Experiment: Sucrose (C) and Glycine betaine (N); with MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS01910 and PFLU_RS01915 overlap by 1 nucleotides PFLU_RS01915 and PFLU_RS01920 are separated by 144 nucleotides PFLU_RS01920 and PFLU_RS01925 are separated by 105 nucleotides PFLU_RS01925 and PFLU_RS01930 are separated by 12 nucleotides
PFLU_RS01910: PFLU_RS01910 - sterol-binding protein, at 429,536 to 430,159
_RS01910
PFLU_RS01915: PFLU_RS01915 - bifunctional demethylmenaquinone methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol methylase UbiE, at 430,159 to 430,929
_RS01915
PFLU_RS01920: PFLU_RS01920 - hypothetical protein, at 431,074 to 431,349
_RS01920
PFLU_RS01925: PFLU_RS01925 - phasin family protein, at 431,455 to 431,877
_RS01925
PFLU_RS01930: PFLU_RS01930 - phasin family protein, at 431,890 to 432,837
_RS01930
Position (kb) 431
432 Strain fitness (log2 ratio) -2
-1
0
1
2 at 430.930 kb on - strand at 430.930 kb on - strand at 430.963 kb on + strand at 430.963 kb on + strand at 430.963 kb on + strand at 430.963 kb on + strand at 430.963 kb on + strand at 430.964 kb on - strand at 430.964 kb on - strand at 430.964 kb on - strand at 430.964 kb on - strand at 431.002 kb on + strand at 431.002 kb on + strand at 431.002 kb on + strand at 431.002 kb on + strand at 431.002 kb on + strand at 431.002 kb on + strand at 431.002 kb on + strand at 431.002 kb on + strand at 431.002 kb on + strand at 431.002 kb on + strand at 431.002 kb on + strand at 431.003 kb on - strand at 431.003 kb on - strand at 431.003 kb on - strand at 431.003 kb on - strand at 431.003 kb on - strand at 431.003 kb on - strand at 431.003 kb on - strand at 431.003 kb on - strand at 431.003 kb on - strand at 431.042 kb on + strand at 431.043 kb on - strand at 431.054 kb on + strand at 431.057 kb on - strand at 431.057 kb on - strand at 431.057 kb on - strand at 431.082 kb on + strand at 431.082 kb on + strand at 431.082 kb on + strand at 431.082 kb on + strand at 431.082 kb on + strand at 431.082 kb on + strand at 431.083 kb on - strand at 431.083 kb on - strand at 431.083 kb on - strand at 431.083 kb on - strand at 431.083 kb on - strand at 431.083 kb on - strand at 431.083 kb on - strand at 431.085 kb on - strand at 431.085 kb on - strand at 431.112 kb on + strand, within PFLU_RS01920 at 431.167 kb on + strand, within PFLU_RS01920 at 431.167 kb on + strand, within PFLU_RS01920 at 431.167 kb on + strand, within PFLU_RS01920 at 431.168 kb on - strand, within PFLU_RS01920 at 431.196 kb on + strand, within PFLU_RS01920 at 431.196 kb on + strand, within PFLU_RS01920 at 431.224 kb on - strand, within PFLU_RS01920 at 431.234 kb on + strand, within PFLU_RS01920 at 431.234 kb on + strand, within PFLU_RS01920 at 431.235 kb on - strand, within PFLU_RS01920 at 431.235 kb on - strand, within PFLU_RS01920 at 431.235 kb on - strand, within PFLU_RS01920 at 431.237 kb on + strand, within PFLU_RS01920 at 431.237 kb on + strand, within PFLU_RS01920 at 431.237 kb on + strand, within PFLU_RS01920 at 431.362 kb on + strand at 431.363 kb on - strand at 431.363 kb on - strand at 431.389 kb on + strand at 431.389 kb on + strand at 431.389 kb on + strand at 431.390 kb on - strand at 431.390 kb on - strand at 431.390 kb on - strand at 431.390 kb on - strand at 431.390 kb on - strand at 431.436 kb on - strand at 431.499 kb on + strand, within PFLU_RS01925 at 431.499 kb on + strand, within PFLU_RS01925 at 431.514 kb on + strand, within PFLU_RS01925 at 431.514 kb on + strand, within PFLU_RS01925 at 431.515 kb