Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS11255

Experiment: 2-Deoxyadenosine 4 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS11245 and BWI76_RS11250 overlap by 4 nucleotidesBWI76_RS11250 and BWI76_RS11255 overlap by 17 nucleotidesBWI76_RS11255 and BWI76_RS11260 overlap by 22 nucleotidesBWI76_RS11260 and BWI76_RS11265 are separated by 19 nucleotides BWI76_RS11245: BWI76_RS11245 - spermidine/putrescine ABC transporter permease PotC, at 2,372,250 to 2,373,035 _RS11245 BWI76_RS11250: BWI76_RS11250 - spermidine/putrescine ABC transporter permease PotB, at 2,373,032 to 2,373,889 _RS11250 BWI76_RS11255: BWI76_RS11255 - putrescine/spermidine ABC transporter ATP-binding protein, at 2,373,873 to 2,375,009 _RS11255 BWI76_RS11260: BWI76_RS11260 - hypothetical protein, at 2,374,988 to 2,375,247 _RS11260 BWI76_RS11265: BWI76_RS11265 - peptidase T, at 2,375,267 to 2,376,496 _RS11265 Position (kb) 2373 2374 2375 2376Strain fitness (log2 ratio) -3 -2 -1 0 1at 2372.922 kb on + strand, within BWI76_RS11245at 2373.411 kb on + strand, within BWI76_RS11250at 2373.732 kb on - strand, within BWI76_RS11250at 2373.837 kb on - strandat 2374.067 kb on - strand, within BWI76_RS11255at 2374.067 kb on - strand, within BWI76_RS11255at 2374.067 kb on - strand, within BWI76_RS11255at 2375.091 kb on + strand, within BWI76_RS11260at 2375.650 kb on + strand, within BWI76_RS11265at 2375.996 kb on - strand, within BWI76_RS11265

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C)
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2,372,922 + BWI76_RS11245 0.85 -0.5
2,373,411 + BWI76_RS11250 0.44 +0.3
2,373,732 - BWI76_RS11250 0.82 -0.2
2,373,837 - -1.1
2,374,067 - BWI76_RS11255 0.17 -3.4
2,374,067 - BWI76_RS11255 0.17 -1.5
2,374,067 - BWI76_RS11255 0.17 -0.1
2,375,091 + BWI76_RS11260 0.40 -0.1
2,375,650 + BWI76_RS11265 0.31 -1.1
2,375,996 - BWI76_RS11265 0.59 -3.1

Or see this region's nucleotide sequence