Experiment: 2'-Deoxyinosine 10 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS24630 and BWI76_RS24635 are separated by 74 nucleotides BWI76_RS24635 and BWI76_RS24640 overlap by 4 nucleotides BWI76_RS24640 and BWI76_RS24645 are separated by 11 nucleotides
BWI76_RS24630: BWI76_RS24630 - putrescine aminotransferase, at 5,133,326 to 5,134,732
_RS24630
BWI76_RS24635: BWI76_RS24635 - autoinducer-2 (AI-2) modifying protein LsrG, at 5,134,807 to 5,135,103
_RS24635
BWI76_RS24640: BWI76_RS24640 - aldolase, at 5,135,100 to 5,135,987
_RS24640
BWI76_RS24645: BWI76_RS24645 - autoinducer 2 ABC transporter substrate-binding protein, at 5,135,999 to 5,137,000
_RS24645
Position (kb) 5134
5135
5136 Strain fitness (log2 ratio) -2
-1
0
1 at 5133.914 kb on + strand, within BWI76_RS24630 at 5134.078 kb on - strand, within BWI76_RS24630 at 5134.153 kb on + strand, within BWI76_RS24630 at 5134.369 kb on - strand, within BWI76_RS24630 at 5134.415 kb on - strand, within BWI76_RS24630 at 5134.482 kb on + strand, within BWI76_RS24630 at 5134.561 kb on - strand, within BWI76_RS24630 at 5134.786 kb on + strand at 5134.804 kb on + strand at 5134.804 kb on + strand at 5134.812 kb on - strand at 5134.812 kb on - strand at 5134.875 kb on + strand, within BWI76_RS24635 at 5134.909 kb on - strand, within BWI76_RS24635 at 5135.021 kb on + strand, within BWI76_RS24635 at 5135.041 kb on - strand, within BWI76_RS24635 at 5135.136 kb on - strand at 5135.285 kb on - strand, within BWI76_RS24640 at 5135.330 kb on - strand, within BWI76_RS24640 at 5135.490 kb on - strand, within BWI76_RS24640 at 5135.496 kb on - strand, within BWI76_RS24640 at 5135.557 kb on + strand, within BWI76_RS24640 at 5135.565 kb on - strand, within BWI76_RS24640 at 5135.722 kb on - strand, within BWI76_RS24640 at 5135.754 kb on - strand, within BWI76_RS24640 at 5135.938 kb on - strand at 5135.948 kb on - strand at 5136.097 kb on - strand at 5136.101 kb on + strand, within BWI76_RS24645
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 10 mM (C) remove 5,133,914 + BWI76_RS24630 0.42 -0.1 5,134,078 - BWI76_RS24630 0.53 +0.6 5,134,153 + BWI76_RS24630 0.59 +0.9 5,134,369 - BWI76_RS24630 0.74 +0.7 5,134,415 - BWI76_RS24630 0.77 -0.1 5,134,482 + BWI76_RS24630 0.82 -1.7 5,134,561 - BWI76_RS24630 0.88 +0.0 5,134,786 + +0.2 5,134,804 + -0.3 5,134,804 + +0.1 5,134,812 - -0.4 5,134,812 - +0.4 5,134,875 + BWI76_RS24635 0.23 +1.0 5,134,909 - BWI76_RS24635 0.34 -2.3 5,135,021 + BWI76_RS24635 0.72 -1.1 5,135,041 - BWI76_RS24635 0.79 -1.0 5,135,136 - -0.6 5,135,285 - BWI76_RS24640 0.21 +0.2 5,135,330 - BWI76_RS24640 0.26 -0.2 5,135,490 - BWI76_RS24640 0.44 +0.7 5,135,496 - BWI76_RS24640 0.45 -0.8 5,135,557 + BWI76_RS24640 0.51 -0.6 5,135,565 - BWI76_RS24640 0.52 -0.1 5,135,722 - BWI76_RS24640 0.70 +0.6 5,135,754 - BWI76_RS24640 0.74 -0.4 5,135,938 - -0.1 5,135,948 - +0.9 5,136,097 - -0.5 5,136,101 + BWI76_RS24645 0.10 +0.4
Or see this region's nucleotide sequence