Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13085 and BWI76_RS13090 are separated by 286 nucleotides BWI76_RS13090 and BWI76_RS13095 are separated by 11 nucleotides BWI76_RS13095 and BWI76_RS13100 are separated by 7 nucleotides
BWI76_RS13085: BWI76_RS13085 - bifunctional oxepin-CoA hydrolase/3-oxo-5,6-dehydrosuberyl-CoA semialdehyde dehydrogenase, at 2,729,458 to 2,731,503
_RS13085
BWI76_RS13090: BWI76_RS13090 - phenylacetate-CoA oxygenase subunit PaaA, at 2,731,790 to 2,732,719
_RS13090
BWI76_RS13095: BWI76_RS13095 - phenylacetate-CoA oxygenase subunit PaaB, at 2,732,731 to 2,733,018
_RS13095
BWI76_RS13100: BWI76_RS13100 - phenylacetate-CoA oxygenase subunit PaaI, at 2,733,026 to 2,733,781
_RS13100
Position (kb)
2731
2732
2733 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2731.077 kb on - strand, within BWI76_RS13085 at 2731.806 kb on + strand at 2731.992 kb on - strand, within BWI76_RS13090 at 2732.023 kb on - strand, within BWI76_RS13090 at 2732.123 kb on + strand, within BWI76_RS13090 at 2732.224 kb on - strand, within BWI76_RS13090 at 2732.879 kb on + strand, within BWI76_RS13095 at 2732.982 kb on + strand, within BWI76_RS13095 at 2732.990 kb on - strand at 2733.007 kb on + strand at 2733.042 kb on - strand at 2733.362 kb on + strand, within BWI76_RS13100 at 2733.402 kb on + strand, within BWI76_RS13100 at 2733.453 kb on + strand, within BWI76_RS13100 at 2733.541 kb on + strand, within BWI76_RS13100 at 2733.580 kb on + strand, within BWI76_RS13100 at 2733.580 kb on + strand, within BWI76_RS13100
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 2,731,077 - BWI76_RS13085 0.79 -0.5 2,731,806 + -0.3 2,731,992 - BWI76_RS13090 0.22 -1.3 2,732,023 - BWI76_RS13090 0.25 -3.2 2,732,123 + BWI76_RS13090 0.36 +0.3 2,732,224 - BWI76_RS13090 0.47 -3.0 2,732,879 + BWI76_RS13095 0.51 +1.0 2,732,982 + BWI76_RS13095 0.87 -0.7 2,732,990 - -0.9 2,733,007 + -0.5 2,733,042 - +0.8 2,733,362 + BWI76_RS13100 0.44 -0.7 2,733,402 + BWI76_RS13100 0.50 +0.8 2,733,453 + BWI76_RS13100 0.56 -1.4 2,733,541 + BWI76_RS13100 0.68 -0.1 2,733,580 + BWI76_RS13100 0.73 +0.1 2,733,580 + BWI76_RS13100 0.73 +1.1
Or see this region's nucleotide sequence