on - strand, within PFLU_RS01925 at 431.515 kb on - strand, within PFLU_RS01925 at 431.515 kb on - strand, within PFLU_RS01925 at 431.557 kb on + strand, within PFLU_RS01925 at 431.557 kb on + strand, within PFLU_RS01925 at 431.558 kb on - strand, within PFLU_RS01925 at 431.604 kb on + strand, within PFLU_RS01925 at 431.628 kb on + strand, within PFLU_RS01925 at 431.687 kb on + strand, within PFLU_RS01925 at 431.687 kb on + strand, within PFLU_RS01925 at 431.687 kb on + strand, within PFLU_RS01925 at 431.687 kb on + strand, within PFLU_RS01925 at 431.688 kb on - strand, within PFLU_RS01925 at 431.688 kb on - strand, within PFLU_RS01925 at 431.694 kb on + strand, within PFLU_RS01925 at 431.694 kb on + strand, within PFLU_RS01925 at 431.694 kb on + strand, within PFLU_RS01925 at 431.694 kb on + strand, within PFLU_RS01925 at 431.694 kb on + strand, within PFLU_RS01925 at 431.694 kb on + strand, within PFLU_RS01925 at 431.695 kb on - strand, within PFLU_RS01925 at 431.695 kb on - strand, within PFLU_RS01925 at 431.695 kb on - strand, within PFLU_RS01925 at 431.695 kb on - strand, within PFLU_RS01925 at 431.695 kb on - strand, within PFLU_RS01925 at 431.695 kb on - strand, within PFLU_RS01925 at 431.695 kb on - strand, within PFLU_RS01925 at 431.695 kb on - strand, within PFLU_RS01925 at 431.720 kb on + strand, within PFLU_RS01925 at 431.721 kb on - strand, within PFLU_RS01925 at 431.721 kb on - strand, within PFLU_RS01925 at 431.721 kb on - strand, within PFLU_RS01925 at 431.736 kb on + strand, within PFLU_RS01925 at 431.736 kb on + strand, within PFLU_RS01925 at 431.757 kb on + strand, within PFLU_RS01925 at 431.758 kb on - strand, within PFLU_RS01925 at 431.799 kb on + strand, within PFLU_RS01925 at 431.826 kb on + strand, within PFLU_RS01925 at 431.827 kb on - strand, within PFLU_RS01925 at 431.875 kb on + strand at 431.875 kb on + strand at 431.875 kb on + strand at 431.875 kb on + strand at 431.876 kb on - strand at 431.876 kb on - strand at 431.876 kb on - strand at 431.907 kb on + strand at 431.908 kb on - strand at 431.958 kb on + strand at 431.959 kb on - strand at 431.959 kb on - strand at 431.978 kb on + strand at 431.979 kb on - strand at 431.979 kb on - strand at 431.979 kb on - strand at 431.987 kb on - strand, within PFLU_RS01930 at 431.987 kb on - strand, within PFLU_RS01930 at 431.987 kb on - strand, within PFLU_RS01930 at 431.987 kb on - strand, within PFLU_RS01930 at 432.016 kb on + strand, within PFLU_RS01930 at 432.016 kb on + strand, within PFLU_RS01930 at 432.016 kb on + strand, within PFLU_RS01930 at 432.016 kb on + strand, within PFLU_RS01930 at 432.016 kb on + strand, within PFLU_RS01930 at 432.016 kb on + strand, within PFLU_RS01930 at 432.016 kb on + strand, within PFLU_RS01930 at 432.016 kb on + strand, within PFLU_RS01930 at 432.016 kb on + strand, within PFLU_RS01930 at 432.017 kb on - strand, within PFLU_RS01930 at 432.078 kb on + strand, within PFLU_RS01930 at 432.078 kb on + strand, within PFLU_RS01930 at 432.079 kb on - strand, within PFLU_RS01930 at 432.079 kb on - strand, within PFLU_RS01930 at 432.079 kb on - strand, within PFLU_RS01930
Per-strain Table
