Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11320 and BWI76_RS11325 are separated by 20 nucleotides BWI76_RS11325 and BWI76_RS11330 are separated by 236 nucleotides BWI76_RS11330 and BWI76_RS11335 are separated by 156 nucleotides BWI76_RS11335 and BWI76_RS11340 are separated by 5 nucleotides BWI76_RS11340 and BWI76_RS11345 are separated by 100 nucleotides
BWI76_RS11320: BWI76_RS11320 - NUDIX hydrolase, at 2,386,433 to 2,386,909
_RS11320
BWI76_RS11325: BWI76_RS11325 - 23S rRNA pseudouridine synthase E, at 2,386,930 to 2,387,580
_RS11325
BWI76_RS11330: BWI76_RS11330 - isocitrate dehydrogenase (NADP(+)), at 2,387,817 to 2,389,067
_RS11330
BWI76_RS11335: BWI76_RS11335 - tautomerase, at 2,389,224 to 2,389,610
_RS11335
BWI76_RS11340: BWI76_RS11340 - carboxymuconolactone decarboxylase, at 2,389,616 to 2,389,918
_RS11340
BWI76_RS11345: BWI76_RS11345 - LysR family transcriptional regulator, at 2,390,019 to 2,390,903
_RS11345
Position (kb)
2387
2388
2389
2390 Strain fitness (log2 ratio)
-2
-1
0
1 at 2387.180 kb on - strand, within BWI76_RS11325 at 2387.561 kb on + strand at 2387.608 kb on - strand at 2387.769 kb on + strand at 2389.183 kb on - strand at 2389.230 kb on - strand at 2389.232 kb on + strand at 2389.346 kb on + strand, within BWI76_RS11335 at 2389.581 kb on - strand at 2389.613 kb on + strand at 2389.748 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 2,387,180 - BWI76_RS11325 0.38 -0.3 2,387,561 + +0.9 2,387,608 - +0.9 2,387,769 + -2.0 2,389,183 - +1.0 2,389,230 - -0.7 2,389,232 + -0.3 2,389,346 + BWI76_RS11335 0.32 -2.3 2,389,581 - +0.7 2,389,613 + -0.8 2,389,748 - +0.0
Or see this region's nucleotide sequence