Experiment: 2-Deoxyadenosine 4 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS23960 and BWI76_RS23965 are separated by 90 nucleotides BWI76_RS23965 and BWI76_RS23970 are separated by 150 nucleotides BWI76_RS23970 and BWI76_RS23975 are separated by 188 nucleotides
BWI76_RS23960: BWI76_RS23960 - oxidative stress defense protein, at 5,001,381 to 5,002,148
_RS23960
BWI76_RS23965: BWI76_RS23965 - arginine exporter protein, at 5,002,239 to 5,002,874
_RS23965
BWI76_RS23970: BWI76_RS23970 - mechanosensitive ion channel protein MscS, at 5,003,025 to 5,003,882
_RS23970
BWI76_RS23975: BWI76_RS23975 - class II fructose-bisphosphate aldolase, at 5,004,071 to 5,005,150
_RS23975
Position (kb)
5003
5004 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 5002.184 kb on - strand at 5002.252 kb on + strand at 5002.355 kb on + strand, within BWI76_RS23965 at 5002.435 kb on - strand, within BWI76_RS23965 at 5003.009 kb on + strand at 5003.220 kb on - strand, within BWI76_RS23970 at 5003.415 kb on + strand, within BWI76_RS23970 at 5003.415 kb on + strand, within BWI76_RS23970 at 5003.538 kb on - strand, within BWI76_RS23970 at 5003.800 kb on + strand at 5003.813 kb on - strand at 5003.834 kb on + strand at 5003.936 kb on - strand at 5003.944 kb on + strand at 5003.944 kb on + strand at 5003.944 kb on + strand at 5003.944 kb on + strand at 5003.944 kb on + strand at 5003.944 kb on + strand at 5003.952 kb on - strand at 5003.952 kb on - strand at 5003.952 kb on - strand at 5003.955 kb on + strand at 5003.957 kb on + strand at 5003.963 kb on - strand at 5003.963 kb on - strand at 5003.987 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C) remove 5,002,184 - +0.2 5,002,252 + -1.2 5,002,355 + BWI76_RS23965 0.18 -0.0 5,002,435 - BWI76_RS23965 0.31 +0.6 5,003,009 + +0.4 5,003,220 - BWI76_RS23970 0.23 -2.1 5,003,415 + BWI76_RS23970 0.45 -3.9 5,003,415 + BWI76_RS23970 0.45 -1.2 5,003,538 - BWI76_RS23970 0.60 +0.1 5,003,800 + -0.7 5,003,813 - +0.5 5,003,834 + -0.8 5,003,936 - +0.5 5,003,944 + -2.7 5,003,944 + -0.2 5,003,944 + -1.1 5,003,944 + -0.5 5,003,944 + +1.4 5,003,944 + +0.4 5,003,952 - -2.2 5,003,952 - -1.4 5,003,952 - +0.2 5,003,955 + -0.2 5,003,957 + -0.2 5,003,963 - +0.7 5,003,963 - -0.6 5,003,987 + +0.7
Or see this region's nucleotide sequence