Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS14145

Experiment: 2-Deoxyadenosine 4 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS14140 and BWI76_RS14145 are separated by 43 nucleotidesBWI76_RS14145 and BWI76_RS14150 overlap by 17 nucleotides BWI76_RS14140: BWI76_RS14140 - glycogen debranching enzyme, at 2,956,486 to 2,958,561 _RS14140 BWI76_RS14145: BWI76_RS14145 - dihydrolipoyl dehydrogenase, at 2,958,605 to 2,960,002 _RS14145 BWI76_RS14150: BWI76_RS14150 - dihydrolipoyllysine-residue acetyltransferase, at 2,959,986 to 2,961,515 _RS14150 Position (kb) 2958 2959 2960 2961Strain fitness (log2 ratio) -2 -1 0 1 2at 2957.702 kb on - strand, within BWI76_RS14140at 2957.797 kb on - strand, within BWI76_RS14140at 2957.866 kb on + strand, within BWI76_RS14140at 2957.966 kb on + strand, within BWI76_RS14140at 2958.126 kb on - strand, within BWI76_RS14140at 2958.178 kb on - strand, within BWI76_RS14140at 2958.296 kb on + strand, within BWI76_RS14140at 2958.322 kb on + strand, within BWI76_RS14140at 2958.462 kb on - strandat 2958.700 kb on + strandat 2958.700 kb on + strandat 2958.743 kb on - strandat 2958.830 kb on - strand, within BWI76_RS14145at 2959.053 kb on + strand, within BWI76_RS14145at 2959.278 kb on + strand, within BWI76_RS14145at 2959.758 kb on - strand, within BWI76_RS14145at 2960.174 kb on - strand, within BWI76_RS14150at 2960.241 kb on - strand, within BWI76_RS14150

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C)
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2,957,702 - BWI76_RS14140 0.59 +0.2
2,957,797 - BWI76_RS14140 0.63 -0.8
2,957,866 + BWI76_RS14140 0.66 -0.2
2,957,966 + BWI76_RS14140 0.71 +0.3
2,958,126 - BWI76_RS14140 0.79 +1.4
2,958,178 - BWI76_RS14140 0.82 -2.4
2,958,296 + BWI76_RS14140 0.87 -0.5
2,958,322 + BWI76_RS14140 0.88 -0.2
2,958,462 - +0.7
2,958,700 + +2.0
2,958,700 + +1.9
2,958,743 - +0.3
2,958,830 - BWI76_RS14145 0.16 +1.4
2,959,053 + BWI76_RS14145 0.32 +0.4
2,959,278 + BWI76_RS14145 0.48 +0.5
2,959,758 - BWI76_RS14145 0.82 +0.5
2,960,174 - BWI76_RS14150 0.12 -0.3
2,960,241 - BWI76_RS14150 0.17 -0.8

Or see this region's nucleotide sequence