Experiment: Sucrose (C) and Glycine betaine (N); with MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS02125 and PFLU_RS02130 overlap by 17 nucleotides PFLU_RS02130 and PFLU_RS02135 overlap by 4 nucleotides PFLU_RS02135 and PFLU_RS02140 are separated by 149 nucleotides
PFLU_RS02125: PFLU_RS02125 - aromatic amino acid lyase, at 478,405 to 479,946
_RS02125
PFLU_RS02130: PFLU_RS02130 - glycosyl transferase, at 479,930 to 480,865
_RS02130
PFLU_RS02135: PFLU_RS02135 - glycosyltransferase family 2 protein, at 480,862 to 481,596
_RS02135
PFLU_RS02140: PFLU_RS02140 - AMP-binding protein, at 481,746 to 483,416
_RS02140
Position (kb) 480
481
482 Strain fitness (log2 ratio) -1
0
1 at 479.887 kb on + strand at 479.887 kb on + strand at 479.887 kb on + strand at 479.888 kb on - strand at 479.888 kb on - strand at 479.888 kb on - strand at 479.888 kb on - strand at 480.021 kb on + strand at 480.021 kb on + strand at 480.021 kb on + strand at 480.021 kb on + strand at 480.022 kb on - strand at 480.022 kb on - strand at 480.687 kb on + strand, within PFLU_RS02130 at 480.687 kb on + strand, within PFLU_RS02130 at 480.733 kb on - strand, within PFLU_RS02130 at 480.747 kb on + strand, within PFLU_RS02130 at 480.748 kb on - strand, within PFLU_RS02130 at 480.817 kb on - strand at 481.085 kb on + strand, within PFLU_RS02135 at 481.085 kb on + strand, within PFLU_RS02135 at 481.085 kb on + strand, within PFLU_RS02135 at 481.085 kb on + strand, within PFLU_RS02135 at 481.086 kb on - strand, within PFLU_RS02135 at 481.086 kb on - strand, within PFLU_RS02135 at 481.086 kb on - strand, within PFLU_RS02135 at 481.086 kb on - strand, within PFLU_RS02135 at 481.199 kb on + strand, within PFLU_RS02135 at 481.208 kb on + strand, within PFLU_RS02135 at 481.208 kb on + strand, within PFLU_RS02135 at 481.209 kb on - strand, within PFLU_RS02135 at 481.209 kb on - strand, within PFLU_RS02135 at 481.209 kb on - strand, within PFLU_RS02135 at 481.565 kb on + strand at 481.565 kb on + strand at 481.565 kb on + strand at 481.565 kb on + strand at 481.566 kb on - strand at 481.566 kb on - strand at 481.566 kb on - strand at 481.566 kb on - strand at 481.566 kb on - strand at 481.566 kb on - strand at 481.566 kb on - strand at 481.566 kb on - strand at 481.566 kb on - strand at 481.566 kb on - strand at 481.591 kb on - strand at 481.591 kb on - strand at 481.678 kb on + strand at 481.678 kb on + strand at 481.678 kb on + strand at 481.678 kb on + strand at 481.679 kb on - strand at 481.679 kb on - strand at 481.679 kb on - strand at 481.747 kb on + strand at 481.747 kb on + strand at 481.747 kb on + strand at 481.747 kb on + strand at 481.747 kb on + strand at 481.747 kb on + strand at 481.747 kb on + strand at 481.747 kb on + strand at 481.747 kb on + strand at 481.747 kb on + strand at 481.747 kb on + strand at 481.747 kb on + strand at 481.747 kb on + strand at 481.747 kb on + strand at 481.747 kb on + strand at 