Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS20670

Experiment: 2-Deoxyadenosine 4 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS20660 and BWI76_RS20665 overlap by 4 nucleotidesBWI76_RS20665 and BWI76_RS20670 are separated by 82 nucleotidesBWI76_RS20670 and BWI76_RS20675 are separated by 14 nucleotidesBWI76_RS20675 and BWI76_RS20680 are separated by 45 nucleotides BWI76_RS20660: BWI76_RS20660 - hypothetical protein, at 4,323,185 to 4,323,424 _RS20660 BWI76_RS20665: BWI76_RS20665 - bifunctional pyridoxal kinase/hydroxymethylpyrimidine kinase, at 4,323,421 to 4,324,287 _RS20665 BWI76_RS20670: BWI76_RS20670 - transcriptional regulator PtsJ, at 4,324,370 to 4,325,668 _RS20670 BWI76_RS20675: BWI76_RS20675 - GMP synthase, at 4,325,683 to 4,326,390 _RS20675 BWI76_RS20680: BWI76_RS20680 - hypothetical protein, at 4,326,436 to 4,326,840 _RS20680 Position (kb) 4324 4325 4326Strain fitness (log2 ratio) -1 0 1at 4323.795 kb on + strand, within BWI76_RS20665at 4323.959 kb on + strand, within BWI76_RS20665at 4323.967 kb on - strand, within BWI76_RS20665at 4324.019 kb on + strand, within BWI76_RS20665at 4324.114 kb on - strand, within BWI76_RS20665at 4324.152 kb on + strand, within BWI76_RS20665at 4324.189 kb on + strand, within BWI76_RS20665at 4324.220 kb on + strandat 4324.515 kb on + strand, within BWI76_RS20670at 4324.533 kb on + strand, within BWI76_RS20670at 4325.054 kb on - strand, within BWI76_RS20670at 4325.140 kb on + strand, within BWI76_RS20670at 4325.549 kb on + strandat 4325.701 kb on - strandat 4325.726 kb on + strandat 4325.731 kb on + strandat 4325.779 kb on - strand, within BWI76_RS20675at 4325.864 kb on + strand, within BWI76_RS20675at 4325.904 kb on + strand, within BWI76_RS20675at 4326.249 kb on + strand, within BWI76_RS20675at 4326.249 kb on + strandat 4326.258 kb on - strand, within BWI76_RS20675at 4326.315 kb on + strand, within BWI76_RS20675

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C)
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4,323,795 + BWI76_RS20665 0.43 +0.8
4,323,959 + BWI76_RS20665 0.62 +0.6
4,323,967 - BWI76_RS20665 0.63 +0.1
4,324,019 + BWI76_RS20665 0.69 +0.4
4,324,114 - BWI76_RS20665 0.80 -0.6
4,324,152 + BWI76_RS20665 0.84 +0.9
4,324,189 + BWI76_RS20665 0.89 +0.8
4,324,220 + +1.5
4,324,515 + BWI76_RS20670 0.11 +0.9
4,324,533 + BWI76_RS20670 0.13 +0.2
4,325,054 - BWI76_RS20670 0.53 +0.9
4,325,140 + BWI76_RS20670 0.59 +0.5
4,325,549 + -0.3
4,325,701 - +1.1
4,325,726 + +0.7
4,325,731 + +0.5
4,325,779 - BWI76_RS20675 0.14 +0.2
4,325,864 + BWI76_RS20675 0.26 -0.7
4,325,904 + BWI76_RS20675 0.31 +0.1
4,326,249 + BWI76_RS20675 0.80 +0.8
4,326,249 + +0.5
4,326,258 - BWI76_RS20675 0.81 +0.1
4,326,315 + BWI76_RS20675 0.89 +0.9

Or see this region's nucleotide sequence