Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS17190

Experiment: 2-Deoxyadenosine 4 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS17180 and BWI76_RS17185 are separated by 106 nucleotidesBWI76_RS17185 and BWI76_RS17190 are separated by 19 nucleotidesBWI76_RS17190 and BWI76_RS17195 are separated by 211 nucleotidesBWI76_RS17195 and BWI76_RS17200 are separated by 58 nucleotidesBWI76_RS17200 and BWI76_RS17205 are separated by 32 nucleotides BWI76_RS17180: BWI76_RS17180 - hypothetical protein, at 3,561,034 to 3,561,318 _RS17180 BWI76_RS17185: BWI76_RS17185 - fructosamine kinase family protein, at 3,561,425 to 3,562,297 _RS17185 BWI76_RS17190: BWI76_RS17190 - TonB system transport protein TonB, at 3,562,317 to 3,563,054 _RS17190 BWI76_RS17195: BWI76_RS17195 - hypothetical protein, at 3,563,266 to 3,563,562 _RS17195 BWI76_RS17200: BWI76_RS17200 - hypothetical protein, at 3,563,621 to 3,563,905 _RS17200 BWI76_RS17205: BWI76_RS17205 - cardiolipin synthase A, at 3,563,938 to 3,565,398 _RS17205 Position (kb) 3562 3563 3564Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3561.767 kb on + strand, within BWI76_RS17185at 3562.198 kb on + strand, within BWI76_RS17185at 3562.548 kb on + strand, within BWI76_RS17190at 3562.960 kb on - strand, within BWI76_RS17190at 3562.965 kb on - strand, within BWI76_RS17190at 3563.466 kb on + strand, within BWI76_RS17195at 3563.639 kb on + strandat 3563.776 kb on - strand, within BWI76_RS17200at 3563.964 kb on - strandat 3563.964 kb on - strandat 3563.978 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C)
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3,561,767 + BWI76_RS17185 0.39 +0.1
3,562,198 + BWI76_RS17185 0.89 +0.7
3,562,548 + BWI76_RS17190 0.31 -2.9
3,562,960 - BWI76_RS17190 0.87 -3.5
3,562,965 - BWI76_RS17190 0.88 -1.8
3,563,466 + BWI76_RS17195 0.67 -1.0
3,563,639 + +0.1
3,563,776 - BWI76_RS17200 0.54 -0.1
3,563,964 - -0.5
3,563,964 - +0.9
3,563,978 - +1.9

Or see this region's nucleotide sequence