Experiment: 2-Deoxyadenosine 4 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13310 and BWI76_RS13315 are separated by 75 nucleotides BWI76_RS13315 and BWI76_RS13320 are separated by 95 nucleotides BWI76_RS13320 and BWI76_RS13325 are separated by 110 nucleotides
BWI76_RS13310: BWI76_RS13310 - glucan biosynthesis protein D, at 2,775,559 to 2,777,214
_RS13310
BWI76_RS13315: BWI76_RS13315 - 50S ribosomal protein L7/L12-serine acetyltransferase, at 2,777,290 to 2,777,874
_RS13315
BWI76_RS13320: BWI76_RS13320 - putative LpxA-like enzyme, at 2,777,970 to 2,778,950
_RS13320
BWI76_RS13325: BWI76_RS13325 - dicarboxylate transporter/tellurite-resistance protein TehA, at 2,779,061 to 2,780,059
_RS13325
Position (kb)
2777
2778
2779 Strain fitness (log2 ratio)
-2
-1
0
1 at 2777.103 kb on - strand at 2777.126 kb on - strand at 2777.128 kb on - strand at 2777.367 kb on - strand, within BWI76_RS13315 at 2778.638 kb on + strand, within BWI76_RS13320 at 2778.797 kb on - strand, within BWI76_RS13320 at 2778.887 kb on + strand at 2778.887 kb on + strand at 2778.901 kb on + strand at 2779.143 kb on + strand at 2779.202 kb on + strand, within BWI76_RS13325 at 2779.278 kb on + strand, within BWI76_RS13325 at 2779.286 kb on - strand, within BWI76_RS13325 at 2779.346 kb on - strand, within BWI76_RS13325 at 2779.605 kb on + strand, within BWI76_RS13325
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C) remove 2,777,103 - +0.1 2,777,126 - +0.6 2,777,128 - -2.2 2,777,367 - BWI76_RS13315 0.13 +0.6 2,778,638 + BWI76_RS13320 0.68 -1.1 2,778,797 - BWI76_RS13320 0.84 -1.4 2,778,887 + -0.2 2,778,887 + +0.1 2,778,901 + -1.7 2,779,143 + -0.6 2,779,202 + BWI76_RS13325 0.14 -0.1 2,779,278 + BWI76_RS13325 0.22 +0.5 2,779,286 - BWI76_RS13325 0.23 -0.3 2,779,346 - BWI76_RS13325 0.29 -1.1 2,779,605 + BWI76_RS13325 0.54 -1.1
Or see this region's nucleotide sequence