Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS25935

Experiment: 2-Deoxyadenosine 4 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS25930 and BWI76_RS25935 overlap by 14 nucleotidesBWI76_RS25935 and BWI76_RS25940 overlap by 80 nucleotidesBWI76_RS25940 and BWI76_RS25945 are separated by 21 nucleotides BWI76_RS25930: BWI76_RS25930 - glutathione-regulated potassium-efflux system protein KefB, at 5,360,341 to 5,362,146 _RS25930 BWI76_RS25935: BWI76_RS25935 - glutathione-regulated potassium-efflux system ancillary protein KefG, at 5,362,133 to 5,362,693 _RS25935 BWI76_RS25940: BWI76_RS25940 - hypothetical protein, at 5,362,614 to 5,362,847 _RS25940 BWI76_RS25945: BWI76_RS25945 - ABC transporter ATP-binding protein, at 5,362,869 to 5,364,773 _RS25945 Position (kb) 5362 5363Strain fitness (log2 ratio) -2 -1 0 1 2at 5361.199 kb on + strand, within BWI76_RS25930at 5361.259 kb on + strand, within BWI76_RS25930at 5361.267 kb on - strand, within BWI76_RS25930at 5361.389 kb on + strand, within BWI76_RS25930at 5361.407 kb on - strand, within BWI76_RS25930at 5361.478 kb on + strand, within BWI76_RS25930at 5361.493 kb on + strand, within BWI76_RS25930at 5361.542 kb on - strand, within BWI76_RS25930at 5361.692 kb on + strand, within BWI76_RS25930at 5361.706 kb on - strand, within BWI76_RS25930at 5361.724 kb on + strand, within BWI76_RS25930at 5361.741 kb on - strand, within BWI76_RS25930at 5361.932 kb on - strand, within BWI76_RS25930at 5362.003 kb on + strandat 5362.211 kb on + strand, within BWI76_RS25935at 5362.438 kb on - strand, within BWI76_RS25935at 5362.480 kb on - strand, within BWI76_RS25935at 5362.480 kb on - strand, within BWI76_RS25935at 5362.927 kb on - strandat 5362.937 kb on + strandat 5363.187 kb on + strand, within BWI76_RS25945at 5363.678 kb on + strand, within BWI76_RS25945

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C)
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5,361,199 + BWI76_RS25930 0.48 +0.9
5,361,259 + BWI76_RS25930 0.51 +0.2
5,361,267 - BWI76_RS25930 0.51 -0.6
5,361,389 + BWI76_RS25930 0.58 -0.3
5,361,407 - BWI76_RS25930 0.59 -2.6
5,361,478 + BWI76_RS25930 0.63 +0.6
5,361,493 + BWI76_RS25930 0.64 -0.8
5,361,542 - BWI76_RS25930 0.67 +1.2
5,361,692 + BWI76_RS25930 0.75 +0.9
5,361,706 - BWI76_RS25930 0.76 +0.1
5,361,724 + BWI76_RS25930 0.77 -1.8
5,361,741 - BWI76_RS25930 0.78 -0.1
5,361,932 - BWI76_RS25930 0.88 -0.5
5,362,003 + +0.5
5,362,211 + BWI76_RS25935 0.14 +0.1
5,362,438 - BWI76_RS25935 0.54 +2.5
5,362,480 - BWI76_RS25935 0.62 -0.3
5,362,480 - BWI76_RS25935 0.62 +0.9
5,362,927 - +0.0
5,362,937 + +1.4
5,363,187 + BWI76_RS25945 0.17 -2.1
5,363,678 + BWI76_RS25945 0.42 -0.8

Or see this region's nucleotide sequence