Experiment: 2-Deoxyadenosine 4 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11245 and BWI76_RS11250 overlap by 4 nucleotides BWI76_RS11250 and BWI76_RS11255 overlap by 17 nucleotides BWI76_RS11255 and BWI76_RS11260 overlap by 22 nucleotides BWI76_RS11260 and BWI76_RS11265 are separated by 19 nucleotides
BWI76_RS11245: BWI76_RS11245 - spermidine/putrescine ABC transporter permease PotC, at 2,372,250 to 2,373,035
_RS11245
BWI76_RS11250: BWI76_RS11250 - spermidine/putrescine ABC transporter permease PotB, at 2,373,032 to 2,373,889
_RS11250
BWI76_RS11255: BWI76_RS11255 - putrescine/spermidine ABC transporter ATP-binding protein, at 2,373,873 to 2,375,009
_RS11255
BWI76_RS11260: BWI76_RS11260 - hypothetical protein, at 2,374,988 to 2,375,247
_RS11260
BWI76_RS11265: BWI76_RS11265 - peptidase T, at 2,375,267 to 2,376,496
_RS11265
Position (kb)
2373
2374
2375
2376 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2372.922 kb on + strand, within BWI76_RS11245 at 2373.411 kb on + strand, within BWI76_RS11250 at 2373.732 kb on - strand, within BWI76_RS11250 at 2373.837 kb on - strand at 2374.067 kb on - strand, within BWI76_RS11255 at 2374.067 kb on - strand, within BWI76_RS11255 at 2374.067 kb on - strand, within BWI76_RS11255 at 2375.091 kb on + strand, within BWI76_RS11260 at 2375.650 kb on + strand, within BWI76_RS11265 at 2375.996 kb on - strand, within BWI76_RS11265
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C) remove 2,372,922 + BWI76_RS11245 0.85 -0.5 2,373,411 + BWI76_RS11250 0.44 +0.3 2,373,732 - BWI76_RS11250 0.82 -0.2 2,373,837 - -1.1 2,374,067 - BWI76_RS11255 0.17 -3.4 2,374,067 - BWI76_RS11255 0.17 -1.5 2,374,067 - BWI76_RS11255 0.17 -0.1 2,375,091 + BWI76_RS11260 0.40 -0.1 2,375,650 + BWI76_RS11265 0.31 -1.1 2,375,996 - BWI76_RS11265 0.59 -3.1
Or see this region's nucleotide sequence