Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS18600

Experiment: 2-Deoxyadenosine 4 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS18590 and BWI76_RS18595 are separated by 139 nucleotidesBWI76_RS18595 and BWI76_RS18600 are separated by 14 nucleotidesBWI76_RS18600 and BWI76_RS18605 overlap by 11 nucleotidesBWI76_RS18605 and BWI76_RS18610 are separated by 178 nucleotides BWI76_RS18590: BWI76_RS18590 - DNA-binding response regulator, at 3,855,903 to 3,856,571 _RS18590 BWI76_RS18595: BWI76_RS18595 - hypothetical protein, at 3,856,711 to 3,857,337 _RS18595 BWI76_RS18600: BWI76_RS18600 - HupC/HyaC/HydC family protein, at 3,857,352 to 3,857,960 _RS18600 BWI76_RS18605: BWI76_RS18605 - oxidoreductase molybdopterin-binding protein, at 3,857,950 to 3,858,717 _RS18605 BWI76_RS18610: BWI76_RS18610 - transcriptional regulator LysR, at 3,858,896 to 3,859,882 _RS18610 Position (kb) 3857 3858Strain fitness (log2 ratio) -3 -2 -1 0 1at 3856.412 kb on + strand, within BWI76_RS18590at 3856.420 kb on - strand, within BWI76_RS18590at 3856.516 kb on - strandat 3856.517 kb on - strandat 3856.608 kb on - strandat 3856.655 kb on + strandat 3856.663 kb on - strandat 3856.663 kb on - strandat 3856.778 kb on - strand, within BWI76_RS18595at 3856.823 kb on - strand, within BWI76_RS18595at 3857.059 kb on + strand, within BWI76_RS18595at 3857.237 kb on - strand, within BWI76_RS18595at 3857.384 kb on - strandat 3857.398 kb on - strandat 3857.416 kb on - strand, within BWI76_RS18600at 3857.540 kb on + strand, within BWI76_RS18600at 3857.572 kb on - strand, within BWI76_RS18600at 3857.585 kb on + strand, within BWI76_RS18600at 3857.676 kb on - strand, within BWI76_RS18600at 3857.737 kb on - strand, within BWI76_RS18600at 3857.795 kb on + strand, within BWI76_RS18600at 3858.065 kb on + strand, within BWI76_RS18605at 3858.180 kb on + strand, within BWI76_RS18605at 3858.189 kb on - strand, within BWI76_RS18605at 3858.685 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C)
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3,856,412 + BWI76_RS18590 0.76 +0.4
3,856,420 - BWI76_RS18590 0.77 +0.9
3,856,516 - +1.0
3,856,517 - -0.3
3,856,608 - -1.7
3,856,655 + -0.5
3,856,663 - +0.3
3,856,663 - -0.3
3,856,778 - BWI76_RS18595 0.11 +0.3
3,856,823 - BWI76_RS18595 0.18 -0.5
3,857,059 + BWI76_RS18595 0.56 -0.5
3,857,237 - BWI76_RS18595 0.84 -0.5
3,857,384 - -2.6
3,857,398 - +0.5
3,857,416 - BWI76_RS18600 0.11 -1.2
3,857,540 + BWI76_RS18600 0.31 +1.6
3,857,572 - BWI76_RS18600 0.36 +0.3
3,857,585 + BWI76_RS18600 0.38 +1.1
3,857,676 - BWI76_RS18600 0.53 -1.0
3,857,737 - BWI76_RS18600 0.63 +0.6
3,857,795 + BWI76_RS18600 0.73 +1.6
3,858,065 + BWI76_RS18605 0.15 -2.6
3,858,180 + BWI76_RS18605 0.30 -2.8
3,858,189 - BWI76_RS18605 0.31 -1.0
3,858,685 + +0.4

Or see this region's nucleotide sequence