Experiment: 2-Deoxyadenosine 4 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS18940 and BWI76_RS18945 overlap by 1 nucleotides BWI76_RS18945 and BWI76_RS18950 overlap by 19 nucleotides BWI76_RS18950 and BWI76_RS18955 overlap by 7 nucleotides BWI76_RS18955 and BWI76_RS18960 are separated by 83 nucleotides
BWI76_RS18940: BWI76_RS18940 - imidazole glycerol phosphate synthase subunit HisH, at 3,928,964 to 3,929,554
_RS18940
BWI76_RS18945: BWI76_RS18945 - 1-(5-phosphoribosyl)-5-((5- phosphoribosylamino)methylideneamino)imidazole-4- carboxamide isomerase, at 3,929,554 to 3,930,291
_RS18945
BWI76_RS18950: BWI76_RS18950 - imidazole glycerol phosphate synthase cyclase subunit, at 3,930,273 to 3,931,049
_RS18950
BWI76_RS18955: BWI76_RS18955 - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase, at 3,931,043 to 3,931,642
_RS18955
BWI76_RS18960: BWI76_RS18960 - glycosyl transferase, at 3,931,726 to 3,933,045
_RS18960
Position (kb)
3930
3931
3932 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3929.368 kb on - strand, within BWI76_RS18940 at 3929.441 kb on + strand, within BWI76_RS18940 at 3929.476 kb on + strand, within BWI76_RS18940 at 3930.057 kb on + strand, within BWI76_RS18945 at 3930.223 kb on - strand at 3930.377 kb on + strand, within BWI76_RS18950 at 3930.426 kb on + strand, within BWI76_RS18950 at 3930.445 kb on + strand, within BWI76_RS18950 at 3930.909 kb on + strand, within BWI76_RS18950 at 3931.121 kb on + strand, within BWI76_RS18955 at 3931.498 kb on - strand, within BWI76_RS18955 at 3931.509 kb on + strand, within BWI76_RS18955 at 3931.517 kb on + strand, within BWI76_RS18955 at 3931.517 kb on + strand, within BWI76_RS18955 at 3931.640 kb on - strand at 3931.653 kb on - strand at 3931.674 kb on + strand at 3931.723 kb on - strand at 3931.762 kb on + strand at 3931.770 kb on - strand at 3931.816 kb on - strand at 3931.816 kb on - strand at 3931.910 kb on - strand, within BWI76_RS18960 at 3931.936 kb on + strand, within BWI76_RS18960 at 3932.013 kb on + strand, within BWI76_RS18960 at 3932.036 kb on + strand, within BWI76_RS18960 at 3932.045 kb on - strand, within BWI76_RS18960
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C) remove 3,929,368 - BWI76_RS18940 0.68 -2.1 3,929,441 + BWI76_RS18940 0.81 -3.4 3,929,476 + BWI76_RS18940 0.87 -2.8 3,930,057 + BWI76_RS18945 0.68 -0.6 3,930,223 - -2.3 3,930,377 + BWI76_RS18950 0.13 -5.6 3,930,426 + BWI76_RS18950 0.20 -2.9 3,930,445 + BWI76_RS18950 0.22 -2.5 3,930,909 + BWI76_RS18950 0.82 -2.7 3,931,121 + BWI76_RS18955 0.13 -3.2 3,931,498 - BWI76_RS18955 0.76 -5.4 3,931,509 + BWI76_RS18955 0.78 +0.0 3,931,517 + BWI76_RS18955 0.79 -2.9 3,931,517 + BWI76_RS18955 0.79 -2.8 3,931,640 - -0.8 3,931,653 - -0.3 3,931,674 + +0.8 3,931,723 - +0.4 3,931,762 + +1.4 3,931,770 - -1.5 3,931,816 - -2.2 3,931,816 - +0.4 3,931,910 - BWI76_RS18960 0.14 +1.7 3,931,936 + BWI76_RS18960 0.16 -2.7 3,932,013 + BWI76_RS18960 0.22 -1.1 3,932,036 + BWI76_RS18960 0.23 +1.0 3,932,045 - BWI76_RS18960 0.24 -0.0
Or see this region's nucleotide sequence