481.748 kb on - strand at 481.748 kb on - strand at 481.748 kb on - strand at 481.748 kb on - strand at 481.748 kb on - strand at 481.748 kb on - strand at 481.748 kb on - strand at 481.748 kb on - strand at 481.748 kb on - strand at 481.748 kb on - strand at 481.748 kb on - strand at 481.748 kb on - strand at 481.748 kb on - strand at 481.816 kb on + strand at 481.816 kb on + strand at 481.816 kb on + strand at 481.816 kb on + strand at 481.816 kb on + strand at 481.816 kb on + strand at 481.816 kb on + strand at 481.816 kb on + strand at 481.817 kb on - strand at 481.817 kb on - strand at 481.817 kb on - strand at 482.014 kb on + strand, within PFLU_RS02140 at 482.015 kb on - strand, within PFLU_RS02140 at 482.015 kb on - strand, within PFLU_RS02140 at 482.015 kb on - strand, within PFLU_RS02140 at 482.513 kb on - strand, within PFLU_RS02140
Per-strain Table
Position Strand Gene LocusTag Fraction Sucrose (C) and Glycine betaine (N); with MOPS remove 479,887 + +0.4 479,887 + -0.7 479,887 + +1.3 479,888 - +0.0 479,888 - +0.9 479,888 - +0.3 479,888 - -0.6 480,021 + +0.3 480,021 + +0.7 480,021 + -0.6 480,021 + +1.1 480,022 - +0.2 480,022 - +0.3 480,687 + PFLU_RS02130 0.81 -1.4 480,687 + PFLU_RS02130 0.81 +0.2 480,733 - PFLU_RS02130 0.86 +0.6 480,747 + PFLU_RS02130 0.87 -0.3 480,748 - PFLU_RS02130 0.87 +1.0 480,817 - +0.4 481,085 + PFLU_RS02135 0.30 +1.2 481,085 + PFLU_RS02135 0.30 +1.0 481,085 + PFLU_RS02135 0.30 -0.2 481,085 + PFLU_RS02135 0.30 -0.3 481,086 - PFLU_RS02135 0.30 -0.4 481,086 - PFLU_RS02135 0.30 +0.6 481,086 - PFLU_RS02135 0.30 +0.1 481,086 - PFLU_RS02135 0.30 +0.4 481,199 + PFLU_RS02135 0.46 +0.5 481,208 + PFLU_RS02135 0.47 +1.7 481,208 + PFLU_RS02135 0.47 -0.2 481,209 - PFLU_RS02135 0.47 -0.7 481,209 - PFLU_RS02135 0.47 +0.1 481,209 - PFLU_RS02135 0.47 +0.4 481,565 + -1.1 481,565 + -1.1 481,565 + +0.9 481,565 + -0.7 481,566 - +0.9 481,566 - +0.4 481,566 - -0.2 481,566 - +0.3 481,566 - -0.9 481,566 - +0.3 481,566 - -0.1 481,566 - +0.2 481,566 - +1.3 481,566 - +0.8 481,591 - -0.0 481,591 - +0.7 481,678 + -0.5 481,678 + -0.2 481,678 + +0.1 481,678 + +0.8 481,679 - -0.0 481,679 - +0.2 481,679 - +1.0 481,747 + +0.4 481,747 + +1.0 481,747 + +0.2 481,747 + +0.4 481,747 + -0.9 481,747 + -0.2 481,747 + +0.8 481,747 + -0.1 481,747 + +0.6 481,747 + +0.5 481,747 + +0.2 481,747 + +0.3 481,747 + +0.3 481,747 + -0.2 481,747 + -0.3 481,748 - +0.1 481,748 - -0.8 481,748 - -0.7 481,748 - +1.0 481,748 - +0.8 481,748 - -0.0 481,748 - +0.2 481,748 - +0.3 481,748 - -0.5 481,748 - +0.8 481,748 - -0.5 481,748 - -0.4 481,748 - -1.4 481,816 + -0.5 481,816 + +0.2 481,816 + +0.2 481,816 + -0.1 481,816 + -0.0 481,816 + -0.5 481,816 + +0.2 481,816 + -0.1 481,817 - -0.2 481,817 - +0.3 481,817 - -1.0 482,014 + PFLU_RS02140 0.16 -0.7 482,015 - PFLU_RS02140 0.16 +0.6 482,015 - PFLU_RS02140 0.16 +1.1 482,015 - PFLU_RS02140 0.16 +0.7 482,513 - PFLU_RS02140 0.46 +0.8
Or see this region's nucleotide sequence