Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS07865

Experiment: 2-Deoxyadenosine 4 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS07860 and BWI76_RS07865 are separated by 504 nucleotidesBWI76_RS07865 and BWI76_RS07870 are separated by 25 nucleotides BWI76_RS07860: BWI76_RS07860 - cytosine permease, at 1,667,623 to 1,668,885 _RS07860 BWI76_RS07865: BWI76_RS07865 - methylthioribulose 1-phosphate dehydratase, at 1,669,390 to 1,670,001 _RS07865 BWI76_RS07870: BWI76_RS07870 - carbohydrate kinase, at 1,670,027 to 1,671,514 _RS07870 Position (kb) 1669 1670 1671Strain fitness (log2 ratio) -3 -2 -1 0 1at 1668.508 kb on - strandat 1668.903 kb on - strandat 1668.931 kb on + strandat 1668.962 kb on + strandat 1668.984 kb on - strandat 1669.233 kb on - strandat 1669.326 kb on + strandat 1669.484 kb on + strand, within BWI76_RS07865at 1669.568 kb on - strand, within BWI76_RS07865at 1669.731 kb on + strand, within BWI76_RS07865at 1670.161 kb on + strandat 1670.169 kb on - strandat 1670.169 kb on - strandat 1670.422 kb on + strand, within BWI76_RS07870

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C)
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1,668,508 - -0.6
1,668,903 - +0.4
1,668,931 + -3.7
1,668,962 + -1.8
1,668,984 - -0.5
1,669,233 - -0.1
1,669,326 + +0.6
1,669,484 + BWI76_RS07865 0.15 +0.4
1,669,568 - BWI76_RS07865 0.29 -2.7
1,669,731 + BWI76_RS07865 0.56 -1.0
1,670,161 + +0.3
1,670,169 - +0.9
1,670,169 - -0.1
1,670,422 + BWI76_RS07870 0.27 +0.7

Or see this region's nucleotide sequence