Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS27455

Experiment: 2-Deoxyadenosine 4 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS27445 and BWI76_RS27450 are separated by 95 nucleotidesBWI76_RS27450 and BWI76_RS27455 are separated by 0 nucleotidesBWI76_RS27455 and BWI76_RS27460 are separated by 112 nucleotidesBWI76_RS27460 and BWI76_RS27465 are separated by 108 nucleotides BWI76_RS27445: BWI76_RS27445 - fructose-bisphosphatase class II, at 5,694,899 to 5,695,909 _RS27445 BWI76_RS27450: BWI76_RS27450 - ferredoxin--NADP(+) reductase, at 5,696,005 to 5,696,751 _RS27450 BWI76_RS27455: BWI76_RS27455 - DUF805 domain-containing protein, at 5,696,752 to 5,697,180 _RS27455 BWI76_RS27460: BWI76_RS27460 - hypothetical protein, at 5,697,293 to 5,697,892 _RS27460 BWI76_RS27465: BWI76_RS27465 - triose-phosphate isomerase, at 5,698,001 to 5,698,768 _RS27465 Position (kb) 5696 5697 5698Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 5695.771 kb on + strand, within BWI76_RS27445at 5695.880 kb on + strandat 5696.061 kb on + strandat 5696.392 kb on + strand, within BWI76_RS27450at 5696.400 kb on - strand, within BWI76_RS27450at 5696.429 kb on - strand, within BWI76_RS27450at 5696.497 kb on - strand, within BWI76_RS27450at 5696.627 kb on + strand, within BWI76_RS27450at 5696.637 kb on - strand, within BWI76_RS27450at 5696.864 kb on + strand, within BWI76_RS27455at 5696.942 kb on + strand, within BWI76_RS27455at 5697.030 kb on + strand, within BWI76_RS27455at 5697.168 kb on + strandat 5697.168 kb on + strandat 5697.176 kb on - strandat 5697.176 kb on - strandat 5697.181 kb on + strandat 5697.360 kb on - strand, within BWI76_RS27460at 5697.457 kb on + strand, within BWI76_RS27460at 5697.518 kb on + strand, within BWI76_RS27460at 5697.518 kb on + strand, within BWI76_RS27460at 5697.659 kb on + strand, within BWI76_RS27460at 5697.673 kb on - strand, within BWI76_RS27460at 5697.864 kb on + strandat 5697.870 kb on - strandat 5697.921 kb on + strandat 5697.921 kb on + strandat 5697.929 kb on + strandat 5697.941 kb on + strandat 5697.943 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C)
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5,695,771 + BWI76_RS27445 0.86 -1.0
5,695,880 + +0.7
5,696,061 + +0.5
5,696,392 + BWI76_RS27450 0.52 +0.3
5,696,400 - BWI76_RS27450 0.53 +3.9
5,696,429 - BWI76_RS27450 0.57 +0.7
5,696,497 - BWI76_RS27450 0.66 -1.4
5,696,627 + BWI76_RS27450 0.83 -0.7
5,696,637 - BWI76_RS27450 0.85 +1.1
5,696,864 + BWI76_RS27455 0.26 -2.4
5,696,942 + BWI76_RS27455 0.44 +0.7
5,697,030 + BWI76_RS27455 0.65 -0.9
5,697,168 + -2.4
5,697,168 + +0.4
5,697,176 - +0.4
5,697,176 - +1.3
5,697,181 + +1.3
5,697,360 - BWI76_RS27460 0.11 +1.8
5,697,457 + BWI76_RS27460 0.27 +0.3
5,697,518 + BWI76_RS27460 0.38 -0.0
5,697,518 + BWI76_RS27460 0.38 +0.4
5,697,659 + BWI76_RS27460 0.61 +1.0
5,697,673 - BWI76_RS27460 0.63 -0.6
5,697,864 + -0.2
5,697,870 - -0.9
5,697,921 + +1.4
5,697,921 + +1.3
5,697,929 + +0.4
5,697,941 + +0.3
5,697,943 + -0.7

Or see this region's nucleotide sequence