Experiment: 2-Deoxyadenosine 4 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11425 and BWI76_RS11430 are separated by 297 nucleotides BWI76_RS11430 and BWI76_RS11435 overlap by 6 nucleotides BWI76_RS11435 and BWI76_RS11440 overlap by 14 nucleotides BWI76_RS11440 and BWI76_RS11445 are separated by 162 nucleotides
BWI76_RS11425: BWI76_RS11425 - GAF domain/diguanylate cyclase, at 2,406,404 to 2,407,417
_RS11425
BWI76_RS11430: BWI76_RS11430 - lipoprotein, at 2,407,715 to 2,407,954
_RS11430
BWI76_RS11435: BWI76_RS11435 - hypothetical protein, at 2,407,949 to 2,408,293
_RS11435
BWI76_RS11440: BWI76_RS11440 - hypothetical protein, at 2,408,280 to 2,408,789
_RS11440
BWI76_RS11445: BWI76_RS11445 - hypothetical protein, at 2,408,952 to 2,409,644
_RS11445
Position (kb)
2407
2408
2409 Strain fitness (log2 ratio)
-1
0
1 at 2407.016 kb on - strand, within BWI76_RS11425 at 2407.252 kb on - strand, within BWI76_RS11425 at 2407.602 kb on + strand at 2407.722 kb on + strand at 2407.750 kb on + strand, within BWI76_RS11430 at 2408.018 kb on - strand, within BWI76_RS11435 at 2408.109 kb on - strand, within BWI76_RS11435 at 2408.257 kb on + strand, within BWI76_RS11435 at 2408.469 kb on + strand, within BWI76_RS11440 at 2408.829 kb on - strand at 2408.949 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C) remove 2,407,016 - BWI76_RS11425 0.60 -0.2 2,407,252 - BWI76_RS11425 0.84 +0.5 2,407,602 + -0.4 2,407,722 + -0.1 2,407,750 + BWI76_RS11430 0.15 -0.3 2,408,018 - BWI76_RS11435 0.20 -1.6 2,408,109 - BWI76_RS11435 0.46 +1.2 2,408,257 + BWI76_RS11435 0.89 +0.8 2,408,469 + BWI76_RS11440 0.37 +0.6 2,408,829 - -0.5 2,408,949 + +0.1
Or see this region's nucleotide sequence