Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS10575

Experiment: 2-Deoxyadenosine 4 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS10570 and BWI76_RS10575 are separated by 9 nucleotidesBWI76_RS10575 and BWI76_RS10580 overlap by 1 nucleotidesBWI76_RS10580 and BWI76_RS10585 overlap by 20 nucleotides BWI76_RS10570: BWI76_RS10570 - cysteine ABC transporter substrate-binding protein, at 2,232,870 to 2,233,655 _RS10570 BWI76_RS10575: BWI76_RS10575 - polar amino acid ABC transporter permease, at 2,233,665 to 2,234,330 _RS10575 BWI76_RS10580: BWI76_RS10580 - amino acid ABC transporter permease, at 2,234,330 to 2,234,986 _RS10580 BWI76_RS10585: BWI76_RS10585 - amino acid ABC transporter ATP-binding protein, at 2,234,967 to 2,235,707 _RS10585 Position (kb) 2233 2234 2235Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2232.893 kb on + strandat 2232.893 kb on + strandat 2233.032 kb on - strand, within BWI76_RS10570at 2233.157 kb on - strand, within BWI76_RS10570at 2233.221 kb on - strand, within BWI76_RS10570at 2233.384 kb on - strand, within BWI76_RS10570at 2233.713 kb on - strandat 2233.851 kb on - strand, within BWI76_RS10575at 2233.978 kb on + strand, within BWI76_RS10575at 2234.275 kb on - strandat 2234.506 kb on + strand, within BWI76_RS10580at 2234.514 kb on - strand, within BWI76_RS10580at 2234.855 kb on - strand, within BWI76_RS10580at 2235.150 kb on - strandat 2235.161 kb on + strand, within BWI76_RS10585

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C)
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2,232,893 + +0.2
2,232,893 + +1.5
2,233,032 - BWI76_RS10570 0.21 +0.8
2,233,157 - BWI76_RS10570 0.37 -0.7
2,233,221 - BWI76_RS10570 0.45 -2.7
2,233,384 - BWI76_RS10570 0.65 +0.1
2,233,713 - -0.3
2,233,851 - BWI76_RS10575 0.28 +1.5
2,233,978 + BWI76_RS10575 0.47 -0.3
2,234,275 - +1.5
2,234,506 + BWI76_RS10580 0.27 +0.9
2,234,514 - BWI76_RS10580 0.28 +1.5
2,234,855 - BWI76_RS10580 0.80 +0.9
2,235,150 - +2.9
2,235,161 + BWI76_RS10585 0.26 +1.0

Or see this region's nucleotide sequence