Experiment: 2-Deoxyadenosine 4 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27420 and BWI76_RS27425 are separated by 54 nucleotides BWI76_RS27425 and BWI76_RS27430 are separated by 18 nucleotides BWI76_RS27430 and BWI76_RS27435 are separated by 4 nucleotides BWI76_RS27435 and BWI76_RS27440 are separated by 2 nucleotides
BWI76_RS27420: BWI76_RS27420 - guanylate kinase, at 5,688,975 to 5,689,598
_RS27420
BWI76_RS27425: BWI76_RS27425 - DNA-directed RNA polymerase subunit omega, at 5,689,653 to 5,689,928
_RS27425
BWI76_RS27430: BWI76_RS27430 - (p)ppGpp synthetase, at 5,689,947 to 5,692,067
_RS27430
BWI76_RS27435: BWI76_RS27435 - tRNA (guanosine(18)-2'-O)-methyltransferase TrmH, at 5,692,072 to 5,692,761
_RS27435
BWI76_RS27440: BWI76_RS27440 - DNA helicase RecG, at 5,692,764 to 5,694,845
_RS27440
Position (kb)
5689
5690
5691
5692
5693 Strain fitness (log2 ratio)
-2
-1
0
1 at 5689.709 kb on + strand, within BWI76_RS27425 at 5689.821 kb on + strand, within BWI76_RS27425 at 5689.821 kb on + strand, within BWI76_RS27425 at 5691.447 kb on - strand, within BWI76_RS27430 at 5691.721 kb on - strand, within BWI76_RS27430 at 5691.896 kb on + strand at 5692.004 kb on - strand at 5692.139 kb on + strand at 5692.287 kb on + strand, within BWI76_RS27435 at 5692.295 kb on - strand, within BWI76_RS27435 at 5692.295 kb on - strand, within BWI76_RS27435 at 5692.333 kb on + strand, within BWI76_RS27435 at 5692.333 kb on + strand, within BWI76_RS27435 at 5692.420 kb on - strand, within BWI76_RS27435 at 5692.471 kb on + strand, within BWI76_RS27435 at 5692.545 kb on + strand, within BWI76_RS27435 at 5692.553 kb on - strand, within BWI76_RS27435 at 5692.605 kb on + strand, within BWI76_RS27435 at 5692.678 kb on + strand, within BWI76_RS27435 at 5692.868 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C) remove 5,689,709 + BWI76_RS27425 0.20 +1.5 5,689,821 + BWI76_RS27425 0.61 -1.5 5,689,821 + BWI76_RS27425 0.61 -1.5 5,691,447 - BWI76_RS27430 0.71 +0.8 5,691,721 - BWI76_RS27430 0.84 +1.6 5,691,896 + -1.2 5,692,004 - -2.6 5,692,139 + -1.2 5,692,287 + BWI76_RS27435 0.31 -1.1 5,692,295 - BWI76_RS27435 0.32 -1.3 5,692,295 - BWI76_RS27435 0.32 -0.2 5,692,333 + BWI76_RS27435 0.38 -1.1 5,692,333 + BWI76_RS27435 0.38 -2.0 5,692,420 - BWI76_RS27435 0.50 -0.6 5,692,471 + BWI76_RS27435 0.58 +0.3 5,692,545 + BWI76_RS27435 0.69 -1.8 5,692,553 - BWI76_RS27435 0.70 -0.5 5,692,605 + BWI76_RS27435 0.77 -0.6 5,692,678 + BWI76_RS27435 0.88 +1.3 5,692,868 - -2.4
Or see this region's nucleotide sequence