Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS19585

Experiment: 2-Deoxyadenosine 4 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS19575 and BWI76_RS19580 are separated by 8 nucleotidesBWI76_RS19580 and BWI76_RS19585 are separated by 24 nucleotidesBWI76_RS19585 and BWI76_RS19590 are separated by 11 nucleotides BWI76_RS19575: BWI76_RS19575 - maleylacetoacetate isomerase, at 4,092,995 to 4,093,639 _RS19575 BWI76_RS19580: BWI76_RS19580 - FAA-hydrolase, at 4,093,648 to 4,094,346 _RS19580 BWI76_RS19585: BWI76_RS19585 - gentisate 1,2-dioxygenase, at 4,094,371 to 4,095,408 _RS19585 BWI76_RS19590: BWI76_RS19590 - MFS transporter, at 4,095,420 to 4,096,778 _RS19590 Position (kb) 4094 4095 4096Strain fitness (log2 ratio) -1 0 1 2at 4093.886 kb on - strand, within BWI76_RS19580at 4093.996 kb on + strand, within BWI76_RS19580at 4094.384 kb on + strandat 4094.384 kb on + strandat 4094.553 kb on - strand, within BWI76_RS19585at 4094.733 kb on - strand, within BWI76_RS19585at 4094.905 kb on - strand, within BWI76_RS19585at 4095.479 kb on + strandat 4095.500 kb on + strandat 4095.641 kb on - strand, within BWI76_RS19590at 4095.812 kb on - strand, within BWI76_RS19590at 4096.395 kb on - strand, within BWI76_RS19590

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C)
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4,093,886 - BWI76_RS19580 0.34 +0.9
4,093,996 + BWI76_RS19580 0.50 +0.2
4,094,384 + +0.4
4,094,384 + +2.1
4,094,553 - BWI76_RS19585 0.18 -1.3
4,094,733 - BWI76_RS19585 0.35 -1.7
4,094,905 - BWI76_RS19585 0.51 -1.0
4,095,479 + -0.2
4,095,500 + +0.1
4,095,641 - BWI76_RS19590 0.16 -0.9
4,095,812 - BWI76_RS19590 0.29 +1.4
4,096,395 - BWI76_RS19590 0.72 +0.4

Or see this region's nucleotide sequence