Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS10245

Experiment: 2-Deoxyadenosine 4 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS10240 and BWI76_RS10245 are separated by 317 nucleotidesBWI76_RS10245 and BWI76_RS10250 are separated by 68 nucleotides BWI76_RS10240: BWI76_RS10240 - hypothetical protein, at 2,168,065 to 2,169,282 _RS10240 BWI76_RS10245: BWI76_RS10245 - aspartate aminotransferase, at 2,169,600 to 2,170,808 _RS10245 BWI76_RS10250: BWI76_RS10250 - glutamine amidotransferase, at 2,170,877 to 2,171,974 _RS10250 Position (kb) 2169 2170 2171Strain fitness (log2 ratio) -2 -1 0 1at 2169.491 kb on + strandat 2169.584 kb on - strandat 2169.584 kb on - strandat 2169.781 kb on - strand, within BWI76_RS10245at 2170.526 kb on + strand, within BWI76_RS10245at 2170.729 kb on + strandat 2171.157 kb on - strand, within BWI76_RS10250at 2171.184 kb on - strand, within BWI76_RS10250at 2171.448 kb on + strand, within BWI76_RS10250at 2171.456 kb on - strand, within BWI76_RS10250at 2171.456 kb on - strand, within BWI76_RS10250at 2171.552 kb on + strand, within BWI76_RS10250

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C)
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2,169,491 + -1.9
2,169,584 - -0.7
2,169,584 - +0.4
2,169,781 - BWI76_RS10245 0.15 +1.4
2,170,526 + BWI76_RS10245 0.77 +0.0
2,170,729 + +0.1
2,171,157 - BWI76_RS10250 0.26 +0.1
2,171,184 - BWI76_RS10250 0.28 +0.6
2,171,448 + BWI76_RS10250 0.52 +0.9
2,171,456 - BWI76_RS10250 0.53 +0.4
2,171,456 - BWI76_RS10250 0.53 -0.1
2,171,552 + BWI76_RS10250 0.61 +0.9

Or see this region's nucleotide sequence