Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS04145

Experiment: 2'-Deoxyinosine 5 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS04140 and BWI76_RS04145 are separated by 94 nucleotidesBWI76_RS04145 and BWI76_RS04150 are separated by 58 nucleotides BWI76_RS04140: BWI76_RS04140 - phosphopentomutase, at 851,800 to 853,023 _RS04140 BWI76_RS04145: BWI76_RS04145 - purine-nucleoside phosphorylase, at 853,118 to 853,840 _RS04145 BWI76_RS04150: BWI76_RS04150 - lipoate--protein ligase A, at 853,899 to 854,915 _RS04150 Position (kb) 853 854Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2at 852.195 kb on + strand, within BWI76_RS04140at 852.378 kb on + strand, within BWI76_RS04140at 852.585 kb on + strand, within BWI76_RS04140at 852.856 kb on + strand, within BWI76_RS04140at 852.866 kb on + strand, within BWI76_RS04140at 852.948 kb on - strandat 853.171 kb on + strandat 853.670 kb on + strand, within BWI76_RS04145at 853.696 kb on - strand, within BWI76_RS04145at 853.751 kb on + strand, within BWI76_RS04145at 853.789 kb on - strandat 853.815 kb on + strandat 854.125 kb on - strand, within BWI76_RS04150at 854.125 kb on - strand, within BWI76_RS04150at 854.264 kb on - strand, within BWI76_RS04150at 854.274 kb on - strand, within BWI76_RS04150at 854.379 kb on + strand, within BWI76_RS04150at 854.386 kb on + strand, within BWI76_RS04150at 854.387 kb on - strand, within BWI76_RS04150at 854.505 kb on + strand, within BWI76_RS04150at 854.513 kb on - strand, within BWI76_RS04150at 854.670 kb on - strand, within BWI76_RS04150

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Per-strain Table

Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C)
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852,195 + BWI76_RS04140 0.32 +0.1
852,378 + BWI76_RS04140 0.47 +0.1
852,585 + BWI76_RS04140 0.64 -5.4
852,856 + BWI76_RS04140 0.86 -1.9
852,866 + BWI76_RS04140 0.87 -1.9
852,948 - -4.2
853,171 + -1.8
853,670 + BWI76_RS04145 0.76 -2.9
853,696 - BWI76_RS04145 0.80 -0.5
853,751 + BWI76_RS04145 0.88 -0.5
853,789 - -2.7
853,815 + -1.1
854,125 - BWI76_RS04150 0.22 -0.7
854,125 - BWI76_RS04150 0.22 +0.0
854,264 - BWI76_RS04150 0.36 +0.4
854,274 - BWI76_RS04150 0.37 -0.1
854,379 + BWI76_RS04150 0.47 +0.4
854,386 + BWI76_RS04150 0.48 +0.1
854,387 - BWI76_RS04150 0.48 +1.8
854,505 + BWI76_RS04150 0.60 +0.1
854,513 - BWI76_RS04150 0.60 +0.3
854,670 - BWI76_RS04150 0.76 +0.8

Or see this region's nucleotide sequence