Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS10735

Experiment: 2-Deoxyadenosine 4 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS10730 and BWI76_RS10735 are separated by 147 nucleotidesBWI76_RS10735 and BWI76_RS10740 are separated by 19 nucleotidesBWI76_RS10740 and BWI76_RS10745 are separated by 374 nucleotides BWI76_RS10730: BWI76_RS10730 - bifunctional glucose-1-phosphatase/inositol phosphatase, at 2,267,463 to 2,268,710 _RS10730 BWI76_RS10735: BWI76_RS10735 - hypothetical protein, at 2,268,858 to 2,269,085 _RS10735 BWI76_RS10740: BWI76_RS10740 - NAD(P)H:quinone oxidoreductase, at 2,269,105 to 2,269,701 _RS10740 BWI76_RS10745: BWI76_RS10745 - hypothetical protein, at 2,270,076 to 2,270,249 _RS10745 Position (kb) 2268 2269 2270Strain fitness (log2 ratio) -1 0 1at 2267.861 kb on - strand, within BWI76_RS10730at 2268.911 kb on - strand, within BWI76_RS10735at 2269.145 kb on + strandat 2269.145 kb on + strandat 2269.610 kb on - strand, within BWI76_RS10740at 2269.634 kb on - strand, within BWI76_RS10740at 2269.652 kb on + strandat 2269.881 kb on - strandat 2269.988 kb on - strandat 2270.016 kb on + strandat 2270.024 kb on - strandat 2270.035 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C)
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2,267,861 - BWI76_RS10730 0.32 -0.3
2,268,911 - BWI76_RS10735 0.23 +1.0
2,269,145 + -0.1
2,269,145 + +0.3
2,269,610 - BWI76_RS10740 0.85 +0.6
2,269,634 - BWI76_RS10740 0.89 +0.1
2,269,652 + -0.6
2,269,881 - -0.7
2,269,988 - -0.1
2,270,016 + -0.9
2,270,024 - -1.0
2,270,035 + -0.3

Or see this region's nucleotide sequence