Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS13980

Experiment: 2-Deoxyadenosine 4 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS13970 and BWI76_RS13975 are separated by 4 nucleotidesBWI76_RS13975 and BWI76_RS13980 are separated by 10 nucleotidesBWI76_RS13980 and BWI76_RS13985 are separated by 168 nucleotides BWI76_RS13970: BWI76_RS13970 - ABC transporter, at 2,915,967 to 2,916,743 _RS13970 BWI76_RS13975: BWI76_RS13975 - urea carboxylase, at 2,916,748 to 2,917,476 _RS13975 BWI76_RS13980: BWI76_RS13980 - hypothetical protein, at 2,917,487 to 2,918,122 _RS13980 BWI76_RS13985: BWI76_RS13985 - urea carboxylase, at 2,918,291 to 2,921,896 _RS13985 Position (kb) 2917 2918 2919Strain fitness (log2 ratio) -2 -1 0 1 2at 2916.576 kb on + strand, within BWI76_RS13970at 2916.777 kb on - strandat 2916.778 kb on - strandat 2917.061 kb on - strand, within BWI76_RS13975at 2917.396 kb on + strand, within BWI76_RS13975at 2917.845 kb on - strand, within BWI76_RS13980at 2917.850 kb on + strand, within BWI76_RS13980at 2918.124 kb on - strandat 2918.187 kb on - strandat 2918.342 kb on + strandat 2918.404 kb on - strandat 2918.506 kb on - strandat 2918.564 kb on + strandat 2918.955 kb on + strand, within BWI76_RS13985at 2919.073 kb on + strand, within BWI76_RS13985

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C)
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2,916,576 + BWI76_RS13970 0.78 +0.3
2,916,777 - +0.4
2,916,778 - +0.3
2,917,061 - BWI76_RS13975 0.43 +0.3
2,917,396 + BWI76_RS13975 0.89 +0.2
2,917,845 - BWI76_RS13980 0.56 -1.3
2,917,850 + BWI76_RS13980 0.57 -1.7
2,918,124 - +0.3
2,918,187 - +0.1
2,918,342 + +0.5
2,918,404 - +0.3
2,918,506 - -0.2
2,918,564 + +1.8
2,918,955 + BWI76_RS13985 0.18 +0.5
2,919,073 + BWI76_RS13985 0.22 -2.3

Or see this region's nucleotide sequence