Experiment: 2-Deoxyadenosine 4 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09185 and BWI76_RS09190 are separated by 79 nucleotides BWI76_RS09190 and BWI76_RS09195 overlap by 23 nucleotides BWI76_RS09195 and BWI76_RS09200 are separated by 10 nucleotides BWI76_RS09200 and BWI76_RS09205 are separated by 11 nucleotides
BWI76_RS09185: BWI76_RS09185 - integrase, at 1,939,457 to 1,940,503
_RS09185
BWI76_RS09190: BWI76_RS09190 - hypothetical protein, at 1,940,583 to 1,940,954
_RS09190
BWI76_RS09195: BWI76_RS09195 - N-acetyltransferase, at 1,940,932 to 1,942,005
_RS09195
BWI76_RS09200: BWI76_RS09200 - hypothetical protein, at 1,942,016 to 1,942,600
_RS09200
BWI76_RS09205: BWI76_RS09205 - phage repressor protein CI, at 1,942,612 to 1,943,172
_RS09205
Position (kb)
1940
1941
1942
1943 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1940.055 kb on + strand, within BWI76_RS09185 at 1940.135 kb on - strand, within BWI76_RS09185 at 1940.168 kb on - strand, within BWI76_RS09185 at 1940.278 kb on + strand, within BWI76_RS09185 at 1940.349 kb on + strand, within BWI76_RS09185 at 1940.369 kb on - strand, within BWI76_RS09185 at 1940.384 kb on - strand, within BWI76_RS09185 at 1940.427 kb on + strand at 1940.457 kb on - strand at 1940.566 kb on + strand at 1940.571 kb on + strand at 1940.579 kb on - strand at 1940.579 kb on - strand at 1940.581 kb on + strand at 1940.581 kb on - strand at 1940.589 kb on - strand at 1940.606 kb on - strand at 1940.607 kb on - strand at 1940.616 kb on - strand at 1940.616 kb on - strand at 1940.616 kb on - strand at 1940.637 kb on - strand, within BWI76_RS09190 at 1940.638 kb on - strand, within BWI76_RS09190 at 1940.674 kb on - strand, within BWI76_RS09190 at 1940.693 kb on - strand, within BWI76_RS09190 at 1940.693 kb on - strand, within BWI76_RS09190 at 1940.694 kb on - strand, within BWI76_RS09190 at 1940.772 kb on - strand, within BWI76_RS09190 at 1940.772 kb on - strand, within BWI76_RS09190 at 1940.818 kb on - strand, within BWI76_RS09190 at 1940.831 kb on - strand, within BWI76_RS09190 at 1940.863 kb on - strand, within BWI76_RS09190 at 1941.009 kb on - strand at 1941.512 kb on - strand at 1941.567 kb on - strand, within BWI76_RS09195 at 1941.882 kb on - strand, within BWI76_RS09195 at 1942.098 kb on + strand, within BWI76_RS09200 at 1942.282 kb on - strand, within BWI76_RS09200 at 1942.420 kb on + strand, within BWI76_RS09200 at 1942.420 kb on + strand, within BWI76_RS09200 at 1942.420 kb on + strand, within BWI76_RS09200 at 1942.420 kb on + strand, within BWI76_RS09200 at 1942.428 kb on - strand, within BWI76_RS09200 at 1942.428 kb on - strand, within BWI76_RS09200 at 1942.430 kb on + strand, within BWI76_RS09200 at 1942.430 kb on + strand, within BWI76_RS09200 at 1942.438 kb on - strand, within BWI76_RS09200 at 1942.438 kb on - strand, within BWI76_RS09200 at 1942.438 kb on - strand, within BWI76_RS09200 at 