Experiment: 2-Deoxyadenosine 4 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20435 and BWI76_RS20440 are separated by 37 nucleotides BWI76_RS20440 and BWI76_RS20445 are separated by 167 nucleotides BWI76_RS20445 and BWI76_RS20450 are separated by 20 nucleotides
BWI76_RS20435: BWI76_RS20435 - chorismate synthase, at 4,277,326 to 4,278,411
_RS20435
BWI76_RS20440: BWI76_RS20440 - 50S ribosomal protein L3 N(5)-glutamine methyltransferase, at 4,278,449 to 4,279,381
_RS20440
BWI76_RS20445: BWI76_RS20445 - endonuclease SmrB, at 4,279,549 to 4,280,100
_RS20445
BWI76_RS20450: BWI76_RS20450 - phosphohistidine phosphatase, at 4,280,121 to 4,280,606
_RS20450
Position (kb) 4278
4279
4280 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4277.528 kb on + strand, within BWI76_RS20435 at 4278.248 kb on - strand, within BWI76_RS20435 at 4278.348 kb on - strand at 4278.588 kb on - strand, within BWI76_RS20440 at 4278.639 kb on + strand, within BWI76_RS20440 at 4278.867 kb on + strand, within BWI76_RS20440 at 4278.867 kb on + strand, within BWI76_RS20440 at 4279.395 kb on - strand at 4279.396 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C) remove 4,277,528 + BWI76_RS20435 0.19 -2.1 4,278,248 - BWI76_RS20435 0.85 -2.8 4,278,348 - -3.0 4,278,588 - BWI76_RS20440 0.15 -1.2 4,278,639 + BWI76_RS20440 0.20 -1.5 4,278,867 + BWI76_RS20440 0.45 -1.8 4,278,867 + BWI76_RS20440 0.45 -0.9 4,279,395 - -1.2 4,279,396 - -0.3
Or see this region's nucleotide sequence