Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS21165

Experiment: 2-Deoxyadenosine 4 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS21160 and BWI76_RS21165 are separated by 177 nucleotidesBWI76_RS21165 and BWI76_RS21170 are separated by 233 nucleotides BWI76_RS21160: BWI76_RS21160 - IscS subfamily cysteine desulfurase, at 4,430,056 to 4,431,270 _RS21160 BWI76_RS21165: BWI76_RS21165 - Fe-S cluster assembly transcriptional regulator IscR, at 4,431,448 to 4,431,939 _RS21165 BWI76_RS21170: BWI76_RS21170 - tRNA (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ, at 4,432,173 to 4,432,904 _RS21170 Position (kb) 4431 4432Strain fitness (log2 ratio) -1 0 1at 4431.572 kb on - strand, within BWI76_RS21165at 4432.156 kb on + strandat 4432.639 kb on + strand, within BWI76_RS21170at 4432.647 kb on - strand, within BWI76_RS21170at 4432.809 kb on - strand, within BWI76_RS21170at 4432.820 kb on - strand, within BWI76_RS21170at 4432.851 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C)
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4,431,572 - BWI76_RS21165 0.25 +1.0
4,432,156 + -1.3
4,432,639 + BWI76_RS21170 0.64 -0.5
4,432,647 - BWI76_RS21170 0.65 -0.7
4,432,809 - BWI76_RS21170 0.87 -0.0
4,432,820 - BWI76_RS21170 0.88 +0.1
4,432,851 - -1.3

Or see this region's nucleotide sequence