Experiment: 2-Deoxyadenosine 4 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05835 and BWI76_RS05840 are separated by 10 nucleotides BWI76_RS05840 and BWI76_RS05845 are separated by 290 nucleotides
BWI76_RS05835: BWI76_RS05835 - gamma-glutamyl-phosphate reductase, at 1,225,173 to 1,226,426
_RS05835
BWI76_RS05840: BWI76_RS05840 - glutamate 5-kinase, at 1,226,437 to 1,227,540
_RS05840
BWI76_RS05845: BWI76_RS05845 - phosphoporin PhoE, at 1,227,831 to 1,228,883
_RS05845
Position (kb)
1226
1227
1228 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1225.528 kb on - strand, within BWI76_RS05835 at 1226.142 kb on + strand, within BWI76_RS05835 at 1226.142 kb on + strand, within BWI76_RS05835 at 1226.310 kb on + strand at 1226.318 kb on + strand at 1226.364 kb on + strand at 1226.501 kb on - strand at 1226.919 kb on - strand, within BWI76_RS05840 at 1226.955 kb on + strand, within BWI76_RS05840 at 1226.980 kb on - strand, within BWI76_RS05840 at 1227.056 kb on - strand, within BWI76_RS05840 at 1227.180 kb on + strand, within BWI76_RS05840 at 1227.356 kb on - strand, within BWI76_RS05840 at 1227.387 kb on + strand, within BWI76_RS05840 at 1227.437 kb on - strand at 1227.469 kb on + strand at 1227.612 kb on + strand at 1227.612 kb on + strand at 1227.612 kb on + strand at 1227.630 kb on + strand at 1227.632 kb on + strand at 1227.638 kb on - strand at 1227.669 kb on + strand at 1227.767 kb on + strand at 1227.775 kb on - strand at 1227.775 kb on - strand at 1227.775 kb on - strand at 1227.787 kb on - strand at 1227.790 kb on + strand at 1227.798 kb on - strand at 1227.798 kb on - strand at 1227.798 kb on - strand at 1227.821 kb on + strand at 1227.821 kb on + strand at 1227.829 kb on - strand at 1227.853 kb on + strand at 1227.865 kb on + strand at 1227.938 kb on - strand, within BWI76_RS05845 at 1227.941 kb on + strand, within BWI76_RS05845 at 1227.949 kb on - strand, within BWI76_RS05845 at 1227.949 kb on - strand, within BWI76_RS05845 at 1227.949 kb on - strand, within BWI76_RS05845 at 1227.954 kb on + strand, within BWI76_RS05845 at 1227.956 kb on + strand, within BWI76_RS05845 at 1227.964 kb on - strand, within BWI76_RS05845 at 1227.964 kb on - strand, within BWI76_RS05845 at 1227.987 kb on + strand, within BWI76_RS05845 at 1227.995 kb on - strand, within BWI76_RS05845 at 1228.000 kb on - strand, within BWI76_RS05845 at 1228.127 kb on - strand, within BWI76_RS05845 at 1228.199 kb on - strand, within BWI76_RS05845 at 1228.253 kb on - strand, within BWI76_RS05845 at 1228.321 kb on + strand, within BWI76_RS05845 at 1228.409 kb on - strand, within BWI76_RS05845 at 1228.446 kb on + strand, within BWI76_RS05845 at 1228.446 kb on + strand, within BWI76_RS05845 at 1228.446 kb on + strand, within BWI76_RS05845 at 1228.446 kb on + strand, within BWI76_RS05845 at 1228.454 kb on - strand, within BWI76_RS05845 at 1228.528 kb on + strand, within BWI76_RS05845
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C) remove 1,225,528 - BWI76_RS05835 0.28 -2.6 1,226,142 + BWI76_RS05835 0.77 -2.8 1,226,142 + BWI76_RS05835 0.77 -1.3 1,226,310 + -2.4 1,226,318 + -4.2 1,226,364 + -1.3 1,226,501 - -1.8 1,226,919 - BWI76_RS05840 0.44 -4.4 1,226,955 + BWI76_RS05840 0.47 -4.6 1,226,980 - BWI76_RS05840 0.49 -4.6 1,227,056 - BWI76_RS05840 0.56 -1.8 1,227,180 + BWI76_RS05840 0.67 -3.4 1,227,356 - BWI76_RS05840 0.83 -1.8 1,227,387 + BWI76_RS05840 0.86 -2.8 1,227,437 - -2.0 1,227,469 + -4.8 1,227,612 + -1.0 1,227,612 + +0.9 1,227,612 + +0.0 1,227,630 + +0.7 1,227,632 + -3.0 1,227,638 - -0.1 1,227,669 + -1.6 1,227,767 + +0.3 1,227,775 - -0.7 1,227,775 - -0.7 1,227,775 - -0.2 1,227,787 - -0.6 1,227,790 + -0.4 1,227,798 - +0.7 1,227,798 - +0.0 1,227,798 - -0.6 1,227,821 + +0.2 1,227,821 + +1.4 1,227,829 - +0.2 1,227,853 + +1.4 1,227,865 + -1.0 1,227,938 - BWI76_RS05845 0.10 +0.7 1,227,941 + BWI76_RS05845 0.10 -0.4 1,227,949 - BWI76_RS05845 0.11 +1.4 1,227,949 - BWI76_RS05845 0.11 +1.0 1,227,949 - BWI76_RS05845 0.11 +0.5 1,227,954 + BWI76_RS05845 0.12 -0.7 1,227,956 + BWI76_RS05845 0.12 -1.3 1,227,964 - BWI76_RS05845 0.13 -0.6 1,227,964 - BWI76_RS05845 0.13 -0.8 1,227,987 + BWI76_RS05845 0.15 -0.0 1,227,995 - BWI76_RS05845 0.16 -1.5 1,228,000 - BWI76_RS05845 0.16 +1.2 1,228,127 - BWI76_RS05845 0.28 -0.7 1,228,199 - BWI76_RS05845 0.35 +0.4 1,228,253 - BWI76_RS05845 0.40 -0.5 1,228,321 + BWI76_RS05845 0.47 +0.9 1,228,409 - BWI76_RS05845 0.55 -0.6 1,228,446 + BWI76_RS05845 0.58 +0.5 1,228,446 + BWI76_RS05845 0.58 -1.3 1,228,446 + BWI76_RS05845 0.58 -2.3 1,228,446 + BWI76_RS05845 0.58 -0.1 1,228,454 - BWI76_RS05845 0.59 +0.3 1,228,528 + BWI76_RS05845 0.66 +0.1
Or see this region's nucleotide sequence