Position Strand Gene LocusTag Fraction Sucrose (C) and Glycine betaine (N); with MOPS remove 430,930 - -1.0 430,930 - +0.3 430,963 + +1.1 430,963 + +0.2 430,963 + +1.3 430,963 + -0.2 430,963 + +0.4 430,964 - -0.2 430,964 - -1.5 430,964 - +0.8 430,964 - -0.0 431,002 + +0.3 431,002 + +0.9 431,002 + -0.3 431,002 + -0.2 431,002 + +0.1 431,002 + +1.0 431,002 + +0.6 431,002 + +0.2 431,002 + -0.5 431,002 + +0.7 431,002 + -0.0 431,003 - -0.4 431,003 - +0.4 431,003 - +0.7 431,003 - -0.9 431,003 - -0.7 431,003 - +0.1 431,003 - +0.4 431,003 - +0.1 431,003 - -0.3 431,042 + +0.3 431,043 - -0.0 431,054 + +1.3 431,057 - +1.9 431,057 - +0.8 431,057 - +1.0 431,082 + -1.1 431,082 + +0.7 431,082 + +0.7 431,082 + +0.8 431,082 + +0.4 431,082 + -0.1 431,083 - -1.9 431,083 - +0.3 431,083 - +0.3 431,083 - -2.7 431,083 - +0.3 431,083 - -2.6 431,083 - -0.3 431,085 - +0.6 431,085 - -0.3 431,112 + PFLU_RS01920 0.14 +0.9 431,167 + PFLU_RS01920 0.34 -0.1 431,167 + PFLU_RS01920 0.34 +1.1 431,167 + PFLU_RS01920 0.34 +0.3 431,168 - PFLU_RS01920 0.34 +0.8 431,196 + PFLU_RS01920 0.44 +0.4 431,196 + PFLU_RS01920 0.44 -0.0 431,224 - PFLU_RS01920 0.54 +0.7 431,234 + PFLU_RS01920 0.58 +0.2 431,234 + PFLU_RS01920 0.58 +0.4 431,235 - PFLU_RS01920 0.58 +0.4 431,235 - PFLU_RS01920 0.58 +0.5 431,235 - PFLU_RS01920 0.58 -0.3 431,237 + PFLU_RS01920 0.59 +0.3 431,237 + PFLU_RS01920 0.59 +0.3 431,237 + PFLU_RS01920 0.59 +0.7 431,362 + +0.8 431,363 - +1.2 431,363 - +1.2 431,389 + -0.0 431,389 + +0.3 431,389 + +0.5 431,390 - -0.8 431,390 - +0.4 431,390 - +1.3 431,390 - +0.7 431,390 - -0.2 431,436 - +0.1 431,499 + PFLU_RS01925 0.10 +1.3 431,499 + PFLU_RS01925 0.10 +0.4 431,514 + PFLU_RS01925 0.14 +0.8 431,514 + PFLU_RS01925 0.14 -0.1 431,515 - PFLU_RS01925 0.14 +0.6 431,515 - PFLU_RS01925 0.14 -0.9 431,515 - PFLU_RS01925 0.14 -0.8 431,557 + PFLU_RS01925 0.24 +1.0 431,557 + PFLU_RS01925 0.24 -2.0 431,558 - PFLU_RS01925 0.24 -0.8 431,604 + PFLU_RS01925 0.35 +1.0 431,628 + PFLU_RS01925 0.41 -0.4 431,687 + PFLU_RS01925 0.55 +0.3 431,687 + PFLU_RS01925 0.55 +1.7 431,687 + PFLU_RS01925 0.55 -0.8 431,687 + PFLU_RS01925 0.55 -0.3 431,688 - PFLU_RS01925 0.55 -1.8 431,688 - PFLU_RS01925 0.55 -0.8 431,694 + PFLU_RS01925 0.57 +0.2 431,694 + PFLU_RS01925 0.57 +0.3 431,694 + PFLU_RS01925 0.57 -0.1 431,694 + PFLU_RS01925 0.57 -0.3 431,694 + PFLU_RS01925 0.57 +0.4 431,694 + PFLU_RS01925 0.57 +0.8 431,695 - PFLU_RS01925 0.57 +0.3 431,695 - PFLU_RS01925 0.57 -0.0 431,695 - PFLU_RS01925 0.57 -0.1 431,695 - PFLU_RS01925 0.57 +0.4 431,695 - PFLU_RS01925 0.57 -0.3 431,695 - PFLU_RS01925 0.57 -0.6 431,695 - PFLU_RS01925 0.57 -0.6 431,695 - PFLU_RS01925 0.57 -0.5 431,720 + PFLU_RS01925 0.63 +1.6 431,721 - PFLU_RS01925 0.63 -0.7 431,721 - PFLU_RS01925 0.63 +0.1 431,721 - PFLU_RS01925 0.63 +0.6 431,736 + PFLU_RS01925 0.66 +0.5 431,736 + PFLU_RS01925 0.66 +0.2 431,757 + PFLU_RS01925 0.71 -0.2 431,758 - PFLU_RS01925 0.72 +0.4 431,799 + PFLU_RS01925 0.81 -0.8 431,826 + PFLU_RS01925 0.88 +0.7 431,827 - PFLU_RS01925 0.88 +0.8 431,875 + -0.8 431,875 + +0.6 431,875 + +0.4 431,875 + +0.8 431,876 - +0.7 431,876 - +0.1 431,876 - -0.2 431,907 + -0.1 431,908 - +0.5 431,958 + +0.5 431,959 - -2.4 431,959 - +0.6 431,978 + +0.7 431,979 - +0.6 431,979 - -0.1 431,979 - +0.4 431,987 - PFLU_RS01930 0.10 -1.2 431,987 - PFLU_RS01930 0.10 -1.4 431,987 - PFLU_RS01930 0.10 +0.2 431,987 - PFLU_RS01930 0.10 -2.0 432,016 + PFLU_RS01930 0.13 -2.5 432,016 + PFLU_RS01930 0.13 -1.0 432,016 + PFLU_RS01930 0.13 +0.6 432,016 + PFLU_RS01930 0.13 -0.4 432,016 + PFLU_RS01930 0.13 -0.3 432,016 + PFLU_RS01930 0.13 +0.4 432,016 + PFLU_RS01930 0.13 -0.3 432,016 + PFLU_RS01930 0.13 -0.3 432,016 + PFLU_RS01930 0.13 +0.1 432,017 - PFLU_RS01930 0.13 -0.6 432,078 + PFLU_RS01930 0.20 +0.1 432,078 + PFLU_RS01930 0.20 +0.2 432,079 - PFLU_RS01930 0.20 +0.2 432,079 - PFLU_RS01930 0.20 +0.9 432,079 - PFLU_RS01930 0.20 +1.8
Or see this region's nucleotide sequence