Experiment: fumarate electron acceptor
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fliA and flhG overlap by 8 nucleotides flhG and flhF are separated by 34 nucleotides flhF and flhA are separated by 11 nucleotides
SO3210: fliA - RNA polymerase sigma-27 factor (NCBI ptt file), at 3,350,155 to 3,350,868
fliA
SO3211: flhG - flagellar biosynthetic protein FlhG (NCBI ptt file), at 3,350,861 to 3,351,712
flhG
SO3212: flhF - flagellar biosynthetic protein FlhF (NCBI ptt file), at 3,351,747 to 3,353,123
flhF
SO3213: flhA - flagellar biosynthesis protein FlhA (NCBI ptt file), at 3,353,135 to 3,355,234
flhA
Position (kb)
3351
3352
3353
3354 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3350.845 kb on + strand at 3350.853 kb on - strand at 3350.853 kb on - strand at 3350.853 kb on - strand at 3351.311 kb on - strand, within flhG at 3351.457 kb on - strand, within flhG at 3351.511 kb on - strand, within flhG at 3351.571 kb on - strand, within flhG at 3351.591 kb on + strand, within flhG at 3351.638 kb on + strand at 3351.646 kb on - strand at 3351.770 kb on - strand at 3351.770 kb on - strand at 3351.982 kb on - strand, within flhF at 3352.025 kb on - strand, within flhF at 3352.032 kb on + strand, within flhF at 3352.045 kb on - strand, within flhF at 3352.109 kb on + strand, within flhF at 3352.260 kb on + strand, within flhF at 3352.310 kb on - strand, within flhF at 3352.383 kb on + strand, within flhF at 3352.621 kb on - strand, within flhF at 3352.690 kb on + strand, within flhF at 3352.743 kb on - strand, within flhF at 3352.896 kb on + strand, within flhF at 3353.048 kb on + strand at 3353.056 kb on - strand at 3353.143 kb on - strand at 3353.234 kb on + strand at 3353.262 kb on - strand at 3353.506 kb on + strand, within flhA at 3353.689 kb on - strand, within flhA at 3353.788 kb on + strand, within flhA at 3353.795 kb on + strand, within flhA at 3353.906 kb on - strand, within flhA at 3353.986 kb on + strand, within flhA at 3354.057 kb on + strand, within flhA at 3354.057 kb on + strand, within flhA at 3354.065 kb on - strand, within flhA
Per-strain Table
Position Strand Gene LocusTag Fraction fumarate electron acceptor remove 3,350,845 + -1.8 3,350,853 - -1.6 3,350,853 - +0.6 3,350,853 - -0.3 3,351,311 - flhG SO3211 0.53 -2.0 3,351,457 - flhG SO3211 0.70 -2.6 3,351,511 - flhG SO3211 0.76 -1.6 3,351,571 - flhG SO3211 0.83 +0.5 3,351,591 + flhG SO3211 0.86 -1.0 3,351,638 + +1.8 3,351,646 - +2.1 3,351,770 - -0.3 3,351,770 - -2.2 3,351,982 - flhF SO3212 0.17 +2.7 3,352,025 - flhF SO3212 0.20 +1.7 3,352,032 + flhF SO3212 0.21 -0.8 3,352,045 - flhF SO3212 0.22 -1.7 3,352,109 + flhF SO3212 0.26 +0.2 3,352,260 + flhF SO3212 0.37 -0.5 3,352,310 - flhF SO3212 0.41 -0.6 3,352,383 + flhF SO3212 0.46 -0.2 3,352,621 - flhF SO3212 0.63 +0.8 3,352,690 + flhF SO3212 0.68 +0.2 3,352,743 - flhF SO3212 0.72 +4.4 3,352,896 + flhF SO3212 0.83 -1.3 3,353,048 + +0.8 3,353,056 - +1.1 3,353,143 - -0.3 3,353,234 + -2.2 3,353,262 - +3.2 3,353,506 + flhA SO3213 0.18 -1.6 3,353,689 - flhA SO3213 0.26 +0.9 3,353,788 + flhA SO3213 0.31 -2.8 3,353,795 + flhA SO3213 0.31 -1.5 3,353,906 - flhA SO3213 0.37 +1.0 3,353,986 + flhA SO3213 0.41 +2.0 3,354,057 + flhA SO3213 0.44 +3.0 3,354,057 + flhA SO3213 0.44 +1.4 3,354,065 - flhA SO3213 0.44 +1.5
Or see this region's nucleotide sequence