Experiment: 2-Deoxyadenosine 4 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25275 and BWI76_RS25280 are separated by 45 nucleotides BWI76_RS25280 and BWI76_RS25285 overlap by 4 nucleotides BWI76_RS25285 and BWI76_RS25290 are separated by 53 nucleotides BWI76_RS25290 and BWI76_RS25295 overlap by 4 nucleotides
BWI76_RS25275: BWI76_RS25275 - PTS IIA-like nitrogen regulatory protein PtsN, at 5,253,656 to 5,254,144
_RS25275
BWI76_RS25280: BWI76_RS25280 - RNase adaptor protein RapZ, at 5,254,190 to 5,255,044
_RS25280
BWI76_RS25285: BWI76_RS25285 - HPr family phosphocarrier protein, at 5,255,041 to 5,255,313
_RS25285
BWI76_RS25290: BWI76_RS25290 - monofunctional biosynthetic peptidoglycan transglycosylase, at 5,255,367 to 5,256,092
_RS25290
BWI76_RS25295: BWI76_RS25295 - glutamine amidotransferase, at 5,256,089 to 5,256,742
_RS25295
Position (kb) 5255
5256 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 5254.184 kb on + strand at 5254.201 kb on - strand at 5254.555 kb on + strand, within BWI76_RS25280 at 5255.053 kb on - strand at 5255.069 kb on + strand, within BWI76_RS25285 at 5255.356 kb on - strand at 5255.396 kb on - strand at 5255.680 kb on - strand, within BWI76_RS25290 at 5255.899 kb on - strand, within BWI76_RS25290 at 5255.989 kb on + strand, within BWI76_RS25290 at 5255.989 kb on + strand, within BWI76_RS25290 at 5255.994 kb on + strand, within BWI76_RS25290 at 5255.997 kb on - strand, within BWI76_RS25290 at 5256.013 kb on + strand, within BWI76_RS25290 at 5256.021 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C) remove 5,254,184 + -2.2 5,254,201 - +1.4 5,254,555 + BWI76_RS25280 0.43 -0.8 5,255,053 - +0.4 5,255,069 + BWI76_RS25285 0.10 -1.4 5,255,356 - -2.5 5,255,396 - -0.6 5,255,680 - BWI76_RS25290 0.43 +0.7 5,255,899 - BWI76_RS25290 0.73 +0.6 5,255,989 + BWI76_RS25290 0.86 -0.1 5,255,989 + BWI76_RS25290 0.86 +3.2 5,255,994 + BWI76_RS25290 0.86 -1.6 5,255,997 - BWI76_RS25290 0.87 +0.7 5,256,013 + BWI76_RS25290 0.89 -0.6 5,256,021 - -0.8
Or see this region's nucleotide sequence