Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS20880

Experiment: 2-Deoxyadenosine 4 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS20870 and BWI76_RS20875 are separated by 27 nucleotidesBWI76_RS20875 and BWI76_RS20880 are separated by 232 nucleotidesBWI76_RS20880 and BWI76_RS20885 are separated by 77 nucleotides BWI76_RS20870: BWI76_RS20870 - succinyl-diaminopimelate desuccinylase, at 4,364,225 to 4,365,352 _RS20870 BWI76_RS20875: BWI76_RS20875 - membrane protein, at 4,365,380 to 4,365,583 _RS20875 BWI76_RS20880: BWI76_RS20880 - esterase, at 4,365,816 to 4,366,514 _RS20880 BWI76_RS20885: BWI76_RS20885 - tRNA(Met) cytidine acetyltransferase TmcA, at 4,366,592 to 4,368,592 _RS20885 Position (kb) 4365 4366 4367Strain fitness (log2 ratio) -2 -1 0 1at 4365.337 kb on + strandat 4365.407 kb on + strand, within BWI76_RS20875at 4366.189 kb on - strand, within BWI76_RS20880at 4366.394 kb on + strand, within BWI76_RS20880at 4366.824 kb on - strandat 4366.831 kb on - strand, within BWI76_RS20885at 4367.071 kb on + strand, within BWI76_RS20885at 4367.079 kb on - strand, within BWI76_RS20885

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C)
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4,365,337 + -1.0
4,365,407 + BWI76_RS20875 0.13 -1.9
4,366,189 - BWI76_RS20880 0.53 -2.2
4,366,394 + BWI76_RS20880 0.83 -0.8
4,366,824 - +1.7
4,366,831 - BWI76_RS20885 0.12 -0.3
4,367,071 + BWI76_RS20885 0.24 -1.5
4,367,079 - BWI76_RS20885 0.24 -0.5

Or see this region's nucleotide sequence