Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS00505

Experiment: 2'-Deoxyinosine 5 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS00495 and BWI76_RS00500 overlap by 8 nucleotidesBWI76_RS00500 and BWI76_RS00505 are separated by 11 nucleotidesBWI76_RS00505 and BWI76_RS00510 are separated by 13 nucleotides BWI76_RS00495: BWI76_RS00495 - PTS mannose transporter subunit IID, at 101,771 to 102,613 _RS00495 BWI76_RS00500: BWI76_RS00500 - PTS sorbose IIC component, at 102,606 to 103,409 _RS00500 BWI76_RS00505: BWI76_RS00505 - PTS system, sorbose-specific IIB component, at 103,421 to 103,918 _RS00505 BWI76_RS00510: BWI76_RS00510 - glucuronyl hydrolase, at 103,932 to 105,110 _RS00510 Position (kb) 103 104Strain fitness (log2 ratio) -2 -1 0 1at 102.487 kb on - strand, within BWI76_RS00495at 102.505 kb on - strand, within BWI76_RS00495at 102.589 kb on + strandat 102.589 kb on + strandat 102.589 kb on + strandat 102.597 kb on - strandat 102.597 kb on - strandat 102.597 kb on - strandat 102.601 kb on + strandat 102.621 kb on - strandat 102.626 kb on + strandat 102.713 kb on - strand, within BWI76_RS00500at 102.816 kb on - strand, within BWI76_RS00500at 102.818 kb on - strand, within BWI76_RS00500at 102.827 kb on + strand, within BWI76_RS00500at 102.828 kb on - strand, within BWI76_RS00500at 102.851 kb on + strand, within BWI76_RS00500at 102.928 kb on + strand, within BWI76_RS00500at 102.936 kb on - strand, within BWI76_RS00500at 102.985 kb on + strand, within BWI76_RS00500at 103.017 kb on + strand, within BWI76_RS00500at 103.025 kb on - strand, within BWI76_RS00500at 103.058 kb on + strand, within BWI76_RS00500at 103.063 kb on + strand, within BWI76_RS00500at 103.063 kb on + strand, within BWI76_RS00500at 103.066 kb on - strand, within BWI76_RS00500at 103.071 kb on - strand, within BWI76_RS00500at 103.302 kb on - strand, within BWI76_RS00500at 103.309 kb on + strand, within BWI76_RS00500at 103.346 kb on + strandat 103.346 kb on + strandat 103.360 kb on + strandat 103.505 kb on + strand, within BWI76_RS00505at 103.720 kb on - strand, within BWI76_RS00505at 103.824 kb on - strand, within BWI76_RS00505at 103.845 kb on + strand, within BWI76_RS00505at 103.865 kb on - strand, within BWI76_RS00505at 103.933 kb on - strandat 103.944 kb on + strandat 103.963 kb on - strandat 103.968 kb on + strandat 104.111 kb on + strand, within BWI76_RS00510at 104.119 kb on - strand, within BWI76_RS00510at 104.119 kb on - strand, within BWI76_RS00510at 104.119 kb on - strand, within BWI76_RS00510at 104.223 kb on - strand, within BWI76_RS00510at 104.265 kb on + strand, within BWI76_RS00510at 104.341 kb on + strand, within BWI76_RS00510at 104.347 kb on - strand, within BWI76_RS00510at 104.349 kb on - strand, within BWI76_RS00510at 104.349 kb on - strand, within BWI76_RS00510at 104.460 kb on - strand, within BWI76_RS00510at 104.654 kb on - strand, within BWI76_RS00510at 104.727 kb on - strand, within BWI76_RS00510at 104.765 kb on - strand, within BWI76_RS00510at 104.772 kb on - strand, within BWI76_RS00510at 104.796 kb on - strand, within BWI76_RS00510at 104.829 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C)
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102,487 - BWI76_RS00495 0.85 +0.5
102,505 - BWI76_RS00495 0.87 +0.1
102,589 + -0.6
102,589 + -0.7
102,589 + -0.5
102,597 - -1.1
102,597 - -0.2
102,597 - +0.7
102,601 + -0.1
102,621 - +0.6
102,626 + -0.1
102,713 - BWI76_RS00500 0.13 +0.8
102,816 - BWI76_RS00500 0.26 -0.2
102,818 - BWI76_RS00500 0.26 +1.3
102,827 + BWI76_RS00500 0.27 +0.9
102,828 - BWI76_RS00500 0.28 +0.3
102,851 + BWI76_RS00500 0.30 -1.0
102,928 + BWI76_RS00500 0.40 -0.2
102,936 - BWI76_RS00500 0.41 -0.0
102,985 + BWI76_RS00500 0.47 -0.2
103,017 + BWI76_RS00500 0.51 -0.5
103,025 - BWI76_RS00500 0.52 -0.1
103,058 + BWI76_RS00500 0.56 -1.6
103,063 + BWI76_RS00500 0.57 +0.9
103,063 + BWI76_RS00500 0.57 +1.0
103,066 - BWI76_RS00500 0.57 -1.7
103,071 - BWI76_RS00500 0.58 -1.7
103,302 - BWI76_RS00500 0.87 +0.6
103,309 + BWI76_RS00500 0.87 +0.4
103,346 + +0.2
103,346 + +0.3
103,360 + +0.8
103,505 + BWI76_RS00505 0.17 -2.0
103,720 - BWI76_RS00505 0.60 -1.4
103,824 - BWI76_RS00505 0.81 -0.2
103,845 + BWI76_RS00505 0.85 -2.2
103,865 - BWI76_RS00505 0.89 -2.0
103,933 - -0.3
103,944 + +1.4
103,963 - -0.2
103,968 + -0.1
104,111 + BWI76_RS00510 0.15 +1.0
104,119 - BWI76_RS00510 0.16 -0.7
104,119 - BWI76_RS00510 0.16 -0.1
104,119 - BWI76_RS00510 0.16 -0.3
104,223 - BWI76_RS00510 0.25 -0.9
104,265 + BWI76_RS00510 0.28 +0.8
104,341 + BWI76_RS00510 0.35 +0.3
104,347 - BWI76_RS00510 0.35 -0.5
104,349 - BWI76_RS00510 0.35 +0.5
104,349 - BWI76_RS00510 0.35 +1.6
104,460 - BWI76_RS00510 0.45 +0.4
104,654 - BWI76_RS00510 0.61 -0.6
104,727 - BWI76_RS00510 0.67 +0.1
104,765 - BWI76_RS00510 0.71 -0.2
104,772 - BWI76_RS00510 0.71 -0.6
104,796 - BWI76_RS00510 0.73 -0.3
104,829 + +0.8

Or see this region's nucleotide sequence