Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS23970

Experiment: 2-Deoxyadenosine 4 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS23960 and BWI76_RS23965 are separated by 90 nucleotidesBWI76_RS23965 and BWI76_RS23970 are separated by 150 nucleotidesBWI76_RS23970 and BWI76_RS23975 are separated by 188 nucleotides BWI76_RS23960: BWI76_RS23960 - oxidative stress defense protein, at 5,001,381 to 5,002,148 _RS23960 BWI76_RS23965: BWI76_RS23965 - arginine exporter protein, at 5,002,239 to 5,002,874 _RS23965 BWI76_RS23970: BWI76_RS23970 - mechanosensitive ion channel protein MscS, at 5,003,025 to 5,003,882 _RS23970 BWI76_RS23975: BWI76_RS23975 - class II fructose-bisphosphate aldolase, at 5,004,071 to 5,005,150 _RS23975 Position (kb) 5003 5004Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 5002.184 kb on - strandat 5002.252 kb on + strandat 5002.355 kb on + strand, within BWI76_RS23965at 5002.435 kb on - strand, within BWI76_RS23965at 5003.009 kb on + strandat 5003.220 kb on - strand, within BWI76_RS23970at 5003.415 kb on + strand, within BWI76_RS23970at 5003.415 kb on + strand, within BWI76_RS23970at 5003.538 kb on - strand, within BWI76_RS23970at 5003.800 kb on + strandat 5003.813 kb on - strandat 5003.834 kb on + strandat 5003.936 kb on - strandat 5003.944 kb on + strandat 5003.944 kb on + strandat 5003.944 kb on + strandat 5003.944 kb on + strandat 5003.944 kb on + strandat 5003.944 kb on + strandat 5003.952 kb on - strandat 5003.952 kb on - strandat 5003.952 kb on - strandat 5003.955 kb on + strandat 5003.957 kb on + strandat 5003.963 kb on - strandat 5003.963 kb on - strandat 5003.987 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C)
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5,002,184 - +0.2
5,002,252 + -1.2
5,002,355 + BWI76_RS23965 0.18 -0.0
5,002,435 - BWI76_RS23965 0.31 +0.6
5,003,009 + +0.4
5,003,220 - BWI76_RS23970 0.23 -2.1
5,003,415 + BWI76_RS23970 0.45 -3.9
5,003,415 + BWI76_RS23970 0.45 -1.2
5,003,538 - BWI76_RS23970 0.60 +0.1
5,003,800 + -0.7
5,003,813 - +0.5
5,003,834 + -0.8
5,003,936 - +0.5
5,003,944 + -2.7
5,003,944 + -0.2
5,003,944 + -1.1
5,003,944 + -0.5
5,003,944 + +1.4
5,003,944 + +0.4
5,003,952 - -2.2
5,003,952 - -1.4
5,003,952 - +0.2
5,003,955 + -0.2
5,003,957 + -0.2
5,003,963 - +0.7
5,003,963 - -0.6
5,003,987 + +0.7

Or see this region's nucleotide sequence