Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS21140

Experiment: 2-Deoxyadenosine 4 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS21125 and BWI76_RS21130 are separated by 107 nucleotidesBWI76_RS21130 and BWI76_RS21135 are separated by 1 nucleotidesBWI76_RS21135 and BWI76_RS21140 are separated by 1 nucleotidesBWI76_RS21140 and BWI76_RS21145 are separated by 15 nucleotidesBWI76_RS21145 and BWI76_RS21150 are separated by 73 nucleotidesBWI76_RS21150 and BWI76_RS21155 are separated by 19 nucleotides BWI76_RS21125: BWI76_RS21125 - aminopeptidase PepB, at 4,424,912 to 4,426,198 _RS21125 BWI76_RS21130: BWI76_RS21130 - Fe-S assembly protein IscX, at 4,426,306 to 4,426,506 _RS21130 BWI76_RS21135: BWI76_RS21135 - ferredoxin, 2Fe-2S type, ISC system, at 4,426,508 to 4,426,843 _RS21135 BWI76_RS21140: BWI76_RS21140 - molecular chaperone HscA, at 4,426,845 to 4,428,695 _RS21140 BWI76_RS21145: BWI76_RS21145 - co-chaperone HscB, at 4,428,711 to 4,429,226 _RS21145 BWI76_RS21150: BWI76_RS21150 - iron-sulfur cluster assembly accessory protein, at 4,429,300 to 4,429,623 _RS21150 BWI76_RS21155: BWI76_RS21155 - iron-sulfur cluster scaffold-like protein, at 4,429,643 to 4,430,029 _RS21155 Position (kb) 4426 4427 4428 4429Strain fitness (log2 ratio) -3 -2 -1 0 1at 4425.899 kb on - strand, within BWI76_RS21125at 4425.952 kb on - strand, within BWI76_RS21125at 4426.228 kb on + strandat 4426.228 kb on + strandat 4426.228 kb on + strandat 4426.236 kb on - strandat 4426.236 kb on - strandat 4426.405 kb on - strand, within BWI76_RS21130at 4426.454 kb on + strand, within BWI76_RS21130at 4426.459 kb on + strandat 4426.490 kb on + strandat 4427.115 kb on - strand, within BWI76_RS21140at 4427.205 kb on - strand, within BWI76_RS21140at 4427.207 kb on + strand, within BWI76_RS21140at 4427.612 kb on + strand, within BWI76_RS21140at 4428.222 kb on + strand, within BWI76_RS21140at 4428.516 kb on - strandat 4428.758 kb on - strandat 4428.935 kb on + strand, within BWI76_RS21145at 4429.252 kb on + strandat 4429.284 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C)
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4,425,899 - BWI76_RS21125 0.77 +0.3
4,425,952 - BWI76_RS21125 0.81 +1.0
4,426,228 + +1.1
4,426,228 + -0.2
4,426,228 + -0.4
4,426,236 - +0.5
4,426,236 - -0.5
4,426,405 - BWI76_RS21130 0.49 -2.4
4,426,454 + BWI76_RS21130 0.74 -0.5
4,426,459 + +1.0
4,426,490 + +0.8
4,427,115 - BWI76_RS21140 0.15 -3.6
4,427,205 - BWI76_RS21140 0.19 -3.1
4,427,207 + BWI76_RS21140 0.20 -1.5
4,427,612 + BWI76_RS21140 0.41 -1.8
4,428,222 + BWI76_RS21140 0.74 -0.1
4,428,516 - -1.3
4,428,758 - +0.7
4,428,935 + BWI76_RS21145 0.43 -2.7
4,429,252 + +0.5
4,429,284 + -2.1

Or see this region's nucleotide sequence