Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS20890

Experiment: 2-Deoxyadenosine 4 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS20885 and BWI76_RS20890 are separated by 12 nucleotidesBWI76_RS20890 and BWI76_RS20895 are separated by 120 nucleotidesBWI76_RS20895 and BWI76_RS20900 are separated by 108 nucleotides BWI76_RS20885: BWI76_RS20885 - tRNA(Met) cytidine acetyltransferase TmcA, at 4,366,592 to 4,368,592 _RS20885 BWI76_RS20890: BWI76_RS20890 - neutral zinc metallopeptidase, at 4,368,605 to 4,369,474 _RS20890 BWI76_RS20895: BWI76_RS20895 - phosphoribosylaminoimidazolesuccinocarboxamide synthase, at 4,369,595 to 4,370,308 _RS20895 BWI76_RS20900: BWI76_RS20900 - outer membrane protein assembly factor BamC, at 4,370,417 to 4,371,451 _RS20900 Position (kb) 4368 4369 4370Strain fitness (log2 ratio) -2 -1 0 1 2at 4367.901 kb on + strand, within BWI76_RS20885at 4367.909 kb on - strand, within BWI76_RS20885at 4368.287 kb on + strand, within BWI76_RS20885at 4368.300 kb on - strand, within BWI76_RS20885at 4368.430 kb on + strandat 4368.438 kb on - strandat 4368.450 kb on - strandat 4368.470 kb on + strandat 4368.487 kb on - strandat 4368.632 kb on - strandat 4368.632 kb on - strandat 4368.806 kb on + strand, within BWI76_RS20890at 4368.836 kb on - strand, within BWI76_RS20890at 4368.978 kb on + strand, within BWI76_RS20890at 4368.986 kb on - strand, within BWI76_RS20890at 4368.986 kb on - strand, within BWI76_RS20890at 4369.069 kb on - strand, within BWI76_RS20890at 4369.071 kb on - strand, within BWI76_RS20890at 4369.646 kb on - strandat 4369.705 kb on - strand, within BWI76_RS20895at 4369.745 kb on - strand, within BWI76_RS20895at 4369.755 kb on + strand, within BWI76_RS20895at 4369.755 kb on + strand, within BWI76_RS20895at 4369.755 kb on + strand, within BWI76_RS20895at 4370.032 kb on - strand, within BWI76_RS20895at 4370.095 kb on + strand, within BWI76_RS20895at 4370.460 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C)
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4,367,901 + BWI76_RS20885 0.65 +0.6
4,367,909 - BWI76_RS20885 0.66 -0.1
4,368,287 + BWI76_RS20885 0.85 -0.5
4,368,300 - BWI76_RS20885 0.85 +0.2
4,368,430 + +0.7
4,368,438 - -0.4
4,368,450 - -0.7
4,368,470 + +0.1
4,368,487 - +0.1
4,368,632 - +0.0
4,368,632 - -1.6
4,368,806 + BWI76_RS20890 0.23 +1.1
4,368,836 - BWI76_RS20890 0.27 +2.4
4,368,978 + BWI76_RS20890 0.43 +1.9
4,368,986 - BWI76_RS20890 0.44 +0.4
4,368,986 - BWI76_RS20890 0.44 +0.0
4,369,069 - BWI76_RS20890 0.53 -0.5
4,369,071 - BWI76_RS20890 0.54 -1.3
4,369,646 - +1.1
4,369,705 - BWI76_RS20895 0.15 +1.1
4,369,745 - BWI76_RS20895 0.21 -1.7
4,369,755 + BWI76_RS20895 0.22 -0.3
4,369,755 + BWI76_RS20895 0.22 -2.1
4,369,755 + BWI76_RS20895 0.22 +1.2
4,370,032 - BWI76_RS20895 0.61 -0.1
4,370,095 + BWI76_RS20895 0.70 -0.1
4,370,460 + -0.7

Or see this region's nucleotide sequence