Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS20900

Experiment: 2-Deoxyadenosine 4 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS20890 and BWI76_RS20895 are separated by 120 nucleotidesBWI76_RS20895 and BWI76_RS20900 are separated by 108 nucleotidesBWI76_RS20900 and BWI76_RS20905 are separated by 16 nucleotides BWI76_RS20890: BWI76_RS20890 - neutral zinc metallopeptidase, at 4,368,605 to 4,369,474 _RS20890 BWI76_RS20895: BWI76_RS20895 - phosphoribosylaminoimidazolesuccinocarboxamide synthase, at 4,369,595 to 4,370,308 _RS20895 BWI76_RS20900: BWI76_RS20900 - outer membrane protein assembly factor BamC, at 4,370,417 to 4,371,451 _RS20900 BWI76_RS20905: BWI76_RS20905 - 4-hydroxy-tetrahydrodipicolinate synthase, at 4,371,468 to 4,372,346 _RS20905 Position (kb) 4370 4371 4372Strain fitness (log2 ratio) -2 -1 0 1at 4369.646 kb on - strandat 4369.705 kb on - strand, within BWI76_RS20895at 4369.745 kb on - strand, within BWI76_RS20895at 4369.755 kb on + strand, within BWI76_RS20895at 4369.755 kb on + strand, within BWI76_RS20895at 4369.755 kb on + strand, within BWI76_RS20895at 4370.032 kb on - strand, within BWI76_RS20895at 4370.095 kb on + strand, within BWI76_RS20895at 4370.460 kb on + strandat 4370.759 kb on - strand, within BWI76_RS20900at 4370.981 kb on + strand, within BWI76_RS20900at 4371.449 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C)
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4,369,646 - +1.1
4,369,705 - BWI76_RS20895 0.15 +1.1
4,369,745 - BWI76_RS20895 0.21 -1.7
4,369,755 + BWI76_RS20895 0.22 -0.3
4,369,755 + BWI76_RS20895 0.22 -2.1
4,369,755 + BWI76_RS20895 0.22 +1.2
4,370,032 - BWI76_RS20895 0.61 -0.1
4,370,095 + BWI76_RS20895 0.70 -0.1
4,370,460 + -0.7
4,370,759 - BWI76_RS20900 0.33 -0.8
4,370,981 + BWI76_RS20900 0.54 -0.9
4,371,449 - -0.5

Or see this region's nucleotide sequence