Experiment: 2-Deoxyadenosine 4 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05005 and BWI76_RS05010 are separated by 51 nucleotides BWI76_RS05010 and BWI76_RS05015 are separated by 61 nucleotides BWI76_RS05015 and BWI76_RS05020 are separated by 164 nucleotides BWI76_RS05020 and BWI76_RS05025 overlap by 1 nucleotides
BWI76_RS05005: BWI76_RS05005 - polynucleotide adenylyltransferase, at 1,049,235 to 1,050,632
_RS05005
BWI76_RS05010: BWI76_RS05010 - tRNA glutamyl-Q synthetase, at 1,050,684 to 1,051,565
_RS05010
BWI76_RS05015: BWI76_RS05015 - transcriptional regulator, at 1,051,627 to 1,052,082
_RS05015
BWI76_RS05020: BWI76_RS05020 - sugar fermentation stimulation protein SfsA, at 1,052,247 to 1,052,963
_RS05020
BWI76_RS05025: BWI76_RS05025 - 2'-5' RNA ligase, at 1,052,963 to 1,053,499
_RS05025
Position (kb)
1051
1052
1053 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1050.692 kb on - strand at 1050.692 kb on - strand at 1050.761 kb on + strand at 1050.844 kb on + strand, within BWI76_RS05010 at 1050.844 kb on + strand, within BWI76_RS05010 at 1050.852 kb on - strand, within BWI76_RS05010 at 1051.312 kb on - strand, within BWI76_RS05010 at 1051.541 kb on + strand at 1051.571 kb on + strand at 1051.676 kb on + strand, within BWI76_RS05015 at 1051.867 kb on - strand, within BWI76_RS05015 at 1051.894 kb on - strand, within BWI76_RS05015 at 1051.955 kb on - strand, within BWI76_RS05015 at 1052.065 kb on + strand at 1052.187 kb on - strand at 1052.187 kb on - strand at 1052.187 kb on - strand at 1052.195 kb on - strand at 1052.195 kb on - strand at 1052.197 kb on - strand at 1052.264 kb on + strand at 1052.286 kb on - strand at 1052.291 kb on - strand at 1052.330 kb on + strand, within BWI76_RS05020 at 1052.381 kb on - strand, within BWI76_RS05020 at 1052.382 kb on + strand, within BWI76_RS05020 at 1052.390 kb on - strand, within BWI76_RS05020 at 1052.395 kb on + strand, within BWI76_RS05020 at 1052.395 kb on + strand, within BWI76_RS05020 at 1052.426 kb on - strand, within BWI76_RS05020 at 1052.524 kb on + strand, within BWI76_RS05020 at 1052.531 kb on - strand, within BWI76_RS05020 at 1052.543 kb on + strand, within BWI76_RS05020 at 1052.656 kb on + strand, within BWI76_RS05020 at 1052.779 kb on - strand, within BWI76_RS05020 at 1052.809 kb on - strand, within BWI76_RS05020 at 1052.823 kb on - strand, within BWI76_RS05020 at 1052.851 kb on + strand, within BWI76_RS05020 at 1052.860 kb on - strand, within BWI76_RS05020 at 1052.862 kb on + strand, within BWI76_RS05020 at 1052.871 kb on - strand, within BWI76_RS05020 at 1052.881 kb on - strand, within BWI76_RS05020
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C) remove 1,050,692 - -2.2 1,050,692 - -0.5 1,050,761 + -0.9 1,050,844 + BWI76_RS05010 0.18 -0.8 1,050,844 + BWI76_RS05010 0.18 +0.2 1,050,852 - BWI76_RS05010 0.19 +0.3 1,051,312 - BWI76_RS05010 0.71 -2.4 1,051,541 + +0.2 1,051,571 + -0.1 1,051,676 + BWI76_RS05015 0.11 +1.6 1,051,867 - BWI76_RS05015 0.53 +0.9 1,051,894 - BWI76_RS05015 0.59 -3.0 1,051,955 - BWI76_RS05015 0.72 +1.5 1,052,065 + -1.4 1,052,187 - +0.3 1,052,187 - -1.4 1,052,187 - +0.2 1,052,195 - -0.3 1,052,195 - -0.3 1,052,197 - -2.4 1,052,264 + +1.1 1,052,286 - +0.1 1,052,291 - -0.5 1,052,330 + BWI76_RS05020 0.12 +0.1 1,052,381 - BWI76_RS05020 0.19 +0.1 1,052,382 + BWI76_RS05020 0.19 -1.5 1,052,390 - BWI76_RS05020 0.20 -0.1 1,052,395 + BWI76_RS05020 0.21 +0.4 1,052,395 + BWI76_RS05020 0.21 +0.7 1,052,426 - BWI76_RS05020 0.25 -1.0 1,052,524 + BWI76_RS05020 0.39 -0.9 1,052,531 - BWI76_RS05020 0.40 +1.2 1,052,543 + BWI76_RS05020 0.41 -0.6 1,052,656 + BWI76_RS05020 0.57 +0.4 1,052,779 - BWI76_RS05020 0.74 +0.7 1,052,809 - BWI76_RS05020 0.78 -0.5 1,052,823 - BWI76_RS05020 0.80 +0.0 1,052,851 + BWI76_RS05020 0.84 +0.7 1,052,860 - BWI76_RS05020 0.85 +0.5 1,052,862 + BWI76_RS05020 0.86 -0.1 1,052,871 - BWI76_RS05020 0.87 -0.9 1,052,881 - BWI76_RS05020 0.88 +0.2
Or see this region's nucleotide sequence