Experiment: KB with Cotarnine Chloride 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2725 and Psyr_2726 overlap by 4 nucleotides Psyr_2726 and Psyr_2727 are separated by 167 nucleotides
Psyr_2725: Psyr_2725 - Thiolase, at 3,306,708 to 3,307,970
_2725
Psyr_2726: Psyr_2726 - trans-feruloyl-CoA synthase, at 3,307,967 to 3,309,868
_2726
Psyr_2727: Psyr_2727 - vanillin dehydrogenase, at 3,310,036 to 3,311,484
_2727
Position (kb)
3307
3308
3309
3310 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3307.175 kb on + strand, within Psyr_2725 at 3307.175 kb on + strand, within Psyr_2725 at 3307.175 kb on + strand, within Psyr_2725 at 3307.176 kb on - strand, within Psyr_2725 at 3307.298 kb on + strand, within Psyr_2725 at 3307.299 kb on - strand, within Psyr_2725 at 3307.299 kb on - strand, within Psyr_2725 at 3307.499 kb on - strand, within Psyr_2725 at 3307.565 kb on + strand, within Psyr_2725 at 3307.567 kb on + strand, within Psyr_2725 at 3307.568 kb on - strand, within Psyr_2725 at 3307.682 kb on + strand, within Psyr_2725 at 3307.682 kb on + strand, within Psyr_2725 at 3307.683 kb on - strand, within Psyr_2725 at 3307.683 kb on - strand, within Psyr_2725 at 3307.727 kb on - strand, within Psyr_2725 at 3307.753 kb on + strand, within Psyr_2725 at 3307.754 kb on - strand, within Psyr_2725 at 3307.792 kb on - strand, within Psyr_2725 at 3307.952 kb on - strand at 3308.013 kb on + strand at 3308.014 kb on - strand at 3308.216 kb on + strand, within Psyr_2726 at 3308.338 kb on - strand, within Psyr_2726 at 3308.338 kb on - strand, within Psyr_2726 at 3308.493 kb on + strand, within Psyr_2726 at 3308.493 kb on + strand, within Psyr_2726 at 3308.493 kb on + strand, within Psyr_2726 at 3308.494 kb on - strand, within Psyr_2726 at 3308.494 kb on - strand, within Psyr_2726 at 3308.812 kb on + strand, within Psyr_2726 at 3308.895 kb on + strand, within Psyr_2726 at 3308.973 kb on + strand, within Psyr_2726 at 3308.973 kb on + strand, within Psyr_2726 at 3308.974 kb on - strand, within Psyr_2726 at 3308.974 kb on - strand, within Psyr_2726 at 3308.974 kb on - strand, within Psyr_2726 at 3308.991 kb on + strand, within Psyr_2726 at 3308.991 kb on + strand, within Psyr_2726 at 3308.991 kb on + strand, within Psyr_2726 at 3309.368 kb on + strand, within Psyr_2726 at 3309.368 kb on + strand, within Psyr_2726 at 3309.369 kb on - strand, within Psyr_2726 at 3309.369 kb on - strand, within Psyr_2726 at 3309.369 kb on - strand, within Psyr_2726 at 3309.369 kb on - strand, within Psyr_2726 at 3309.471 kb on + strand, within Psyr_2726 at 3309.471 kb on + strand, within Psyr_2726 at 3309.472 kb on - strand, within Psyr_2726 at 3309.511 kb on + strand, within Psyr_2726 at 3309.811 kb on + strand at 3309.812 kb on - strand at 3309.874 kb on + strand at 3310.128 kb on - strand at 3310.655 kb on + strand, within Psyr_2727 at 3310.655 kb on + strand, within Psyr_2727 at 3310.656 kb on - strand, within Psyr_2727 at 3310.656 kb on - strand, within Psyr_2727 at 3310.656 kb on - strand, within Psyr_2727 at 3310.789 kb on + strand, within Psyr_2727 at 3310.790 kb on - strand, within Psyr_2727 at 3310.790 kb on - strand, within Psyr_2727
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Cotarnine Chloride 10 mM remove 3,307,175 + Psyr_2725 0.37 -0.5 3,307,175 + Psyr_2725 0.37 -0.4 3,307,175 + Psyr_2725 0.37 -0.1 3,307,176 - Psyr_2725 0.37 +0.7 3,307,298 + Psyr_2725 0.47 +1.4 3,307,299 - Psyr_2725 0.47 +0.6 3,307,299 - Psyr_2725 0.47 -0.4 3,307,499 - Psyr_2725 0.63 +2.3 3,307,565 + Psyr_2725 0.68 +0.6 3,307,567 + Psyr_2725 0.68 +0.9 3,307,568 - Psyr_2725 0.68 -0.5 3,307,682 + Psyr_2725 0.77 -0.8 3,307,682 + Psyr_2725 0.77 -0.8 3,307,683 - Psyr_2725 0.77 -0.4 3,307,683 - Psyr_2725 0.77 +0.1 3,307,727 - Psyr_2725 0.81 -0.3 3,307,753 + Psyr_2725 0.83 -0.4 3,307,754 - Psyr_2725 0.83 +0.7 3,307,792 - Psyr_2725 0.86 +0.3 3,307,952 - -2.6 3,308,013 + -0.8 3,308,014 - -0.5 3,308,216 + Psyr_2726 0.13 +1.0 3,308,338 - Psyr_2726 0.20 +0.3 3,308,338 - Psyr_2726 0.20 +0.3 3,308,493 + Psyr_2726 0.28 +0.0 3,308,493 + Psyr_2726 0.28 +0.8 3,308,493 + Psyr_2726 0.28 -0.7 3,308,494 - Psyr_2726 0.28 +1.3 3,308,494 - Psyr_2726 0.28 +1.2 3,308,812 + Psyr_2726 0.44 +1.0 3,308,895 + Psyr_2726 0.49 +0.0 3,308,973 + Psyr_2726 0.53 -0.1 3,308,973 + Psyr_2726 0.53 +0.9 3,308,974 - Psyr_2726 0.53 -0.8 3,308,974 - Psyr_2726 0.53 +0.7 3,308,974 - Psyr_2726 0.53 +0.3 3,308,991 + Psyr_2726 0.54 +0.8 3,308,991 + Psyr_2726 0.54 +0.9 3,308,991 + Psyr_2726 0.54 +0.8 3,309,368 + Psyr_2726 0.74 +0.2 3,309,368 + Psyr_2726 0.74 -0.1 3,309,369 - Psyr_2726 0.74 +1.2 3,309,369 - Psyr_2726 0.74 -1.3 3,309,369 - Psyr_2726 0.74 +0.5 3,309,369 - Psyr_2726 0.74 +0.1 3,309,471 + Psyr_2726 0.79 -0.6 3,309,471 + Psyr_2726 0.79 +0.7 3,309,472 - Psyr_2726 0.79 +0.7 3,309,511 + Psyr_2726 0.81 -0.3 3,309,811 + -2.3 3,309,812 - -0.1 3,309,874 + +0.5 3,310,128 - +0.7 3,310,655 + Psyr_2727 0.43 +0.1 3,310,655 + Psyr_2727 0.43 +0.4 3,310,656 - Psyr_2727 0.43 -1.0 3,310,656 - Psyr_2727 0.43 -0.6 3,310,656 - Psyr_2727 0.43 +2.3 3,310,789 + Psyr_2727 0.52 +0.7 3,310,790 - Psyr_2727 0.52 +0.0 3,310,790 - Psyr_2727 0.52 +0.1
Or see this region's nucleotide sequence