Experiment: fumarate electron acceptor
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pflB and pflA are separated by 63 nucleotides pflA and SO2914 are separated by 16 nucleotides SO2914 and ackA are separated by 44 nucleotides
SO2912: pflB - formate acetyltransferase (NCBI ptt file), at 3,042,162 to 3,044,444
pflB
SO2913: pflA - pyruvate formate-lyase 1 activating enzyme (NCBI ptt file), at 3,044,508 to 3,045,248
pflA
SO2914: SO2914 - conserved hypothetical protein (NCBI ptt file), at 3,045,265 to 3,045,804
SO2914
SO2915: ackA - acetate kinase (NCBI ptt file), at 3,045,849 to 3,047,048
ackA
Position (kb)
3044
3045
3046 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 3043.561 kb on - strand, within pflB at 3043.577 kb on - strand, within pflB at 3043.606 kb on + strand, within pflB at 3043.701 kb on + strand, within pflB at 3043.731 kb on - strand, within pflB at 3043.793 kb on + strand, within pflB at 3043.793 kb on + strand, within pflB at 3043.987 kb on + strand, within pflB at 3044.012 kb on + strand, within pflB at 3044.118 kb on - strand, within pflB at 3044.126 kb on + strand, within pflB at 3044.162 kb on - strand, within pflB at 3044.201 kb on - strand, within pflB at 3044.218 kb on - strand at 3044.223 kb on + strand at 3044.234 kb on - strand at 3044.392 kb on + strand at 3044.392 kb on + strand at 3044.400 kb on - strand at 3044.400 kb on - strand at 3044.525 kb on + strand at 3044.525 kb on + strand at 3044.525 kb on + strand at 3044.533 kb on - strand at 3044.533 kb on - strand at 3044.624 kb on - strand, within pflA at 3044.893 kb on - strand, within pflA at 3044.941 kb on - strand, within pflA at 3044.948 kb on + strand, within pflA at 3045.006 kb on - strand, within pflA at 3045.048 kb on + strand, within pflA at 3045.048 kb on + strand, within pflA at 3045.049 kb on - strand, within pflA at 3045.177 kb on + strand at 3045.185 kb on - strand at 3045.229 kb on + strand at 3045.394 kb on + strand at 3045.424 kb on + strand, within SO2914 at 3045.551 kb on - strand, within SO2914 at 3045.591 kb on + strand, within SO2914 at 3045.616 kb on - strand, within SO2914 at 3045.725 kb on - strand, within SO2914 at 3045.762 kb on - strand at 3045.775 kb on - strand at 3046.095 kb on + strand, within ackA at 3046.118 kb on - strand, within ackA at 3046.217 kb on - strand, within ackA at 3046.220 kb on - strand, within ackA at 3046.222 kb on - strand, within ackA at 3046.222 kb on - strand, within ackA
Per-strain Table
Position Strand Gene LocusTag Fraction fumarate electron acceptor remove 3,043,561 - pflB SO2912 0.61 -1.6 3,043,577 - pflB SO2912 0.62 -1.0 3,043,606 + pflB SO2912 0.63 -4.7 3,043,701 + pflB SO2912 0.67 -1.8 3,043,731 - pflB SO2912 0.69 -4.7 3,043,793 + pflB SO2912 0.71 -2.8 3,043,793 + pflB SO2912 0.71 -1.8 3,043,987 + pflB SO2912 0.80 -0.7 3,044,012 + pflB SO2912 0.81 -5.0 3,044,118 - pflB SO2912 0.86 -1.8 3,044,126 + pflB SO2912 0.86 -0.1 3,044,162 - pflB SO2912 0.88 -2.8 3,044,201 - pflB SO2912 0.89 -1.6 3,044,218 - -3.0 3,044,223 + -3.3 3,044,234 - +0.6 3,044,392 + -2.3 3,044,392 + +2.0 3,044,400 - -0.4 3,044,400 - -2.6 3,044,525 + -0.0 3,044,525 + -3.1 3,044,525 + -1.4 3,044,533 - -2.7 3,044,533 - -1.4 3,044,624 - pflA SO2913 0.16 -3.0 3,044,893 - pflA SO2913 0.52 -0.4 3,044,941 - pflA SO2913 0.58 -0.4 3,044,948 + pflA SO2913 0.59 -0.4 3,045,006 - pflA SO2913 0.67 -2.7 3,045,048 + pflA SO2913 0.73 -1.6 3,045,048 + pflA SO2913 0.73 -2.0 3,045,049 - pflA SO2913 0.73 -1.3 3,045,177 + -1.4 3,045,185 - -2.0 3,045,229 + -1.0 3,045,394 + -0.0 3,045,424 + SO2914 0.29 +0.3 3,045,551 - SO2914 0.53 -1.2 3,045,591 + SO2914 0.60 -2.0 3,045,616 - SO2914 0.65 -3.0 3,045,725 - SO2914 0.85 -1.3 3,045,762 - -1.9 3,045,775 - -1.4 3,046,095 + ackA SO2915 0.20 +0.6 3,046,118 - ackA SO2915 0.22 -2.3 3,046,217 - ackA SO2915 0.31 -2.8 3,046,220 - ackA SO2915 0.31 -2.8 3,046,222 - ackA SO2915 0.31 -0.4 3,046,222 - ackA SO2915 0.31 -1.6
Or see this region's nucleotide sequence