Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS25060

Experiment: 2-Deoxyadenosine 4 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS25055 and BWI76_RS25060 are separated by 173 nucleotidesBWI76_RS25060 and BWI76_RS25065 are separated by 107 nucleotides BWI76_RS25055: BWI76_RS25055 - DEAD/DEAH family ATP-dependent RNA helicase, at 5,215,319 to 5,217,280 _RS25055 BWI76_RS25060: BWI76_RS25060 - lipoprotein NlpI, at 5,217,454 to 5,218,338 _RS25060 BWI76_RS25065: BWI76_RS25065 - polyribonucleotide nucleotidyltransferase, at 5,218,446 to 5,220,584 _RS25065 Position (kb) 5217 5218 5219Strain fitness (log2 ratio) -1 0 1 2 3at 5217.685 kb on - strand, within BWI76_RS25060at 5217.919 kb on - strand, within BWI76_RS25060at 5218.150 kb on - strand, within BWI76_RS25060at 5218.177 kb on - strand, within BWI76_RS25060at 5218.197 kb on - strand, within BWI76_RS25060at 5218.294 kb on + strandat 5219.314 kb on + strand, within BWI76_RS25065

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C)
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5,217,685 - BWI76_RS25060 0.26 +1.9
5,217,919 - BWI76_RS25060 0.53 +2.1
5,218,150 - BWI76_RS25060 0.79 +1.6
5,218,177 - BWI76_RS25060 0.82 +1.7
5,218,197 - BWI76_RS25060 0.84 +2.8
5,218,294 + +2.8
5,219,314 + BWI76_RS25065 0.41 +2.8

Or see this region's nucleotide sequence