5 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS25370 and BWI76_RS25375 are separated by 26 nucleotidesBWI76_RS25375 and BWI76_RS25380 are separated by 126 nucleotides BWI76_RS25370: BWI76_RS25370 - outer membrane-stress sensor serine endopeptidase DegS, at 5,273,383 to 5,274,441 _RS25370 BWI76_RS25375: BWI76_RS25375 - hypothetical protein, at 5,274,468 to 5,275,166 _RS25375 BWI76_RS25380: BWI76_RS25380 - oxaloacetate decarboxylase subunit beta, at 5,275,293 to 5,276,594 _RS25380 Position (kb) 5274 5275 5276Strain fitness (log2 ratio) -3 -2 -1 0 1at 5274.451 kb on + strandat 5274.490 kb on + strandat 5274.574 kb on - strand, within BWI76_RS25375at 5274.606 kb on + strandat 5274.825 kb on - strand, within BWI76_RS25375at 5275.217 kb on + strandat 5275.293 kb on - strandat 5275.946 kb on - strand, within BWI76_RS25380

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Per-strain Table

Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C)
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5,274,451 + -0.7
5,274,490 + -1.2
5,274,574 - BWI76_RS25375 0.15 -2.9
5,274,606 + +0.1
5,274,825 - BWI76_RS25375 0.51 -1.1
5,275,217 + +1.4
5,275,293 - -0.5
5,275,946 - BWI76_RS25380 0.50 +0.1

Or see this region's nucleotide sequence