Experiment: 2-Deoxyadenosine 4 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12650 and BWI76_RS12655 are separated by 56 nucleotides BWI76_RS12655 and BWI76_RS12660 are separated by 177 nucleotides BWI76_RS12660 and BWI76_RS12665 are separated by 154 nucleotides BWI76_RS12665 and BWI76_RS12670 are separated by 275 nucleotides BWI76_RS12670 and BWI76_RS12675 overlap by 1 nucleotides
BWI76_RS12650: BWI76_RS12650 - membrane protein, at 2,639,709 to 2,640,272
_RS12650
BWI76_RS12655: BWI76_RS12655 - PTS cellobiose transporter subunit IIB, at 2,640,329 to 2,640,631
_RS12655
BWI76_RS12660: BWI76_RS12660 - hypothetical protein, at 2,640,809 to 2,641,000
_RS12660
BWI76_RS12665: BWI76_RS12665 - hypothetical protein, at 2,641,155 to 2,641,349
_RS12665
BWI76_RS12670: BWI76_RS12670 - DNA polymerase V subunit UmuD, at 2,641,625 to 2,642,047
_RS12670
BWI76_RS12675: BWI76_RS12675 - putative UV protection and mutation protein, at 2,642,047 to 2,643,312
_RS12675
Position (kb)
2641
2642 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2640.187 kb on + strand, within BWI76_RS12650 at 2640.210 kb on + strand, within BWI76_RS12650 at 2640.220 kb on + strand at 2640.226 kb on + strand at 2640.227 kb on + strand at 2640.249 kb on + strand at 2640.280 kb on + strand at 2640.349 kb on - strand at 2640.352 kb on + strand at 2640.360 kb on - strand, within BWI76_RS12655 at 2640.369 kb on - strand, within BWI76_RS12655 at 2640.394 kb on - strand, within BWI76_RS12655 at 2640.410 kb on + strand, within BWI76_RS12655 at 2640.410 kb on + strand, within BWI76_RS12655 at 2640.410 kb on + strand, within BWI76_RS12655 at 2640.410 kb on + strand, within BWI76_RS12655 at 2640.418 kb on + strand, within BWI76_RS12655 at 2640.418 kb on - strand, within BWI76_RS12655 at 2640.418 kb on - strand, within BWI76_RS12655 at 2640.418 kb on - strand, within BWI76_RS12655 at 2640.418 kb on - strand, within BWI76_RS12655 at 2640.418 kb on - strand, within BWI76_RS12655 at 2640.418 kb on - strand, within BWI76_RS12655 at 2640.418 kb on - strand, within BWI76_RS12655 at 2640.418 kb on - strand, within BWI76_RS12655 at 2640.418 kb on - strand, within BWI76_RS12655 at 2640.418 kb on - strand at 2640.418 kb on - strand, within BWI76_RS12655 at 2640.418 kb on - strand, within BWI76_RS12655 at 2640.418 kb on - strand, within BWI76_RS12655 at 2640.425 kb on - strand, within BWI76_RS12655 at 2640.427 kb on - strand, within BWI76_RS12655 at 2640.447 kb on + strand, within BWI76_RS12655 at 2640.450 kb on - strand at 2640.455 kb on - strand, within BWI76_RS12655 at 2640.455 kb on - strand, within BWI76_RS12655 at 2640.455 kb on - strand, within BWI76_RS12655 at 2640.455 kb on - strand, within BWI76_RS12655 at 2640.455 kb on - strand, within BWI76_RS12655 at 2640.633 kb on + strand at 2640.641 kb on - strand at 2640.641 kb on - strand at 2640.641 kb on - strand at 2640.641 kb on - strand at 2640.661 kb on + strand at 2640.661 kb on + strand at 2640.747 kb on + strand at 2640.781 kb on + strand at 2640.784 kb on + strand at 2640.784 kb on - strand at 2640.834 kb on - strand, within BWI76_RS12660 at 2640.836 kb on - strand, within BWI76_RS12660 at 2640.836 kb on - strand, within BWI76_RS12660 at 2640.851 kb on + strand, within BWI76_RS12660 at 2640.851 kb on + strand, within BWI76_RS12660 at 2640.857 kb on - strand, within BWI76_RS12660 at 2640.857 kb on - strand, within BWI76_RS12660 at 2640.857 kb on - strand, within BWI76_RS12660 at 2640.858 kb on - strand, within BWI76_RS12660 at 2640.859 kb on - strand, within BWI76_RS12660 