Experiment: 2'-Deoxyinosine 10 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05155 and BWI76_RS05160 are separated by 92 nucleotides BWI76_RS05160 and BWI76_RS05165 are separated by 180 nucleotides BWI76_RS05165 and BWI76_RS05170 overlap by 8 nucleotides BWI76_RS05170 and BWI76_RS05175 are separated by 84 nucleotides
BWI76_RS05155: BWI76_RS05155 - iron-sulfur cluster insertion protein ErpA, at 1,085,131 to 1,085,475
_RS05155
BWI76_RS05160: BWI76_RS05160 - hypothetical protein, at 1,085,568 to 1,086,191
_RS05160
BWI76_RS05165: BWI76_RS05165 - cobalamin-binding protein, at 1,086,372 to 1,087,172
_RS05165
BWI76_RS05170: BWI76_RS05170 - 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase, at 1,087,165 to 1,087,863
_RS05170
BWI76_RS05175: BWI76_RS05175 - dGTPase, at 1,087,948 to 1,089,462
_RS05175
Position (kb) 1086
1087
1088 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1085.778 kb on + strand, within BWI76_RS05160 at 1085.855 kb on + strand, within BWI76_RS05160 at 1085.884 kb on + strand, within BWI76_RS05160 at 1086.174 kb on - strand at 1086.197 kb on + strand at 1086.204 kb on + strand at 1086.306 kb on + strand at 1086.314 kb on - strand at 1086.399 kb on + strand at 1086.425 kb on - strand at 1086.445 kb on - strand at 1086.540 kb on + strand, within BWI76_RS05165 at 1086.566 kb on + strand, within BWI76_RS05165 at 1086.574 kb on - strand, within BWI76_RS05165 at 1086.666 kb on + strand, within BWI76_RS05165 at 1087.021 kb on + strand, within BWI76_RS05165 at 1087.094 kb on - strand at 1087.133 kb on + strand at 1088.154 kb on + strand, within BWI76_RS05175 at 1088.156 kb on + strand, within BWI76_RS05175 at 1088.172 kb on - strand, within BWI76_RS05175
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 10 mM (C) remove 1,085,778 + BWI76_RS05160 0.34 +0.1 1,085,855 + BWI76_RS05160 0.46 +0.7 1,085,884 + BWI76_RS05160 0.51 -0.1 1,086,174 - -2.0 1,086,197 + -1.0 1,086,204 + -0.0 1,086,306 + -0.3 1,086,314 - +1.1 1,086,399 + -0.8 1,086,425 - +0.2 1,086,445 - -0.0 1,086,540 + BWI76_RS05165 0.21 -0.8 1,086,566 + BWI76_RS05165 0.24 -0.7 1,086,574 - BWI76_RS05165 0.25 -0.4 1,086,666 + BWI76_RS05165 0.37 -2.5 1,087,021 + BWI76_RS05165 0.81 -2.7 1,087,094 - +2.0 1,087,133 + +0.9 1,088,154 + BWI76_RS05175 0.14 -0.1 1,088,156 + BWI76_RS05175 0.14 +0.8 1,088,172 - BWI76_RS05175 0.15 -2.7
Or see this region's nucleotide sequence