Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS19885

Experiment: 2-Deoxyadenosine 4 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS19880 and BWI76_RS19885 are separated by 168 nucleotidesBWI76_RS19885 and BWI76_RS19890 are separated by 76 nucleotidesBWI76_RS19890 and BWI76_RS19895 overlap by 1 nucleotides BWI76_RS19880: BWI76_RS19880 - magnesium transporter, at 4,156,788 to 4,158,224 _RS19880 BWI76_RS19885: BWI76_RS19885 - multidrug ABC transporter permease/ATP-binding protein, at 4,158,393 to 4,160,036 _RS19885 BWI76_RS19890: BWI76_RS19890 - alpha-ketoglutarate-dependent dioxygenase AlkB, at 4,160,113 to 4,160,763 _RS19890 BWI76_RS19895: BWI76_RS19895 - bifunctional transcriptional regulator/O6-methylguanine-DNA methyltransferase, at 4,160,763 to 4,161,827 _RS19895 Position (kb) 4158 4159 4160 4161Strain fitness (log2 ratio) -3 -2 -1 0 1at 4157.562 kb on - strand, within BWI76_RS19880at 4157.627 kb on - strand, within BWI76_RS19880at 4157.835 kb on + strand, within BWI76_RS19880at 4158.989 kb on - strand, within BWI76_RS19885at 4159.072 kb on - strand, within BWI76_RS19885at 4159.167 kb on + strand, within BWI76_RS19885at 4159.190 kb on - strand, within BWI76_RS19885at 4159.909 kb on + strandat 4159.943 kb on + strandat 4159.951 kb on - strandat 4159.951 kb on - strandat 4159.951 kb on - strandat 4159.963 kb on - strandat 4160.078 kb on - strandat 4160.201 kb on - strand, within BWI76_RS19890at 4160.349 kb on + strand, within BWI76_RS19890at 4160.604 kb on - strand, within BWI76_RS19890at 4160.724 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C)
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4,157,562 - BWI76_RS19880 0.54 +1.1
4,157,627 - BWI76_RS19880 0.58 +0.8
4,157,835 + BWI76_RS19880 0.73 -1.4
4,158,989 - BWI76_RS19885 0.36 +0.7
4,159,072 - BWI76_RS19885 0.41 +1.1
4,159,167 + BWI76_RS19885 0.47 -0.8
4,159,190 - BWI76_RS19885 0.48 +1.7
4,159,909 + -2.6
4,159,943 + -1.1
4,159,951 - +0.6
4,159,951 - -1.3
4,159,951 - +1.3
4,159,963 - +0.6
4,160,078 - +1.3
4,160,201 - BWI76_RS19890 0.14 -2.8
4,160,349 + BWI76_RS19890 0.36 +0.4
4,160,604 - BWI76_RS19890 0.75 +0.0
4,160,724 + -1.2

Or see this region's nucleotide sequence