1942.535 kb on + strand, within BWI76_RS09200 at 1942.535 kb on + strand, within BWI76_RS09200 at 1942.543 kb on - strand at 1942.543 kb on - strand at 1942.543 kb on - strand at 1942.543 kb on - strand at 1942.545 kb on + strand at 1942.545 kb on + strand at 1942.545 kb on + strand at 1942.545 kb on + strand at 1942.545 kb on + strand at 1942.545 kb on + strand at 1942.545 kb on + strand at 1942.545 kb on - strand at 1942.553 kb on - strand at 1942.553 kb on - strand at 1942.553 kb on - strand at 1942.553 kb on - strand at 1942.553 kb on - strand at 1942.553 kb on - strand at 1942.553 kb on - strand at 1942.553 kb on - strand at 1942.553 kb on - strand at 1942.553 kb on - strand at 1942.553 kb on - strand at 1942.553 kb on - strand at 1942.553 kb on - strand at 1942.553 kb on - strand at 1942.589 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C) remove 1,940,055 + BWI76_RS09185 0.57 +0.5 1,940,135 - BWI76_RS09185 0.65 -0.4 1,940,168 - BWI76_RS09185 0.68 +0.5 1,940,278 + BWI76_RS09185 0.78 -0.2 1,940,349 + BWI76_RS09185 0.85 -1.2 1,940,369 - BWI76_RS09185 0.87 -0.1 1,940,384 - BWI76_RS09185 0.89 +0.9 1,940,427 + -0.3 1,940,457 - -0.0 1,940,566 + -0.3 1,940,571 + +1.3 1,940,579 - +1.1 1,940,579 - +0.5 1,940,581 + +0.6 1,940,581 - -0.6 1,940,589 - -0.5 1,940,606 - -2.7 1,940,607 - -1.3 1,940,616 - -0.6 1,940,616 - -1.0 1,940,616 - +0.1 1,940,637 - BWI76_RS09190 0.15 -0.6 1,940,638 - BWI76_RS09190 0.15 -0.6 1,940,674 - BWI76_RS09190 0.24 -0.1 1,940,693 - BWI76_RS09190 0.30 -2.3 1,940,693 - BWI76_RS09190 0.30 -0.6 1,940,694 - BWI76_RS09190 0.30 -1.8 1,940,772 - BWI76_RS09190 0.51 -2.7 1,940,772 - BWI76_RS09190 0.51 -1.6 1,940,818 - BWI76_RS09190 0.63 -1.6 1,940,831 - BWI76_RS09190 0.67 -1.0 1,940,863 - BWI76_RS09190 0.75 -0.0 1,941,009 - -2.0 1,941,512 - +2.0 1,941,567 - BWI76_RS09195 0.59 +1.3 1,941,882 - BWI76_RS09195 0.88 +0.7 1,942,098 + BWI76_RS09200 0.14 -0.8 1,942,282 - BWI76_RS09200 0.45 +1.3 1,942,420 + BWI76_RS09200 0.69 +0.3 1,942,420 + BWI76_RS09200 0.69 -1.0 1,942,420 + BWI76_RS09200 0.69 +1.6 1,942,420 + BWI76_RS09200 0.69 -3.3 1,942,428 - BWI76_RS09200 0.70 +0.7 1,942,428 - BWI76_RS09200 0.70 -0.5 1,942,430 + BWI76_RS09200 0.71 -1.1 1,942,430 + BWI76_RS09200 0.71 +1.0 1,942,438 - BWI76_RS09200 0.72 -4.2 1,942,438 - BWI76_RS09200 0.72 +0.8 1,942,438 - BWI76_RS09200 0.72 +0.4 1,942,535 + BWI76_RS09200 0.89 -1.0 1,942,535 + BWI76_RS09200 0.89 +2.5 1,942,543 - -0.2 1,942,543 - -2.0 1,942,543 - +0.9 1,942,543 - +1.2 1,942,545 + -2.3 1,942,545 + -1.0 1,942,545 + +0.2 1,942,545 + +0.6 1,942,545 + -1.2 1,942,545 + -0.6 1,942,545 + +0.6 1,942,545 - +0.2 1,942,553 - +1.8 1,942,553 - +1.0 1,942,553 - +1.1 1,942,553 - -3.8 1,942,553 - -1.3 1,942,553 - +0.2 1,942,553 - +1.4 1,942,553 - +0.6 1,942,553 - -1.5 1,942,553 - -1.2 1,942,553 - +0.9 1,942,553 - -1.9 1,942,553 - -0.1 1,942,553 - -3.6 1,942,589 - +0.2
Or see this region's nucleotide sequence