at 2640.883 kb on - strand, within BWI76_RS12660 at 2640.958 kb on - strand, within BWI76_RS12660 at 2640.958 kb on - strand, within BWI76_RS12660 at 2640.978 kb on - strand, within BWI76_RS12660 at 2640.983 kb on + strand at 2640.985 kb on + strand at 2640.992 kb on - strand at 2640.993 kb on + strand at 2640.993 kb on + strand at 2640.993 kb on - strand at 2640.993 kb on - strand at 2640.995 kb on + strand at 2641.036 kb on - strand at 2641.036 kb on - strand at 2641.176 kb on + strand, within BWI76_RS12665 at 2641.197 kb on - strand, within BWI76_RS12665 at 2641.386 kb on - strand at 2641.386 kb on - strand at 2641.388 kb on + strand at 2641.396 kb on + strand at 2641.404 kb on - strand at 2641.423 kb on - strand at 2641.456 kb on + strand at 2641.456 kb on + strand at 2641.464 kb on - strand at 2641.808 kb on - strand, within BWI76_RS12670 at 2641.981 kb on + strand, within BWI76_RS12670 at 2642.053 kb on + strand at 2642.244 kb on + strand, within BWI76_RS12675
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C) remove 2,640,187 + BWI76_RS12650 0.85 +1.2 2,640,210 + BWI76_RS12650 0.89 -2.0 2,640,220 + -1.6 2,640,226 + +1.5 2,640,227 + -1.4 2,640,249 + -0.2 2,640,280 + +1.1 2,640,349 - -0.9 2,640,352 + +0.5 2,640,360 - BWI76_RS12655 0.10 +2.1 2,640,369 - BWI76_RS12655 0.13 -1.6 2,640,394 - BWI76_RS12655 0.21 +2.0 2,640,410 + BWI76_RS12655 0.27 +0.8 2,640,410 + BWI76_RS12655 0.27 -4.0 2,640,410 + BWI76_RS12655 0.27 -0.0 2,640,410 + BWI76_RS12655 0.27 +1.1 2,640,418 + BWI76_RS12655 0.29 +0.4 2,640,418 - BWI76_RS12655 0.29 +0.4 2,640,418 - BWI76_RS12655 0.29 +0.8 2,640,418 - BWI76_RS12655 0.29 +1.9 2,640,418 - BWI76_RS12655 0.29 +0.2 2,640,418 - BWI76_RS12655 0.29 -1.0 2,640,418 - BWI76_RS12655 0.29 +2.2 2,640,418 - BWI76_RS12655 0.29 -0.7 2,640,418 - BWI76_RS12655 0.29 +0.3 2,640,418 - BWI76_RS12655 0.29 +0.4 2,640,418 - -2.2 2,640,418 - BWI76_RS12655 0.29 +1.0 2,640,418 - BWI76_RS12655 0.29 +3.5 2,640,418 - BWI76_RS12655 0.29 +1.6 2,640,425 - BWI76_RS12655 0.32 +0.1 2,640,427 - BWI76_RS12655 0.32 -4.0 2,640,447 + BWI76_RS12655 0.39 -1.8 2,640,450 - +1.5 2,640,455 - BWI76_RS12655 0.42 -1.0 2,640,455 - BWI76_RS12655 0.42 +0.4 2,640,455 - BWI76_RS12655 0.42 -0.8 2,640,455 - BWI76_RS12655 0.42 -2.5 2,640,455 - BWI76_RS12655 0.42 -2.0 2,640,633 + +1.6 2,640,641 - +1.0 2,640,641 - +1.0 2,640,641 - +1.0 2,640,641 - -2.0 2,640,661 + +0.5 2,640,661 + -2.4 2,640,747 + -0.5 2,640,781 + -1.8 2,640,784 + +0.3 2,640,784 - -1.0 2,640,834 - BWI76_RS12660 0.13 -0.9 2,640,836 - BWI76_RS12660 0.14 +1.5 2,640,836 - BWI76_RS12660 0.14 +1.0 2,640,851 + BWI76_RS12660 0.22 +0.1 2,640,851 + BWI76_RS12660 0.22 -0.2 2,640,857 - BWI76_RS12660 0.25 -1.5 2,640,857 - BWI76_RS12660 0.25 +0.0 2,640,857 - BWI76_RS12660 0.25 +0.4 2,640,858 - BWI76_RS12660 0.26 +0.4 2,640,859 - BWI76_RS12660 0.26 +0.9 2,640,883 - BWI76_RS12660 0.39 -0.4 2,640,958 - BWI76_RS12660 0.78 +0.4 2,640,958 - BWI76_RS12660 0.78 -0.1 2,640,978 - BWI76_RS12660 0.88 +1.0 2,640,983 + -0.0 2,640,985 + +0.5 2,640,992 - +0.1 2,640,993 + -1.8 2,640,993 + -1.0 2,640,993 - -1.6 2,640,993 - +1.0 2,640,995 + +0.3 2,641,036 - +0.9 2,641,036 - -0.6 2,641,176 + BWI76_RS12665 0.11 -0.1 2,641,197 - BWI76_RS12665 0.22 +1.6 2,641,386 - +1.2 2,641,386 - +0.0 2,641,388 + -0.5 2,641,396 + -1.5 2,641,404 - -2.7 2,641,423 - -0.5 2,641,456 + +1.2 2,641,456 + +0.8 2,641,464 - -0.4 2,641,808 - BWI76_RS12670 0.43 +0.9 2,641,981 + BWI76_RS12670 0.84 -0.5 2,642,053 + -0.1 2,642,244 + BWI76_RS12675 0.16 -0.4
Or see this region's nucleotide sequence